cDNA Clone NPL4

• Plasmid: pCMV5-FLAG NPL4 K350R
• Parent Plasmid: pCMV5 FLAG3
• Expressed: FLAG-NPL4 K350R
• Genbank: NM_017921*
• Tag: FLAG
• Species: Human

Sequence of Insert

GTCGACATGGCCGAGAGCATCATAATTCGTGTCCAGTCCCCGGATGGAGT
GAAGCGGATCACAGCAACAAAGAGAGAAACAGCAGCAACATTTTTGAAAA
AGGTTGCAAAGGAGTTTGGCTTCCAAAATAATGGCTTCTCGGTTTACATC
AATAGAAACAAGACCGGAGAGATAACAGCCTCCTCCAACAAATCCCTCAA
CTTGCTAAAAATCAAGCATGGCGATTTGTTGTTCCTGTTTCCCTCGAGCC
TTGCTGGGCCCTCATCTGAAATGGAGACTTCAGTTCCACCGGGCTTCAAA
GTCTTTGGCGCTCCCAACGTGGTGGAGGATGAGATTGATCAGTACCTCAG
CAAACAGGACGGGAAGATTTACAGAAGCCGAGACCCACAGCTATGCCGCC
ACGGCCCTTTGGGGAAATGCGTGCACTGCGTCCCTCTAGAGCCATTCGAT
GAGGACTATCTAAACCATCTCGAGCCTCCCGTGAAGCACATGTCCTTCCA
CGCCTACATCCGGAAGCTGACTGGAGGGGCTGACAAGGGGAAGTTTGTTG
CCCTGGAGAACATCAGCTGCAAGATTAAGTCAGGGTGCGAGGGGCACCTC
CCGTGGCCGAATGGCATCTGTACTAAGTGCCAGCCGAGCGCCATCACGCT
GAACAGACAGAAGTACAGGCATGTGGACAATATCATGTTTGAGAATCACA
CCGTCGCTGACCGCTTTCTTGACTTCTGGAGAAAGACAGGGAACCAGCAT
TTTGGGTACTTATACGGACGGTACACGGAGCACAAAGACATTCCCCTTGG
CATCAGGGCTGAAGTGGCTGCGATTTATGAGCCACCTCAGATTGGTACAC
AGAACAGCTTGGAGCTTCTTGAGGATCCAAAAGCTGAAGTGGTCGATGAA
ATTGCTGCCAAACTTGGCCTGCGGAAGGTTGGCTGGATATTTACAGACCT
CGTCTCAGAAGATACCCGAAAGGGTACCGTCCGCTACAGTCGAAATAAGG
ACACCTATTTCCTAAGTTCAGAAGAGTGCATCACTGCAGGAGACTTCCAG
AACAGGCATCCCAACATGTGCCGGCTCTCTCCAGACGGACATTTTGGATC
CAAGTTTGTTACTGCAGTGGCTACAGGTGGTCCTGACAACCAAGTCCACT
TTGAAGGGTACCAGGTGTCCAATCAGTGTATGGCACTGGTCCGTGATGAG
TGTTTGCTGCCATGCAAGGACGCCCCGGAGCTTGGCTACGCCAAGGAGTC
TAGCAGTGAGCAGTACGTGCCTGATGTGTTTTATAAGGACGTAGACAAGT
TTGGCAACGAGATCACCCAGCTGGCCCGTCCCCTGCCTGTGGAGTATCTC
ATCATAGACATCACAACAACTTTCCCCAAGGATCCAGTTTACACTTTTTC
TATTTCGCAAAATCCATTTCCTATTGAAAACCGGGATGTATTGGGTGAGA
CACAGGACTTCCATAGCTTGGCCACCTATTTGTCTCAGAATACCTCATCT
GTGTTCTTGGATACCATCTCAGATTTCCACCTCTTGCTGTTCCTGGTCAC
CAATGAAGTTATGCCTCTGCAGGACAGCATCAGCTTGCTGCTGGAGGCCG
TGCGGACCAGAAATGAGGAGCTCGCCCAGACATGGAAGAGGTCTGAGCAG
TGGGCCACCATCGAGCAGCTGTGCAGCACAGTTGGCGGGCAGCTCCCAGG
TCTCCATGAGTACGGGGCCGTCGGGGGCTCCACACACACGGCCACTGCAG
CCATGTGGGCCTGTCAGCACTGCACGTTCATGAACCAGCCAGGCACAGGC
CACTGCGAGATGTGCAGCCTCCCCAGGACCTAGGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGDPRNSRVDMAESIIIRVQSPDGVKRITATKRETAATFLKK
VAKEFGFQNNGFSVYINRNKTGEITASSNKSLNLLKIKHGDLLFLFPSSL
AGPSSEMETSVPPGFKVFGAPNVVEDEIDQYLSKQDGKIYRSRDPQLCRH
GPLGKCVHCVPLEPFDEDYLNHLEPPVKHMSFHAYIRKLTGGADKGKFVA
LENISCKIKSGCEGHLPWPNGICTKCQPSAITLNRQKYRHVDNIMFENHT
VADRFLDFWRKTGNQHFGYLYGRYTEHKDIPLGIRAEVAAIYEPPQIGTQ
NSLELLEDPKAEVVDEIAAKLGLRKVGWIFTDLVSEDTRKGTVRYSRNKD
TYFLSSEECITAGDFQNRHPNMCRLSPDGHFGSKFVTAVATGGPDNQVHF
EGYQVSNQCMALVRDECLLPCKDAPELGYAKESSSEQYVPDVFYKDVDKF
GNEITQLARPLPVEYLIIDITTTFPKDPVYTFSISQNPFPIENRDVLGET
QDFHSLATYLSQNTSSVFLDTISDFHLLLFLVTNEVMPLQDSISLLLEAV
RTRNEELAQTWKRSEQWATIEQLCSTVGGQLPGLHEYGAVGGSTHTATAA
MWACQHCTFMNQPGTGHCEMCSLPRT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20132
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N-terminally FLAG tagged NPL4 K350R for mammalian expression.; In addition to K350R substitution this clone harbours four point mutations with respect to ref sequence (all silent). t159c (Thr53), g273t (Thr91), g1323t (Arg441), c1710g (Gly570).; ; Estimated MW of expressed protein : 70244 Daltons