cDNA Clone Cullin3

• Plasmid: pFastbacDual DAC TEV Cullin3 (insect optimised), Rbx1
• Parent Plasmid: pFastbacDual DAC TEV
• Expressed: DAC-Cullin3 (codon optimised insect)
• Tag: DAC
• Species: Human

Sequence of Insert

GGATCCATGTCTAACCTGTCTAAGGGCACTGGCAGCCGTAAGGATACCAAGATGAGAATCAGAGCCTTCCCGATGACTATGGACGAAAAGTACGTGAACTCCATCTGGGACCTGCTCAAGAACGCTATCCAGGAGATCCAAAGGAAGAACAACTCCGGCTTGTCTTTCGAGGAACTGTACAGAAACGCCTACACTATGGTGCTGCACAAGCACGGCGAGAAGCTCTACACTGGCTTGCGTGAAGTGGTCACAGAGCACCTCATCAACAAGGTTCGCGAAGATGTGCTCAACTCTTTGAACAACAACTTCCTGCAGACTCTCAACCAGGCTTGGAACGACCACCAGACAGCTATGGTCATGATCAGGGATATCCTGATGTACATGGACAGAGTCTACGTTCAGCAAAACAACGTGGAGAACGTCTACAACCTCGGATTGATCATCTTCCGTGACCAGGTTGTGCGCTACGGTTGCATCCGTGATCACCTCCGCCAAACCTTGCTGGACATGATCGCTCGTGAACGCAAGGGAGAGGTCGTTGATAGGGGTGCTATCAGAAACGCCTGTCAGATGCTGATGATCCTGGGTCTCGAAGGCAGGAGCGTCTACGAGGAAGACTTCGAGGCTCCCTTCCTCGAAATGTCGGCCGAGTTCTTCCAGATGGAATCCCAAAAGTTCTTGGCTGAGAACAGCGCCTCAGTCTACATCAAGAAGGTTGAAGCCCGTATCAACGAGGAAATCGAGCGCGTTATGCACTGCCTGGACAAGTCAACCGAGGAACCTATCGTCAAGGTGGTCGAGCGCGAACTCATCTCCAAGCACATGAAGACCATCGTCGAGATGGAAAACTCTGGTCTGGTTCACATGCTCAAGAACGGCAAGACTGAGGACCTGGGATGTATGTACAAGCTCTTCTCCAGGGTGCCCAACGGCCTGAAGACTATGTGCGAATGTATGTCCTCTTACCTCAGAGAGCAGGGAAAGGCTTTGGTTTCTGAGGAAGGCGAGGGAAAGAACCCTGTGGACTACATCCAAGGCCTCTTGGATTTGAAGTCTAGGTTCGACCGTTTCCTGCTGGAGTCCTTCAACAACGATAGGCTGTTCAAGCAGACCATCGCCGGAGACTTCGAGTACTTCTTGAACCTGAACTCCCGTTCCCCTGAATACCTCAGCTTGTTCATCGACGATAAGCTGAAGAAGGGAGTGAAGGGTCTCACCGAGCAAGAAGTCGAGACTATCTTGGATAAGGCTATGGTCCTGTTCCGTTTCATGCAGGAAAAGGACGTTTTCGAGCGCTACTACAAGCAACACCTGGCCCGTCGCTTGCTGACTAACAAGTCTGTGAGCGACGATAGCGAAAAGAACATGATCTCAAAGTTGAAGACCGAGTGCGGTTGTCAGTTCACTTCTAAGCTGGAAGGCATGTTCCGTGATATGTCAATCTCGAACACCACTATGGACGAGTTCCGCCAGCACCTGCAAGCTACCGGTGTGTCACTCGGTGGCGTGGACCTGACTGTGCGTGTCCTGACAACCGGCTACTGGCCCACTCAGTCGGCTACACCTAAGTGCAACATCCCCCCTGCTCCACGTCACGCCTTCGAAATCTTCAGGAGATTCTACTTGGCCAAGCACAGCGGACGCCAGCTGACACTCCAACACCACATGGGTTCAGCTGATCTGAACGCCACCTTCTACGGCCCGGTGAAGAAGGAAGACGGCTCCGAGGTTGGAGTGGGAGGTGCTCAGGTGACCGGTTCAAACACTCGCAAGCACATCCTGCAGGTCTCGACATTCCAAATGACCATCTTGATGCTGTTCAACAACAGGGAGAAGTACACATTCGAGGAAATCCAGCAAGAAACCGACATCCCAGAACGTGAGCTGGTGCGTGCTCTGCAGTCGCTGGCTTGCGGAAAGCCAACTCAACGCGTCTTGACAAAGGAGCCGAAGTCCAAGGAAATCGAGAACGGTCACATCTTCACAGTGAACGATCAGTTCACCTCTAAGCTGCACCGTGTTAAGATCCAGACTGTGGCTGCCAAGCAAGGCGAGAGCGACCCCGAAAGGAAGGAGACAAGACAGAAGGTGGACGATGACCGCAAGCACGAAATCGAGGCTGCCATCGTCAGGATCATGAAGAGCAGAAAGAAGATGCAACACAACGTCCTCGTTGCTGAGGTCACCCAGCAACTCAAGGCCAGGTTCTTGCCATCACCGGTTGTGATCAAGAAGAGAATCGAGGGTCTGATCGAGAGAGAATACTTGGCTAGGACTCCAGAGGACAGAAAGGTTTACACTTACGTTGCTTAAGCGGCCGC

Amino Acid Sequence

MSAIPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKLLTWGFRFFETVNPENLYFQGGSMSNLSKGTGSRKDTKMRIRAFPMTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKHGEKLYTGLREVVTEHLINKVREDVLNSLNNNFLQTLNQAWNDHQTAMVMIRDILMYMDRVYVQQNNVENVYNLGLIIFRDQVVRYGCIRDHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYEEDFEAPFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERVMHCLDKSTEEPIVKVVERELISKHMKTIVEMENSGLVHMLKNGKTEDLGCMYKLFSRVPNGLKTMCECMSSYLREQGKALVSEEGEGKNPVDYIQGLLDLKSRFDRFLLESFNNDRLFKQTIAGDFEYFLNLNSRSPEYLSLFIDDKLKKGVKGLTEQEVETILDKAMVLFRFMQEKDVFERYYKQHLARRLLTNKSVSDDSEKNMISKLKTECGCQFTSKLEGMFRDMSISNTTMDEFRQHLQATGVSLGGVDLTVRVLTTGYWPTQSATPKCNIPPAPRHAFEIFRRFYLAKHSGRQLTLQHHMGSADLNATFYGPVKKEDGSEVGVGGAQVTGSNTRKHILQVSTFQMTILMLFNNREKYTFEEIQQETDIPERELVRALQSLACGKPTQRVLTKEPKSKEIENGHIFTVNDQFTSKLHRVKIQTVAAKQGESDPERKETRQKVDDDRKHEIEAAIVRIMKSRKKMQHNVLVAEVTQQLKARFLPSPVVIKKRIEGLIEREYLARTPEDRKVYTYVA*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU23291
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 21851