cDNA Clone DUBA-1

• Plasmid: pGEX6P-DUBA-1
• Parent Plasmid: pGEX6P1
• Expressed: GST-DUBA-1
• Genbank: NM_017602*
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGACTATACTCCCCAAAAAGAAGCCGCCGCCTCCCGACGCCGACCCCGGCGGCACGGGCGTGGGCGGCGGCGATCGCGACCGTGACTCCGGCGTCGTGGGGGCCCGTCCGCGAGCTTCGCCACCGCCTCAAGGCCCGCTACCAGGACCGCCGGGCGCTCTTCATCGCTGGGCGCTGGCCGTGCCGCCTGGTGCAGTGGCGGGTCCCCGGCCACAACAGGCTTCTCCACCTCCTTGCGGGGGCCCAGGTGGTCCCGGCGGCGGTCCCGGCGACGCGCTGGGCGCAGCGGCGGCGGGTGTG
GGTGCCGCGGGCGTGGTGGTGGGTGTGGGTGGTGCCGTAGGCGTGGGCGG
CTGCTGCTCCGGGCCTGGGCACAGCAAGCGGCGACGTCAAGCTCCCGGGG
TTGGCGCGGTTGGCGGGGGCAGTCCCGAGCGTGAGGAGGTCGGCGCAGGC
TACAACAGTGAGGACGAGTATGAGGCGGCTGCAGCACGCATCGAGGCTAT
GGACCCTGCCACTGTCGAGCAGCAGGAGCATTGGTTTGAAAAGGCCCTAC
GAGACAAGAAGGGCTTCATCATCAAGCAGATGAAGGAGGATGGCGCCTGT
CTCTTCCGGGCTGTAGCTGACCAGGTGTATGGAGACCAGGACATGCATGA
GGTTGTGCGAAAGCATTGCATGGACTATCTGATGAAGAATGCCGACTACT
TCTCCAACTATGTCACAGAGGACTTTACCACCTACATTAACAGGAAGCGG
AAAAACAATTGCCATGGCAACCACATTGAGATGCAGGCCATGGCAGAGAT
GTACAACCGTCCTGTGGAGGTGTACCAGTACAGCACAGAACCCATCAACA
CATTCCATGGGATACATCAAAACGAGGACGAACCCATTCGTGTTAGCTAC
CATCGGAATATCCACTATAATTCAGTGGTGAATCCTAACAAGGCCACCAT
TGGTGTGGGGCTGGGCCTGCCATCATTCAAACCAGGGTTTGCAGAGCAGT
CTCTGATGAAGAATGCCATAAAAACATCGGAGGAGTCATGGATTGAACAG
CAGATGCTAGAAGACAAGAAACGGGCCACAGACTGGGAGGCCACAAATGA
AGCCATCGAGGAGCAGGTGGCTCGGGAATCCTACCTGCAGTGGTTGCGGG
ATCAGGAGAAACAGGCTCGCCAGGTCCGAGGCCCCAGCCAGCCCCGGAAA
GCCAGCGCCACATGCAGTTCGGCCACAGCAGCAGCCTCCAGTGGCCTGGA
GGAGTGGACTAGCCGGTCCCCGCGGCAGCGGAGTTCAGCCTCGTCACCTG
AGCACCCTGAGCTGCATGCTGAATTGGGCATGAAGCCCCCTTCCCCAGGC
ACTGTTTTAGCTCTTGCCAAACCTCCTTCGCCCTGTGCGCCAGGTACAAG
CAGTCAGTTCTCGGCAGGGGCCGACCGGGCAACTTCCCCCCTTGTGTCCC
TCTACCCTGCTTTGGAGTGCCGGGCCCTCATTCAGCAGATGTCCCCCTCT
GCCTTTGGTCTGAATGACTGGGATGATGATGAGATCCTAGCTTCGGTGCT
GGCAGTGTCCCAACAGGAATACCTAGACAGTATGAAGAAAAACAAAGTGC
ACAGAGACCCGCCCCCAGACAAGAGTTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMTILPKKKPPPPDADPGGT
GVGGGDRDRDSGVVGARPRASPPPQGPLPGPPGALHRWALAVPPGAVAGP
RPQQASPPPCGGPGGPGGGPGDALGAAAAGVGAAGVVVGVGGAVGVGGCC
SGPGHSKRRRQAPGVGAVGGGSPEREEVGAGYNSEDEYEAAAARIEAMDP
ATVEQQEHWFEKALRDKKGFIIKQMKEDGACLFRAVADQVYGDQDMHEVV
RKHCMDYLMKNADYFSNYVTEDFTTYINRKRKNNCHGNHIEMQAMAEMYN
RPVEVYQYSTEPINTFHGIHQNEDEPIRVSYHRNIHYNSVVNPNKATIGV
GLGLPSFKPGFAEQSLMKNAIKTSEESWIEQQMLEDKKRATDWEATNEAI
EEQVARESYLQWLRDQEKQARQVRGPSQPRKASATCSSATAAASSGLEEW
TSRSPRQRSSASSPEHPELHAELGMKPPSPGTVLALAKPPSPCAPGTSSQ
FSAGADRATSPLVSLYPALECRALIQQMSPSAFGLNDWDDDEILASVLAV
SQQEYLDSMKKNKVHRDPPPDKS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20014
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression construct for DUBA-1 with N-terminal GST tag (contains preScission protease site); With reference to NM_017602 DUBA-1 has 2 deleted stretches (nt48-119 and nt910-924). Catalytic residues in this protein are Asp197, Cys200 and His305.; ; Estimated MW of expressed protein : 84832 Daltons