cDNA Clone NPL4

• Plasmid: pCMV5-FLAG NPL4 K331R
• Parent Plasmid: pCMV5 FLAG3
• Expressed: FLAG-NPL4 K331R
• Genbank: NM_017921*
• Tag: FLAG
• Species: Human

Sequence of Insert

GTCGACATGGCCGAGAGCATCATAATTCGTGTCCAGTCCCCGGATGGAGT
GAAGCGGATCACAGCAACAAAGAGAGAAACAGCAGCAACATTTTTGAAAA
AGGTTGCAAAGGAGTTTGGCTTCCAAAATAATGGCTTCTCGGTTTACATC
AATAGAAACAAGACCGGAGAGATAACAGCCTCCTCCAACAAATCCCTCAA
CTTGCTAAAAATCAAGCATGGCGATTTGTTGTTCCTGTTTCCCTCGAGCC
TTGCTGGGCCCTCATCTGAAATGGAGACTTCAGTTCCACCGGGCTTCAAA
GTCTTTGGCGCTCCCAACGTGGTGGAGGATGAGATTGATCAGTACCTCAG
CAAACAGGACGGGAAGATTTACAGAAGCCGAGACCCACAGCTATGCCGCC
ACGGCCCTTTGGGGAAATGCGTGCACTGCGTCCCTCTAGAGCCATTCGAT
GAGGACTATCTAAACCATCTCGAGCCTCCCGTGAAGCACATGTCCTTCCA
CGCCTACATCCGGAAGCTGACTGGAGGGGCTGACAAGGGGAAGTTTGTTG
CCCTGGAGAACATCAGCTGCAAGATTAAGTCAGGGTGCGAGGGGCACCTC
CCGTGGCCGAATGGCATCTGTACTAAGTGCCAGCCGAGCGCCATCACGCT
GAACAGACAGAAGTACAGGCATGTGGACAATATCATGTTTGAGAATCACA
CCGTCGCTGACCGCTTTCTTGACTTCTGGAGAAAGACAGGGAACCAGCAT
TTTGGGTACTTATACGGACGGTACACGGAGCACAAAGACATTCCCCTTGG
CATCAGGGCTGAAGTGGCTGCGATTTATGAGCCACCTCAGATTGGTACAC
AGAACAGCTTGGAGCTTCTTGAGGATCCAAAAGCTGAAGTGGTCGATGAA
ATTGCTGCCAAACTTGGCCTGCGGAAGGTTGGCTGGATATTTACAGACCT
CGTCTCAGAAGATACCCGAAAGGGTACCGTCCGCTACAGTCGAAATAGGG
ACACCTATTTCCTAAGTTCAGAAGAGTGCATCACTGCAGGAGACTTCCAG
AACAAGCATCCCAACATGTGCCGGCTCTCTCCAGACGGACATTTTGGATC
CAAGTTTGTTACTGCAGTGGCTACAGGTGGTCCTGACAACCAAGTCCACT
TTGAAGGGTACCAGGTGTCCAATCAGTGTATGGCACTGGTCCGTGATGAG
TGTTTGCTGCCATGCAAGGACGCCCCGGAGCTTGGCTACGCCAAGGAGTC
TAGCAGTGAGCAGTACGTGCCTGATGTGTTTTATAAGGACGTAGACAAGT
TTGGCAACGAGATCACCCAGCTGGCCCGTCCCCTGCCTGTGGAGTATCTC
ATCATAGACATCACAACAACTTTCCCCAAGGATCCAGTTTACACTTTTTC
TATTTCGCAAAATCCATTTCCTATTGAAAACCGGGATGTATTGGGTGAGA
CACAGGACTTCCATAGCTTGGCCACCTATTTGTCTCAGAATACCTCATCT
GTGTTCTTGGATACCATCTCAGATTTCCACCTCTTGCTGTTCCTGGTCAC
CAATGAAGTTATGCCTCTGCAGGACAGCATCAGCTTGCTGCTGGAGGCCG
TGCGGACCAGAAATGAGGAGCTCGCCCAGACATGGAAGAGGTCTGAGCAG
TGGGCCACCATCGAGCAGCTGTGCAGCACAGTTGGCGGGCAGCTCCCAGG
TCTCCATGAGTACGGGGCCGTCGGGGGCTCCACACACACGGCCACTGCAG
CCATGTGGGCCTGTCAGCACTGCACGTTCATGAACCAGCCAGGCACAGGC
CACTGCGAGATGTGCAGCCTCCCCAGGACCTAGGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGDPRNSRVDMAESIIIRVQSPDGVKRITATKRETAATFLKK
VAKEFGFQNNGFSVYINRNKTGEITASSNKSLNLLKIKHGDLLFLFPSSL
AGPSSEMETSVPPGFKVFGAPNVVEDEIDQYLSKQDGKIYRSRDPQLCRH
GPLGKCVHCVPLEPFDEDYLNHLEPPVKHMSFHAYIRKLTGGADKGKFVA
LENISCKIKSGCEGHLPWPNGICTKCQPSAITLNRQKYRHVDNIMFENHT
VADRFLDFWRKTGNQHFGYLYGRYTEHKDIPLGIRAEVAAIYEPPQIGTQ
NSLELLEDPKAEVVDEIAAKLGLRKVGWIFTDLVSEDTRKGTVRYSRNRD
TYFLSSEECITAGDFQNKHPNMCRLSPDGHFGSKFVTAVATGGPDNQVHF
EGYQVSNQCMALVRDECLLPCKDAPELGYAKESSSEQYVPDVFYKDVDKF
GNEITQLARPLPVEYLIIDITTTFPKDPVYTFSISQNPFPIENRDVLGET
QDFHSLATYLSQNTSSVFLDTISDFHLLLFLVTNEVMPLQDSISLLLEAV
RTRNEELAQTWKRSEQWATIEQLCSTVGGQLPGLHEYGAVGGSTHTATAA
MWACQHCTFMNQPGTGHCEMCSLPRT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20131
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: N-terminally FLAG tagged NPL4 K331R mutant for mammalian expression.; In addition to K331R substitution this clone harbours four point mutations with respect to ref sequence (all silent). t159c (Thr53), g273t (Thr91), g1323t (Arg441), c1710g (Gly570).; ; Estimated MW of expressed protein : 70244 Daltons