cDNA Clone DUBA-1

• Plasmid: pFBHT-DUBA-1
• Parent Plasmid: pFastBac HTb
• Expressed: 6His-DUBA-1
• Genbank: NM_017602*
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGACTATACTCCCCAAAAAGAAGCCGCCGCCTCCCGACGCCGACCCCGGCGGCACGGGCGTGGGCGGCGGCGATCGCGACCGTGACTCCGGCGTCGTGGGGGCCCGTCCGCGAGCTTCGCCACCGCCTCAAGGCCCGCTACCAGGACCGCCGGGCGCTCTTCATCGCTGGGCGCTGGCCGTGCCGCCTGGTGCAGTGGCGGGTCCCCGGCCACAACAGGCTTCTCCACCTCCTTGCGGGGGCCCAGGTGGTCCCGGCGGCGGTCCCGGCGACGCGCTGGGCGCAGCGGCGGCGGGTGTG
GGTGCCGCGGGCGTGGTGGTGGGTGTGGGTGGTGCCGTAGGCGTGGGCGG
CTGCTGCTCCGGGCCTGGGCACAGCAAGCGGCGACGTCAAGCTCCCGGGG
TTGGCGCGGTTGGCGGGGGCAGTCCCGAGCGTGAGGAGGTCGGCGCAGGC
TACAACAGTGAGGACGAGTATGAGGCGGCTGCAGCACGCATCGAGGCTAT
GGACCCTGCCACTGTCGAGCAGCAGGAGCATTGGTTTGAAAAGGCCCTAC
GAGACAAGAAGGGCTTCATCATCAAGCAGATGAAGGAGGATGGCGCCTGT
CTCTTCCGGGCTGTAGCTGACCAGGTGTATGGAGACCAGGACATGCATGA
GGTTGTGCGAAAGCATTGCATGGACTATCTGATGAAGAATGCCGACTACT
TCTCCAACTATGTCACAGAGGACTTTACCACCTACATTAACAGGAAGCGG
AAAAACAATTGCCATGGCAACCACATTGAGATGCAGGCCATGGCAGAGAT
GTACAACCGTCCTGTGGAGGTGTACCAGTACAGCACAGAACCCATCAACA
CATTCCATGGGATACATCAAAACGAGGACGAACCCATTCGTGTTAGCTAC
CATCGGAATATCCACTATAATTCAGTGGTGAATCCTAACAAGGCCACCAT
TGGTGTGGGGCTGGGCCTGCCATCATTCAAACCAGGGTTTGCAGAGCAGT
CTCTGATGAAGAATGCCATAAAAACATCGGAGGAGTCATGGATTGAACAG
CAGATGCTAGAAGACAAGAAACGGGCCACAGACTGGGAGGCCACAAATGA
AGCCATCGAGGAGCAGGTGGCTCGGGAATCCTACCTGCAGTGGTTGCGGG
ATCAGGAGAAACAGGCTCGCCAGGTCCGAGGCCCCAGCCAGCCCCGGAAA
GCCAGCGCCACATGCAGTTCGGCCACAGCAGCAGCCTCCAGTGGCCTGGA
GGAGTGGACTAGCCGGTCCCCGCGGCAGCGGAGTTCAGCCTCGTCACCTG
AGCACCCTGAGCTGCATGCTGAATTGGGCATGAAGCCCCCTTCCCCAGGC
ACTGTTTTAGCTCTTGCCAAACCTCCTTCGCCCTGTGCGCCAGGTACAAG
CAGTCAGTTCTCGGCAGGGGCCGACCGGGCAACTTCCCCCCTTGTGTCCC
TCTACCCTGCTTTGGAGTGCCGGGCCCTCATTCAGCAGATGTCCCCCTCT
GCCTTTGGTCTGAATGACTGGGATGATGATGAGATCCTAGCTTCGGTGCT
GGCAGTGTCCCAACAGGAATACCTAGACAGTATGAAGAAAAACAAAGTGC
ACAGAGACCCGCCCCCAGACAAGAGTTGAGCGGCCGC

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMGSMTILPKKKPPPPDADPGGTGVG
GGDRDRDSGVVGARPRASPPPQGPLPGPPGALHRWALAVPPGAVAGPRPQ
QASPPPCGGPGGPGGGPGDALGAAAAGVGAAGVVVGVGGAVGVGGCCSGP
GHSKRRRQAPGVGAVGGGSPEREEVGAGYNSEDEYEAAAARIEAMDPATV
EQQEHWFEKALRDKKGFIIKQMKEDGACLFRAVADQVYGDQDMHEVVRKH
CMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHGNHIEMQAMAEMYNRPV
EVYQYSTEPINTFHGIHQNEDEPIRVSYHRNIHYNSVVNPNKATIGVGLG
LPSFKPGFAEQSLMKNAIKTSEESWIEQQMLEDKKRATDWEATNEAIEEQ
VARESYLQWLRDQEKQARQVRGPSQPRKASATCSSATAAASSGLEEWTSR
SPRQRSSASSPEHPELHAELGMKPPSPGTVLALAKPPSPCAPGTSSQFSA
GADRATSPLVSLYPALECRALIQQMSPSAFGLNDWDDDEILASVLAVSQQ
EYLDSMKKNKVHRDPPPDKS*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU20022
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus construct to express DUBA-1 with N-terminal 6HIS tag (contains TEV protease site); ; Estimated MW of expressed protein : 61379 Daltons