cDNA Clone Cullin3

• Plasmid: pETDuet-6His_TEV-Rbx1, GST_TEV-Cullin3 delta 403-459 (codon optimised bacterial)
• Parent Plasmid: pETDuet1
• Expressed: GST-Cullin3 delta 403-459
• Tag: GST
• Species: Human

Sequence of Insert

gtcgacatgtcccctatactaggttattggaaaattaagggccttgtgcaacccactcgacttcttttggaatatcttgaagaaaaatatgaagagcatttgtatgagcgcgatgaaggtgataaatggcgaaacaaaaagtttgaattgggtttggagtttcccaatcttccttattatattgatggtgatgttaaattaacacagtctatggccatcatacgttatatagctgacaagcacaacatgttgggtggttgtccaaaagagcgtgcagagatttcaatgcttgaaggagcggttttggatattagatacggtgtttcgagaattgcatatagtaaagactttgaaactctcaaagttgattttcttagcaagctacctgaaatgctgaaaatgttcgaagatcgtttatgtcataaaacatatttaaatggtgatcatgtaacccatcctgacttcatgttgtatgacgctcttgatgttgttttatacatggacccaatgtgcctggatgcgttcccaaaattagtttgttttaaaaaacgtattgaagctatcccacaaattgataagtacttgaaatccagcaagtatatagcatggcctttgcagggctggcaagccacgtttggtggtggcgaccatcctccaaaatcggatggatccGAAAACCTGTATTTTCAGATGAGCAATCTGAGCAAAGGCACCGGTTCCCGTAAAGACACCAAAATGCGTATCCGTGCGTTCCCGATGACGATGGACGAAAAATATGTGAACAGCATTTGGGATCTGCTGAAAAACGCGATTCAGGAAATCCAACGTAAAAACAATTCAGGTCTGTCGTTTGAAGAACTGTATCGCAATGCCTACACGATGGTGCTGCATAAACACGGCGAAAAACTGTATACCGGTCTGCGTGAAGTGGTTACGGAACATCTGATTAATAAAGTCCGCGAAGACGTGCTGAACAGCCTGAACAATAACTTTCTGCAGACCCTGAATCAAGCATGGAACGATCACCAGACGGCTATGGTGATGATTCGTGACATCCTGATGTATATGGATCGCGTTTACGTCCAGCAAAATAACGTGGAAAATGTTTATAACCTGGGTCTGATTATCTTCCGTGATCAGGTCGTGCGCTACGGCTGCATCCGTGACCATCTGCGCCAAACCCTGCTGGATATGATTGCCCGTGAACGCAAAGGCGAAGTTGTGGATCGTGGTGCGATCCGCAACGCCTGTCAGATGCTGATGATTCTGGGCCTGGAAGGTCGTTCAGTGTATGAAGAAGATTTCGAAGCGCCGTTTCTGGAAATGAGTGCCGAATTTTTCCAGATGGAATCCCAAAAATTTCTGGCAGAAAATAGTGCTTCCGTTTACATCAAAAAAGTCGAAGCACGTATTAACGAAGAAATCGAACGCGTCATGCACTGCCTGGATAAAAGCACCGAAGAACCGATTGTTAAAGTGGTTGAACGCGAACTGATCAGTAAACACATGAAAACCATCGTTGAAATGGAAAATTCCGGCCTGGTCCACATGCTGAAAAACGGCAAAACCGAAGACCTGGGTTGTATGTATAAACTGTTTAGTCGTGTGCCGAATGGTCTGAAAACCATGTGCGAATGTATGAGCAGCTACCTGCGCGAACAGGGCAAAGCACTGGTCTCCGAAGAAGGCGAAGGTAAAAACCCGGTGGATTATATCCAGGGTCTGCTGGACCTGAAATCACGTTTTGATCGCTTCCTGCTGGAATCGTTCAATAACGACCGTCTGTTTAAACAGACCATTGCGGGCGATTTTGAATATTTCCTGAATCTGAACTCACGCTCGCCGGAATACCTGAGTCTGTTTATCGATGACAAACTGAAAAAAGGCGTGAAAGGTACCGAATGCGGCTGTCAGTTCACGAGCAAACTGGAAGGCATGTTTCGTGACATGAGTATTTCCAATACCACGATGGATGAATTTCGCCAGCACCTGCAAGCGACCGGTGTGTCACTGGGCGGTGTTGATCTGACGGTGCGTGTTCTGACCACGGGTTATTGGCCGACCCAGTCGGCAACGCCGAAATGCAACATCCCGCCGGCACCGCGTCATGCATTTGAAATTTTCCGTCGCTTTTATCTGGCCAAACACTCAGGCCGCCAGCTGACCCTGCAACATCACATGGGTTCGGCAGACCTGAATGCTACGTTCTACGGCCCGGTGAAAAAAGAAGATGGCAGCGAAGTCGGTGTGGGCGGTGCCCAGGTGACCGGTAGCAACACGCGTAAACATATCCTGCAGGTTTCTACCTTCCAAATGACGATTCTGATGCTGTTTAATAACCGCGAAAAATACACCTTCGAAGAAATCCAGCAAGAAACGGATATTCCGGAACGTGAACTGGTGCGCGCGCTGCAGAGCCTGGCATGTGGTAAACCGACCCAACGCGTTCTGACGAAAGAACCGAAATCTAAAGAAATCGAAAACGGCCATATCTTCACCGTGAACGACCAGTTTACGAGTAAACTGCACCGTGTCAAAATCCAGACCGTGGCGGCCAAACAGGGTGAATCCGATCCGGAACGTAAAGAAACGCGCCAGAAAGTGGATGACGATCGCAAACATGAAATTGAAGCAGCTATTGTTCGTATCATGAAAAGCCGTAAGAAAATGCAGCACAATGTTCTGGTCGCAGAAGTTACCCAGCAACTGAAAGCTCGTTTTCTGCCGTCTCCGGTCGTGATTAAAAAACGCATCGAAGGCCTGATTGAACGTGAATACCTGGCTCGCACCCCGGAAGATCGTAAAGTCTACACCTATGTCGCCTGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDGSENLYFQMSNLSKGTGSRKDTKMRIRAFPMTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKHGEKLYTGLREVVTEHLINKVREDVLNSLNNNFLQTLNQAWNDHQTAMVMIRDILMYMDRVYVQQNNVENVYNLGLIIFRDQVVRYGCIRDHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYEEDFEAPFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERVMHCLDKSTEEPIVKVVERELISKHMKTIVEMENSGLVHMLKNGKTEDLGCMYKLFSRVPNGLKTMCECMSSYLREQGKALVSEEGEGKNPVDYIQGLLDLKSRFDRFLLESFNNDRLFKQTIAGDFEYFLNLNSRSPEYLSLFIDDKLKKGVKGTECGCQFTSKLEGMFRDMSISNTTMDEFRQHLQATGVSLGGVDLTVRVLTTGYWPTQSATPKCNIPPAPRHAFEIFRRFYLAKHSGRQLTLQHHMGSADLNATFYGPVKKEDGSEVGVGGAQVTGSNTRKHILQVSTFQMTILMLFNNREKYTFEEIQQETDIPERELVRALQSLACGKPTQRVLTKEPKSKEIENGHIFTVNDQFTSKLHRVKIQTVAAKQGESDPERKETRQKVDDDRKHEIEAAIVRIMKSRKKMQHNVLVAEVTQQLKARFLPSPVVIKKRIEGLIEREYLARTPEDRKVYTYVA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU23254
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: both genes optimised for expression in E. coli, both genes preceded by Blunt TEV (ENLYFQ straight into Met)