cDNA Clone UBC1

• Plasmid: pET156P-UBC1
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-UBC1
• Genbank: NM_005339
• Tag: 6His
• Species: Human

Sequence of Insert

GGATCCATGGCCAACATCGCGGTGCAGCGAATCAAGCGGGAGTTCAAGGA
GGTGCTGAAGAGCGAGGAGACGAGCAAAAATCAAATTAAAGTAGATCTTG
TAGATGAGAATTTTACAGAATTAAGAGGAGAAATAGCAGGACCTCCAGAC
ACACCATATGAAGGAGGAAGATACCAACTAGAGATAAAAATACCAGAAAC
ATACCCATTTAATCCCCCTAAGGTCCGGTTTATCACTAAAATATGGCATC
CTAATATTAGTTCCGTCACAGGGGCTATTTGTTTGGATATCCTGAAAGAT
CAATGGGCAGCTGCAATGACTCTCCGCACGGTATTATTGTCATTGCAAGC
ACTATTGGCAGCTGCAGAGCCAGATGATCCACAGGATGCTGTAGTAGCAA
ATCAGTACAAACAAAATCCCGAAATGTTCAAACAGACAGCTCGACTTTGG
GCACATGTGTATGCTGGAGCACCAGTTTCTAGTCCAGAATACACCAAAAA
AATAGAAAACCTATGTGCTATGGGCTTTGATAGGAATGCAGTAATAGTGG
CCTTGTCTTCAAAATCATGGGATGTAGAGACTGCAACAGAATTGCTTCTG
AGTAACTGAGCGGCCGC

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMANIAVQRIKREFKEVLKSEETSKNQIKVDLVDENFTELRGEIAGPPDTPYEGGRYQLEIKIPETYPFNPPKVRFITKIWHPNISSVTGAICLDILKDQWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYAGAPVSSPEYTKKIENLCAMGFDRNAVIVALSSKSWDVETATELLLSN*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20018
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid to express Ubc1 (E2-25K) with N-terminal 6HIS tag (contains preScission protease site); ; Estimated MW of expressed protein : 24851 Daltons