Plasmid
pETDuet-6His_TEV-Rbx1, GST_TEV-Cullin3 (codon optimised bacterial)
Parent Plasmid
pETDuet1
Expressed
GST-Cullin3
Tag
GST
Sequence of Insert
View Sequence
gtcgacatgtcccctatactaggttattggaaaattaagggccttgtgcaacccactcgacttcttttggaatatcttgaagaaaaatatgaagagcatttgtatgagcgcgatgaaggtgataaatggcgaaacaaaaagtttgaattgggtttggagtttcccaatcttccttattatattgatggtgatgttaaattaacacagtctatggccatcatacgttatatagctgacaagcacaacatgttgggtggttgtccaaaagagcgtgcagagatttcaatgcttgaaggagcggttttggatattagatacggtgtttcgagaattgcatatagtaaagactttgaaactctcaaagttgattttcttagcaagctacctgaaatgctgaaaatgttcgaagatcgtttatgtcataaaacatatttaaatggtgatcatgtaacccatcctgacttcatgttgtatgacgctcttgatgttgttttatacatggacccaatgtgcctggatgcgttcccaaaattagtttgttttaaaaaacgtattgaagctatcccacaaattgataagtacttgaaatccagcaagtatatagcatggcctttgcagggctggcaagccacgtttggtggtggcgaccatcctccaaaatcggatggatccGAAAACCTGTATTTTCAGATGAGCAATCTGAGCAAAGGCACCGGTTCCCGTAAAGACACCAAAATGCGTATCCGTGCGTTCCCGATGACGATGGACGAAAAATATGTGAACAGCATTTGGGATCTGCTGAAAAACGCGATTCAGGAAATCCAACGTAAAAACAATTCAGGTCTGTCGTTTGAAGAACTGTATCGCAATGCCTACACGATGGTGCTGCATAAACACGGCGAAAAACTGTATACCGGTCTGCGTGAAGTGGTTACGGAACATCTGATTAATAAAGTCCGCGAAGACGTGCTGAACAGCCTGAACAATAACTTTCTGCAGACCCTGAATCAAGCATGGAACGATCACCAGACGGCTATGGTGATGATTCGTGACATCCTGATGTATATGGATCGCGTTTACGTCCAGCAAAATAACGTGGAAAATGTTTATAACCTGGGTCTGATTATCTTCCGTGATCAGGTCGTGCGCTACGGCTGCATCCGTGACCATCTGCGCCAAACCCTGCTGGATATGATTGCCCGTGAACGCAAAGGCGAAGTTGTGGATCGTGGTGCGATCCGCAACGCCTGTCAGATGCTGATGATTCTGGGCCTGGAAGGTCGTTCAGTGTATGAAGAAGATTTCGAAGCGCCGTTTCTGGAAATGAGTGCCGAATTTTTCCAGATGGAATCCCAAAAATTTCTGGCAGAAAATAGTGCTTCCGTTTACATCAAAAAAGTCGAAGCACGTATTAACGAAGAAATCGAACGCGTCATGCACTGCCTGGATAAAAGCACCGAAGAACCGATTGTTAAAGTGGTTGAACGCGAACTGATCAGTAAACACATGAAAACCATCGTTGAAATGGAAAATTCCGGCCTGGTCCACATGCTGAAAAACGGCAAAACCGAAGACCTGGGTTGTATGTATAAACTGTTTAGTCGTGTGCCGAATGGTCTGAAAACCATGTGCGAATGTATGAGCAGCTACCTGCGCGAACAGGGCAAAGCACTGGTCTCCGAAGAAGGCGAAGGTAAAAACCCGGTGGATTATATCCAGGGTCTGCTGGACCTGAAATCACGTTTTGATCGCTTCCTGCTGGAATCGTTCAATAACGACCGTCTGTTTAAACAGACCATTGCGGGCGATTTTGAATATTTCCTGAATCTGAACTCACGCTCGCCGGAATACCTGAGTCTGTTTATCGATGACAAACTGAAAAAAGGCGTGAAAGGTCTGACCGAACAGGAAGTTGAAACGATTCTGGACAAAGCAATGGTTCTGTTTCGTTTCATGCAGGAAAAAGATGTCTTCGAACGCTATTACAAACAACATCTGGCTCGTCGCCTGCTGACCAATAAAAGCGTGTCTGATGACAGCGAGAAAAACATGATCTCTAAACTGAAAACCGAATGCGGCTGTCAGTTCACGAGCAAACTGGAAGGCATGTTTCGTGACATGAGTATTTCCAATACCACGATGGATGAATTTCGCCAGCACCTGCAAGCGACCGGTGTGTCACTGGGCGGTGTTGATCTGACGGTGCGTGTTCTGACCACGGGTTATTGGCCGACCCAGTCGGCAACGCCGAAATGCAACATCCCGCCGGCACCGCGTCATGCATTTGAAATTTTCCGTCGCTTTTATCTGGCCAAACACTCAGGCCGCCAGCTGACCCTGCAACATCACATGGGTTCGGCAGACCTGAATGCTACGTTCTACGGCCCGGTGAAAAAAGAAGATGGCAGCGAAGTCGGTGTGGGCGGTGCCCAGGTGACCGGTAGCAACACGCGTAAACATATCCTGCAGGTTTCTACCTTCCAAATGACGATTCTGATGCTGTTTAATAACCGCGAAAAATACACCTTCGAAGAAATCCAGCAAGAAACGGATATTCCGGAACGTGAACTGGTGCGCGCGCTGCAGAGCCTGGCATGTGGTAAACCGACCCAACGCGTTCTGACGAAAGAACCGAAATCTAAAGAAATCGAAAACGGCCATATCTTCACCGTGAACGACCAGTTTACGAGTAAACTGCACCGTGTCAAAATCCAGACCGTGGCGGCCAAACAGGGTGAATCCGATCCGGAACGTAAAGAAACGCGCCAGAAAGTGGATGACGATCGCAAACATGAAATTGAAGCAGCTATTGTTCGTATCATGAAAAGCCGTAAGAAAATGCAGCACAATGTTCTGGTCGCAGAAGTTACCCAGCAACTGAAAGCTCGTTTTCTGCCGTCTCCGGTCGTGATTAAAAAACGCATCGAAGGCCTGATTGAACGTGAATACCTGGCTCGCACCCCGGAAGATCGTAAAGTCTACACCTATGTCGCCTGAgcggccgc
Amino Acid Sequence
View Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDGSENLYFQMSNLSKGTGSRKDTKMRIRAFPMTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKHGEKLYTGLREVVTEHLINKVREDVLNSLNNNFLQTLNQAWNDHQTAMVMIRDILMYMDRVYVQQNNVENVYNLGLIIFRDQVVRYGCIRDHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYEEDFEAPFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERVMHCLDKSTEEPIVKVVERELISKHMKTIVEMENSGLVHMLKNGKTEDLGCMYKLFSRVPNGLKTMCECMSSYLREQGKALVSEEGEGKNPVDYIQGLLDLKSRFDRFLLESFNNDRLFKQTIAGDFEYFLNLNSRSPEYLSLFIDDKLKKGVKGLTEQEVETILDKAMVLFRFMQEKDVFERYYKQHLARRLLTNKSVSDDSEKNMISKLKTECGCQFTSKLEGMFRDMSISNTTMDEFRQHLQATGVSLGGVDLTVRVLTTGYWPTQSATPKCNIPPAPRHAFEIFRRFYLAKHSGRQLTLQHHMGSADLNATFYGPVKKEDGSEVGVGGAQVTGSNTRKHILQVSTFQMTILMLFNNREKYTFEEIQQETDIPERELVRALQSLACGKPTQRVLTKEPKSKEIENGHIFTVNDQFTSKLHRVKIQTVAAKQGESDPERKETRQKVDDDRKHEIEAAIVRIMKSRKKMQHNVLVAEVTQQLKARFLPSPVVIKKRIEGLIEREYLARTPEDRKVYTYVA*
Vector Type
Bacterial
Antibiotic
Amp
DU Number
DU23253
Unit Source
Location
PPU
MTA
Notes
both genes optimised for expression in E. coli, both genes preceded by Blunt TEV (ENLYFQ straight into Met)
Price
£125.00
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