cDNA Clone Fam83g

• Plasmid: pBabepuro Fam83G P81T
• Parent Plasmid: pBabe puro
• Expressed: Fam83G P81T
• Genbank: NM_001039999.2
• Species: Human

Sequence of Insert

ATGGCCTTCTCTCAGGTGCAGTGTCTGGACGACAACCATGTGAACTGGCGCTCCAGCGAGTCCAAGCCTGAGTTCTTCTACAGCGAGGAGCAGCGGCTGGCGCTGGAGGCCCTGGTGGCCCGCGGCCGGGACGCCTTCTACGAGGTGCTCAAGCGGGAGAACATCCGAGACTTCCTCTCGGAGCTGGAGCTCAAGCGCATCCTGGAGACCATCGAGGTGTACGACCCGGGCTCTGAGGACACTCGGGGCACGGGCCCCTCTCAGGGGCCCGAGGACAATGGGGTCGGCGACGGCGAGGAAGCCAGCGGGGCGGATGGGGTCCCCATCGAGGCCGAGCCGCTGCCCTCCCTGGAGTACTGGCCCCAGAAGTCGGACCGCTCCATCCCGCAGCTGGACCTGGGCTGGCCCGACACCATCGCCTACCGCGGCGTGACCCGGGCTAGCGTCTACATGCAGCCCCCCATAGACGGGCAGGCCCACATCAAAGAGGTGGTGCGGAAGATGATCAGCCAGGCACAGAAGGTGATAGCTGTGGTCATGGACATGTTCACCGACGTGGACATCTTCAAGGACCTGCTGGACGCCGGCTTCAAGAGGAAAGTGGCCGTGTACATCATCGTGGATGAGAGTAACGTCAAGTACTTCCTGCACATGTGTGAGCGGGCCTGCATGCACCTGGGGCACCTCAAGAATCTCAGAGTGCGGAGCAGCGGGGGAACTGAGTTCTTCACGCGGTCGGCAACCAAGTTCAAGGGTGCCCTGGCCCAGAAGTTCATGTTTGTGGATGGAGACCGGGCTGTGTGCGGCTCCTACAGCTTCACGTGGTCGGCCGCGCGGACGGACCGGAATGTGATCTCTGTGCTGTCTGGCCAGGTGGTGGAGATGTTTGACCGGCAGTTCCAGGAGCTGTACCTCATGTCACACAGTGTGAGCCTCAAGGGCATCCCTATGGAGAAGGAACCGGAGCCGGAGCCTATTGTGCTGCCCTCTGTGGTCCCCCTGGTGCCCGCGGGCACTGTGGCCAAGAAGCTCGTCAACCCCAAGTACGCACTTGTCAAGGCCAAGAGCGTCGACGAGATTGCCAAGATCTCCTCTGAGAAGCAGGAGGCCAAGAAGCCCCTGGGGCTGAAAGGCCCAGCGCTGGCTGAGCATCCAGGGGAACTCCCCGAGCTGCTGCCACCCATCCACCCAGGACTGCTTCACCTGGAGAGGGCCAACATGTTTGAGTACCTGCCCACGTGGGTGGAGCCAGACCCGGAGCCTGGCAGCGACATCCTGGGCTACATCAATATCATCGACCCCAACATCTGGAACCCCCAGCCCAGCCAGATGAACCGCATCAAGATCCGTGACACCTCCCAGGCCAGCGCCCAGCACCAGCTGTGGAAGCAGAGCCAGGACAGCAGGCCCCGTCCAGAGCCTTGCCCTCCCCCAGAGCCCAGTGCCCCCCAGGACGGTGTCCCAGCTGAGAACGGCCTCCCCCAGGGGGACCCTGAGCCATTGCCCCCCGTGCCCAAGCCCCGGACAGTCCCTGTGGCAGATGTACTAGCCCGGGACAGCAGTGATATTGGCTGGGTCCTGGAGCTCCCCAAAGAGGAAGCTCCCCAGAATGGGACAGACCATAGGCTACCCAGGATGGCAGGCCCAGGCCACGCCCCACTCCAGCGGCAGCTATCTGTGACCCAGGATGACCCCGAGAGCCTCGGGGTGGGGCTCCCCAATGGGCTGGATGGGGTGGAAGAAGAAGATGATGACGACTACGTAACCCTCAGTGACCAGGACAGCCACTCAGGCAGCTCCGGCCGTGGCCCTGGCCCCCGACGGCCCTCAGTGGCTTCCTCTGTGTCAGAGGAGTACTTCGAGGTGAGAGAGCACTCAGTCCCTCTCCGGAGGCGCCACTCAGAGCAAGTGGCCAACGGGCCAACCCCACCACCGCGCCGGCAGCTGAGTGCCCCCCATATAACCCGAGGGACCTTTGTTGGACCCCAGGGTGGCTCCCCATGGGCCCAGAGTCGGGGAAGAGAAGAAGCAGATGCGTTGAAGAGGATGCAGGCCCAGCGCTCCACAGACAAGGAGGCACAGGGCCAGCAGTTTCATCATCACAGGGTCCCTGCCTCAGGGACTAGGGATAAAGACGGCTTCCCAGGACCCCCTAGGTACCGCTCTGCTGCTGACAGCGTCCAGAGCTCTACCAGAAACGCTGGCCCAGCCATGGCTGGCCCCCACCACTGGCAGGCCAAGGGAGGTCAGGTACCCCGCCTGCTTCCGGACCCCGGCAGCCCAAGACTGGCCCAAAATGCCCGCCCCATGACCGATGGCAGGGCCACCGAGGAGCATCCGAGTCCCTTCGGAATCCCATACTCCAAACTGTCTCAGTCGAAGCACCTAAAGGCCAGGACGGGCGGTAGCCAGTGGGCCTCATCGGATTCTAAACGGAGGGCTCAAGCCCCCCGGGACCGCAAAGACCCCTAG

