cDNA Clone Sic1

• Plasmid: pYES2-3HA-Sic1
• Parent Plasmid: pYES2
• Expressed: 3HA-Sic1
• Tag: HA
• Species: Saccharomyces cerevisiae

Sequence of Insert

GCGGCCGCgATGACTCCTTCCACCCCACCAAGGTCCAGAGGGACTAGGTA
CCTTGCGCAGCCTAGTGGCAATACTAGTTCTAGTGCCCTAATGCAAGGTC
AAAAGACCCCCCAAAAGCCTTCACAGAACCTAGTCCCTGTCACTCCCTCA
ACAACTAAGTCTTTTAAAAATGCGCCATTATTAGCACCTCCCAATTCGAA
CATGGGTATGACCTCTCCATTTAATGGGCTTACGTCTCCTCAACGCTCGC
CGTTTCCAAAATCTTCAGTGAAGAGGACACTATTCCAATTTGAAAGTCAT
GATAATGGAACAGTAAGGGAAGAGCAGGAACCATTGGGTCGTGTAAATAG
GATATTGTTTCCCACGCAGCAAAATGTGGATATAGATGCAGCAGAAGAAG
AAGAAGAAGGAGAAGTTCTTCTTCCCCCCAGCAGACCTACATCTGCCAGG
CAGTTACATTTATCACTTGAAAGAGATGAGTTTGATCAGACACATAGAAA
GAAGATTATTAAAGATGTACCTGGTACGCCCAGCGACAAGGTGATAACAT
TTGAATTGGCAAAAAATTGGAACAACAACTCTCCGAAAAATGACGCCAGG
AGTCAAGAAAGTGAAGACGAGGAAGACATCATCATCAATCCAGTGCGGGT
GGGTAAAAATCCCTTTGCATCAGATGAACTGGTCACTCAGGAAATTAGAA
ATGAACGTAAAAGGGCAATGTTGAGAGAAAACCCAGATATAGAAGACGTA
ATAACATATGTCAATAAGAAGGGAGAGGTGGTAGAGAAACGAAGGTTAAC
GGATGAAGAAAAGAGAAGATTCAAGCCAAAGGCATTGTTTCAATCTAGGG
ATCAAGAGCATTGAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAMYPYDVPDYAMYPYDVPDYAAAAMTPSTPPRSRGTRYLAQ
PSGNTSSSALMQGQKTPQKPSQNLVPVTPSTTKSFKNAPLLAPPNSNMGM
TSPFNGLTSPQRSPFPKSSVKRTLFQFESHDNGTVREEQEPLGRVNRILF
PTQQNVDIDAAEEEEEGEVLLPPSRPTSARQLHLSLERDEFDQTHRKKII
KDVPGTPSDKVITFELAKNWNNNSPKNDARSQESEDEEDIIINPVRVGKN
PFASDELVTQEIRNERKRAMLRENPDIEDVITYVNKKGEVVEKRRLTDEE
KRRFKPKALFQSRDQEH*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU20694
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Yeast vector for Gal1-driven expression of N-terminally tagged 3HA-Sic1; Parental PLasmid SC20671 (pYES2 derived); Estimated MW of expressed protein : 36083 Daltons