cDNA Clone USP22

• Plasmid: pFB Dual DAC-TEV USP22 C185S
• Parent Plasmid: pFastbacDual DAC TEV
• Expressed: DAC TEV USP22 C185S
• Genbank: NM_015276.1
• Tag: DAC

Sequence of Insert

ATGGTGTCCCGGCCAGAGCCCGAGGGCGAGGCCATGGACGCCGAGCTGGCGGTAGCGCCGCCGGGCTGCTCGCACCTGGGCAGCTTCAAGGTGGACAACTGGAAGCAGAACCTGCGGGCCATCTACCAGTGCTTCGTGTGGAGCGGCACGGCTGAGGCCCGCAAGCGCAAGGCCAAGTCCTGTATCTGCCATGTCTGTGGCGTCCACCTCAACAGGCTGCATTCCTGCCTCTACTGTGTCTTCTTCGGCTGTTTCACAAAGAAGCATATTCACGAGCATGCGAAGGCGAAGCGGCACAACCTGGCCATTGATCTGATGTACGGAGGCATCTACTGTTTTCTGTGCCAGGACTACATCTATGACAAAGACATGGAAATAATCGCCAAGGAGGAGCAGCGAAAAGCTTGGAAAATGCAAGGCGTTGGAGAGAAGTTTTCAACTTGGGAACCAACCAAACGGGAGCTTGAACTGCTGAAGCACAACCCGAAAAGGAGAAAGATCACCTCGAACTGCACCATAGGTCTGCGTGGGCTGATCAACCTTGGGAACACATcCTTCATGAACTGCATCGTGCAGGCCCTGACCCACACGCCACTTCTGCGGGACTTCTTCCTGTCTGACAGGCACCGCTGTGAGATGCAGAGCCCCAGCTCCTGTCTGGTCTGTGAGATGTCCTCACTGTTTCAGGAGTTTTACTCTGGACACCGGTCCCCTCACATCCCGTATAAGTTGCTGCACCTGGTGTGGACCCACGCGAGGCACCTAGCAGGCTACGAGCAGCAGGACGCCCACGAGTTCCTCATCGCGGCCCTGGACGTGCTCCACCGACACTGCAAAGGTGATGACAATGGGAAGAAGGCCAACAACCCCAACCACTGCAACTGCATCATAGACCAGATCTTCACAGGCGGGTTGCAGTCAGACGTCACCTGCCAAGTCTGCCATGGAGTCTCCACCACCATCGACCCCTTCTGGGACATCAGCTTGGATCTCCCCGGCTCTTCCACCCCATTCTGGCCCCTGAGCCCAGGGAGCGAGGGCAACGTGGTAAACGGGGAAAGCCACGTGTCGGGAACCACCACGCTCACGGACTGCCTGCGACGATTCACCAGACCAGAGCACTTGGGCAGCAGCGCCAAGATCAAGTGCAGCGGTTGCCATAGCTACCAGGAGTCCACAAAGCAGCTCACTATGAAGAAACTGCCCATCGTAGCCTGTTTTCATCTCAAACGATTTGAACACTCAGCCAAGCTGCGGCGGAAGATCACCACGTATGTGTCCTTCCCCCTGGAGCTGGACATGACCCCTTTCATGGCCTCCAGCAAAGAGAGCAGGATGAATGGACAGTACCAGCAGCCCACGGACAGTCTCAACAATGACAACAAGTATTCCCTGTTTGCTGTTGTTAACCATCAAGGGACCTTGGAGAGTGGCCACTACACCAGCTTTATCCGGCAGCACAAAGACCAGTGGTTCAAGTGTGACGATGCCATCATCACCAAGGCCAGCATCAAGGACGTCCTGGACAGCGAAGGGTACTTGCTGTTCTATCACAAACAGTTCCTGGAATACGAGTAG

Amino Acid Sequence

LEMSVPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKLLTWGFRFFETVNPENLYFQGEFMVSRPEPEGEAMDAELAVAPPGCSHLGSFKVDNWKQNLRAIYQCFVWSGTAEARKRKAKSCICHVCGVHLNRLHSCLYCVFFGCFTKKHIHEHAKAKRHNLAIDLMYGGIYCFLCQDYIYDKDMEIIAKEEQRKAWKMQGVGEKFSTWEPTKRELELLKHNPKRRKITSNCTIGLRGLINLGNTSFMNCIVQALTHTPLLRDFFLSDRHRCEMQSPSSCLVCEMSSLFQEFYSGHRSPHIPYKLLHLVWTHARHLAGYEQQDAHEFLIAALDVLHRHCKGDDNGKKANNPNHCNCIIDQIFTGGLQSDVTCQVCHGVSTTIDPFWDISLDLPGSSTPFWPLSPGSEGNVVNGESHVSGTTTLTDCLRRFTRPEHLGSSAKIKCSGCHSYQESTKQLTMKKLPIVACFHLKRFEHSAKLRRKITTYVSFPLELDMTPFMASSKESRMNGQYQQPTDSLNNDNKYSLFAVVNHQGTLESGHYTSFIRQHKDQWFKCDDAIITKASIKDVLDSEGYLLFYHKQFLEYE*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU22252
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 2 silent mutations t270c c321t