cDNA Clone Sic1

• Plasmid: pRS413-Gal1 Pr-HA-3Gly-Sic1
• Parent Plasmid: pRS413
• Expressed: HA-Sic1
• Genbank: 850768
• Tag: HA
• Species: Saccharomyces cerevisiae

Sequence of Insert

GGATCCATGACTCCTTCCACCCCACCAAGGTCCAGAGGGACTAGGTACCT
TGCGCAGCCTAGTGGCAATACTAGTTCTAGTGCCCTAATGCAAGGTCAAA
AGACCCCCCAAAAGCCTTCACAGAACCTAGTCCCTGTCACTCCCTCAACA
ACTAAGTCTTTTAAAAATGCGCCATTATTAGCACCTCCCAATTCGAACAT
GGGTATGACCTCTCCATTTAATGGGCTTACGTCTCCTCAACGCTCGCCGT
TTCCAAAATCTTCAGTGAAGAGGACACTATTCCAATTTGAAAGTCATGAT
AATGGAACAGTAAGGGAAGAGCAGGAACCATTGGGTCGTGTAAATAGGAT
ATTGTTTCCCACGCAGCAAAATGTGGATATAGATGCAGCAGAAGAAGAAG
AAGAAGGAGAAGTTCTTCTTCCCCCCAGCAGACCTACATCTGCCAGGCAG
TTACATTTATCACTTGAAAGAGATGAGTTTGATCAGACACATAGAAAGAA
GATTATTAAAGATGTACCTGGTACGCCCAGCGACAAGGTGATAACATTTG
AATTGGCAAAAAATTGGAACAACAACTCTCCGAAAAATGACGCCAGGAGT
CAAGAAAGTGAAGACGAGGAAGACATCATCATCAATCCAGTGCGGGTGGG
TAAAAATCCCTTTGCATCAGATGAACTGGTCACTCAGGAAATTAGAAATG
AACGTAAAAGGGCAATGTTGAGAGAAAACCCAGATATAGAAGACGTAATA
ACATATGTCAATAAGAAGGGAGAGGTGGTAGAGAAACGAAGGTTAACGGA
TGAAGAAAAGAGAAGATTCAAGCCAAAGGCATTGTTTCAATCTAGGGATC
AAGAGCATTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGGGGSMTPSTPPRSRGTRYLAQPSGNTSSSALMQGQKTPQ
KPSQNLVPVTPSTTKSFKNAPLLAPPNSNMGMTSPFNGLTSPQRSPFPKS
SVKRTLFQFESHDNGTVREEQEPLGRVNRILFPTQQNVDIDAAEEEEEGE
VLLPPSRPTSARQLHLSLERDEFDQTHRKKIIKDVPGTPSDKVITFELAK
NWNNNSPKNDARSQESEDEEDIIINPVRVGKNPFASDELVTQEIRNERKR
AMLRENPDIEDVITYVNKKGEVVEKRRLTDEEKRRFKPKALFQSRDQEH*
AA

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU20822
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: yeast expression vector for N-terminally HA tagged Sic1 (with glycine linker) driven by Gal1,10 Promoter