Amino Acid Sequence

MAFSQVQCLDDNHVNWRSSESKPEFFYSEEQRLALEALVARGRDAFYEVLKRENIRDFLSELELKRILETIEVYDPGSEDTRGTGPSQGPEDNGVGDGEEASGADGVPIEAEPLPSLEYWPQKSDRSIPQLDLGWPDTIAYRGVTRASVYMQPPIDGQAHIKEVVRKMISQAQKVIAVVMDMFTDVDIFKDLLDAGFKRKVAVYIIVDESNVKYFLHMCERACMHLGHLKNLRVRSSGGTEFFTRSATKFKGALAQKFMFVDGDRAVCGSYSFTWSAARTDRNVISVLSGQVVEMFDRQFQELYLMSHSVSLKGIPMEKEPEPEPIVLPSVVPLVPAGTVAKKLVNPKYALVKAKSVDEIAKISSEKQEAKKPLGLKGPALAEHPGELPELLPPIHPGLLHLERANMFEYLPTWVEPDPEPGSDILGYINIIDPNIWNPQPSQMNRIKIRDTSQASAQHQLWKQSQDSRPRPEPCPPPEPSAPQDGVPAENGLPQGDPEPLPPVPKPRTVPVADVLARDSSDIGWVLELPKEEAPQNGTDHRLPRMAGPGHAPLQRQLSVTQDDPESLGVGLPNGLDGVEEEDDDDYVTLSDQDSHSGSSGRGPGPRRPSVASSVSEEYFEVREHSVPLRRRHSEQVANGPTPPPRRQLSAPHITRGTFVGPQGGSPWAQSRGREEADALKRMQAQRSTDKEAQGQQFHHHRVPASGTRDKDGFPGPPRYRSAADSVQSSTRNAGPAMAGPHHWQAKGGQVPRLLPDPGSPRLAQNARPMTDGRATEEHPSPFGIPYSKLSQSKHLKARTGGSQWASSDSKRRAQAPRDRKDP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU42524
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: point mutant P81T from wildtype DU33460; ; BamHI is knocked out as in 33460