cDNA Clone UBC1

• Plasmid: pET3D-UBC1
• Parent Plasmid: pET3d
• Expressed: untagged UBC1
• Genbank: NM_005339
• Tag: untagged
• Species: Human

Sequence of Insert

CCATGGCCAACATCGCGGTGCAGCGAATCAAGCGGGAGTTCAAGGAGGTGCTGAAGAGCGAGGAGACGAGCAAAAATCAAATTAAAGTAGATCTTGTAGATG
AGAATTTTACAGAATTAAGAGGAGAAATAGCAGGACCTCCAGACACACCA
TATGAAGGAGGAAGATACCAACTAGAGATAAAAATACCAGAAACATACCC
ATTTAATCCCCCTAAGGTCCGGTTTATCACTAAAATATGGCATCCTAATA
TTAGTTCCGTCACAGGGGCTATTTGTTTGGATATCCTGAAAGATCAATGG
GCAGCTGCAATGACTCTCCGCACGGTATTATTGTCATTGCAAGCACTATT
GGCAGCTGCAGAGCCAGATGATCCACAGGATGCTGTAGTAGCAAATCAGT
ACAAACAAAATCCCGAAATGTTCAAACAGACAGCTCGACTTTGGGCACAT
GTGTATGCTGGAGCACCAGTTTCTAGTCCAGAATACACCAAAAAAATAGA
AAACCTATGTGCTATGGGCTTTGATAGGAATGCAGTAATAGTGGCCTTGT
CTTCAAAATCATGGGATGTAGAGACTGCAACAGAATTGCTTCTGAGTAAC
TGAGGATCC

Amino Acid Sequence

MANIAVQRIKREFKEVLKSEETSKNQIKVDLVDENFTELRGEIAGPPDTP
YEGGRYQLEIKIPETYPFNPPKVRFITKIWHPNISSVTGAICLDILKDQW
AAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAH
VYAGAPVSSPEYTKKIENLCAMGFDRNAVIVALSSKSWDVETATELLLSN

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20019
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for untagged Ubc1 (E2-25K) ; ; Estimated MW of expressed protein : 22407 Daltons