cDNA Clone
DUBA-1

Plasmid
pGEX6P-DUBA-1
Parent Plasmid
pGEX6P1
Expressed
GST-DUBA-1
Genbank
NM_017602*
Tag
GST
Species
Human
Sequence of Insert
GGATCCATGACTATACTCCCCAAAAAGAAGCCGCCGCCTCCCGACGCCGACCCCGGCGGCACGGGCGTGGGCGGCGGCGATCGCGACCGTGACTCCGGCGTCGTGGGGGCCCGTCCGCGAGCTTCGCCACCGCCTCAAGGCCCGCTACCAGGACCGCCGGGCGCTCTTCATCGCTGGGCGCTGGCCGTGCCGCCTGGTGCAGTGGCGGGTCCCCGGCCACAACAGGCTTCTCCACCTCCTTGCGGGGGCCCAGGTGGTCCCGGCGGCGGTCCCGGCGACGCGCTGGGCGCAGCGGCGGCGGGTGTG
GGTGCCGCGGGCGTGGTGGTGGGTGTGGGTGGTGCCGTAGGCGTGGGCGG
CTGCTGCTCCGGGCCTGGGCACAGCAAGCGGCGACGTCAAGCTCCCGGGG
TTGGCGCGGTTGGCGGGGGCAGTCCCGAGCGTGAGGAGGTCGGCGCAGGC
TACAACAGTGAGGACGAGTATGAGGCGGCTGCAGCACGCATCGAGGCTAT
GGACCCTGCCACTGTCGAGCAGCAGGAGCATTGGTTTGAAAAGGCCCTAC
GAGACAAGAAGGGCTTCATCATCAAGCAGATGAAGGAGGATGGCGCCTGT
CTCTTCCGGGCTGTAGCTGACCAGGTGTATGGAGACCAGGACATGCATGA
GGTTGTGCGAAAGCATTGCATGGACTATCTGATGAAGAATGCCGACTACT
TCTCCAACTATGTCACAGAGGACTTTACCACCTACATTAACAGGAAGCGG
AAAAACAATTGCCATGGCAACCACATTGAGATGCAGGCCATGGCAGAGAT
GTACAACCGTCCTGTGGAGGTGTACCAGTACAGCACAGAACCCATCAACA
CATTCCATGGGATACATCAAAACGAGGACGAACCCATTCGTGTTAGCTAC
CATCGGAATATCCACTATAATTCAGTGGTGAATCCTAACAAGGCCACCAT
TGGTGTGGGGCTGGGCCTGCCATCATTCAAACCAGGGTTTGCAGAGCAGT
CTCTGATGAAGAATGCCATAAAAACATCGGAGGAGTCATGGATTGAACAG
CAGATGCTAGAAGACAAGAAACGGGCCACAGACTGGGAGGCCACAAATGA
AGCCATCGAGGAGCAGGTGGCTCGGGAATCCTACCTGCAGTGGTTGCGGG
ATCAGGAGAAACAGGCTCGCCAGGTCCGAGGCCCCAGCCAGCCCCGGAAA
GCCAGCGCCACATGCAGTTCGGCCACAGCAGCAGCCTCCAGTGGCCTGGA
GGAGTGGACTAGCCGGTCCCCGCGGCAGCGGAGTTCAGCCTCGTCACCTG
AGCACCCTGAGCTGCATGCTGAATTGGGCATGAAGCCCCCTTCCCCAGGC
ACTGTTTTAGCTCTTGCCAAACCTCCTTCGCCCTGTGCGCCAGGTACAAG
CAGTCAGTTCTCGGCAGGGGCCGACCGGGCAACTTCCCCCCTTGTGTCCC
TCTACCCTGCTTTGGAGTGCCGGGCCCTCATTCAGCAGATGTCCCCCTCT
GCCTTTGGTCTGAATGACTGGGATGATGATGAGATCCTAGCTTCGGTGCT
GGCAGTGTCCCAACAGGAATACCTAGACAGTATGAAGAAAAACAAAGTGC
ACAGAGACCCGCCCCCAGACAAGAGTTGAGCGGCCGC
Amino Acid Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMTILPKKKPPPPDADPGGT
GVGGGDRDRDSGVVGARPRASPPPQGPLPGPPGALHRWALAVPPGAVAGP
RPQQASPPPCGGPGGPGGGPGDALGAAAAGVGAAGVVVGVGGAVGVGGCC
SGPGHSKRRRQAPGVGAVGGGSPEREEVGAGYNSEDEYEAAAARIEAMDP
ATVEQQEHWFEKALRDKKGFIIKQMKEDGACLFRAVADQVYGDQDMHEVV
RKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHGNHIEMQAMAEMYN
RPVEVYQYSTEPINTFHGIHQNEDEPIRVSYHRNIHYNSVVNPNKATIGV
GLGLPSFKPGFAEQSLMKNAIKTSEESWIEQQMLEDKKRATDWEATNEAI
EEQVARESYLQWLRDQEKQARQVRGPSQPRKASATCSSATAAASSGLEEW
TSRSPRQRSSASSPEHPELHAELGMKPPSPGTVLALAKPPSPCAPGTSSQ
FSAGADRATSPLVSLYPALECRALIQQMSPSAFGLNDWDDDEILASVLAV
SQQEYLDSMKKNKVHRDPPPDKS*


Vector Type
Bacterial
Antibiotic
Amp
DU Number
DU20014
Location
PPU
MTA
Notes
Bacterial expression construct for DUBA-1 with N-terminal GST tag (contains preScission protease site)
With reference to NM_017602 DUBA-1 has 2 deleted stretches (nt48-119 and nt910-924). Catalytic residues in this protein are Asp197, Cys200 and His305.

Estimated MW of expressed protein : 84832 Daltons
Price
£125.00
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