cDNA Clone Cullin2

• Plasmid: pEFIRES-P-eYFP C1-Cullin2
• Parent Plasmid: pEFIRES puro eYFPC1
• Expressed: eYFP-Cullin2
• Genbank: NM_003591
• Tag: YFP
• Species: Human

Sequence of Insert

GGATCCATGTCTTTGAAACCAAGAGTAGTAGATTTTGATGAAACATGGAACAAACTTTTGACGACAATAAAAGCCGTGGTCATGTTGGAATACGTCGAAAGAGCAACATGGAATGACCGTTTCTCAGATATCTATGCTTTATGTGTGGCCTATCCTGAACCCCTTGGAGAAAGACTTTATACAGAAACTAAGATTTTTTTGGAAAATCATGTTCGGCATTTGCATAAGAGAGTTTTGGAGTCAGAAGAACAAGTACTTGTTATGTATCATAGGTACTGGGAAGAATACAGCAAGGGTGCAGACTAT
ATGGACTGCTTATATAGGTATCTCAACACCCAGTTTATTAAAAAGAATAA
ATTAACAGAAGCGGACCTTCAGTATGGCTATGGTGGTGTAGATATGAATG
AACCACTTATGGAAATAGGAGAGCTAGCATTGGATATGTGGAGGAAATTG
ATGGTTGAACCACTTCAGGCCATCCTTATCCGAATGCTGCTCCGAGAAAT
CAAAAATGATCGTGGTGGAGAAGACCCAAACCAGAAAGTAATCCATGGGG
TTATTAACTCCTTTGTTCATGTTGAACAGTATAAGAAAAAATTCCCCTTA
AAGTTTTATCAGGAAATTTTTGAGTCTCCCTTTCTGACTGAAACAGGAGA
GTATTACAAACAAGAAGCTTCAAATTTATTACAAGAATCAAACTGCTCAC
AGTATATGGAAAAGGTTCTAGGTAGATTAAAAGATGAAGAAATTCGATGT
CGAAAATACCTACATCCAAGTTCATATACTAAGGTGATTCATGAATGTCA
ACAACGAATGGTAGCAGACCACTTACAGTTTTTACATGCAGAATGTCATA
ATATAATTCGACAAGAGAAAAAAAATGACATGGCAAATATGTACGTCTTA
CTCCGTGCTGTGTCCACTGGTTTACCTCATATGATTCAGGAGCTGCAAAA
CCACATCCATGATGAGGGCCTTCGAGCAACCAGCAACCTTACTCAGGAAA
ACATGCCAACACTATTTGTGGAGTCAGTTTTGGAAGTGCATGGTAAATTT
GTTCAGCTTATCAACACTGTTTTGAATGGTGATCAGCATTTTATGAGTGC
GTTGGATAAGGCCCTTACGTCAGTTGTAAATTACAGAGAACCTAAGTCTG
TTTGCAAAGCACCTGAACTGCTTGCTAAGTACTGTGACAACTTACTGAAG
AAGTCAGCGAAAGGGATGACAGAGAATGAAGTGGAAGACAGGCTCACGAG
CTTCATCACAGTGTTCAAATACATTGATGACAAGGACGTCTTTCAAAAGT
TCTACGCAAGAATGCTGGCAAAACGTTTAATTCATGGGTTATCCATGTCT
ATGGACTCTGAAGAAGCCATGATCAACAAATTAAAGCAAGCCTGTGGTTA
TGAGTTTACCAGCAAGCTACATCGGATGTATACAGATATGAGTGTCAGCG
CTGATCTCAACAATAAGTTCAACAATTTTATCAAAAACCAAGACACAGTA
ATAGATTTGGGAATTAGTTTTCAAATATATGTTCTACAGGCTGGTGCGTG
GCCTCTTACTCAGGCTCCTTCATCTACGTTTGCAATTCCCCAGGAATTAG
AAAAAAGTGTACAGATGTTTGAATTATTTTATAGCCAACATTTCAGTGGA
AGGAAACTTACATGGTTACATTATCTGTGTACAGGTGAAGTTAAAATGAA
CTATTTGGGCAAACCATATGTAGCCATGGTTACAACATACCAAATGGCAG
TTCTTCTTGCCTTTAACAACAGTGAAACTGTCAGTTATAAAGAGCTTCAG
GACAGCACTCAGATGAATGAAAAGGAACTGACAAAAACAATCAAATCATT
ACTTGATGTGAAAATGATTAACCATGATTCAGAAAAGGAAGATATTGATG
CAGAATCTTCGTTTTCATTAAATATGAACTTTAGCAGTAAAAGAACAAAA
TTTAAAATTACTACATCAATGCAGAAAGACACACCACAAGAAATGGAGCA
GACTAGAAGTGCAGTTGATGAGGACCGGAAAATGTATCTCCAAGCTGCTA
TAGTTCGTATCATGAAAGCACGAAAAGTGCTTCGGCACAATGCCCTTATT
CAAGAGGTGATTAGCCAGTCAAGAGCTAGGTTTAATCCCAGTATCAGCAT
GATTAAGAAGTGTATTGAAGTTCTGATAGACAAACAATACATAGAACGCA
GCCAGGCGTCGGCAGATGAATACAGCTACGTCGCGTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSMSLKPR
VVDFDETWNKLLTTIKAVVMLEYVERATWNDRFSDIYALCVAYPEPLGER
LYTETKIFLENHVRHLHKRVLESEEQVLVMYHRYWEEYSKGADYMDCLYR
YLNTQFIKKNKLTEADLQYGYGGVDMNEPLMEIGELALDMWRKLMVEPLQ
AILIRMLLREIKNDRGGEDPNQKVIHGVINSFVHVEQYKKKFPLKFYQEI
FESPFLTETGEYYKQEASNLLQESNCSQYMEKVLGRLKDEEIRCRKYLHP
SSYTKVIHECQQRMVADHLQFLHAECHNIIRQEKKNDMANMYVLLRAVST
GLPHMIQELQNHIHDEGLRATSNLTQENMPTLFVESVLEVHGKFVQLINT
VLNGDQHFMSALDKALTSVVNYREPKSVCKAPELLAKYCDNLLKKSAKGM
TENEVEDRLTSFITVFKYIDDKDVFQKFYARMLAKRLIHGLSMSMDSEEA
MINKLKQACGYEFTSKLHRMYTDMSVSADLNNKFNNFIKNQDTVIDLGIS
FQIYVLQAGAWPLTQAPSSTFAIPQELEKSVQMFELFYSQHFSGRKLTWL
HYLCTGEVKMNYLGKPYVAMVTTYQMAVLLAFNNSETVSYKELQDSTQMN
EKELTKTIKSLLDVKMINHDSEKEDIDAESSFSLNMNFSSKRTKFKITTS
MQKDTPQEMEQTRSAVDEDRKMYLQAAIVRIMKARKVLRHNALIQEVISQ
SRARFNPSISMIKKCIEVLIDKQYIERSQASADEYSYVA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20543
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression vector for eYFP-Cullin2. EF1a promoter, IRES and puromycin selection cassette; ; Estimated MW of expressed protein : 114458 Daltons