cDNA Clone TNPO1

• Plasmid: pcDNA5 FRT/TO Myc-TNPOI WBL
• Parent Plasmid: pcDNA5 FRT/TO
• Expressed: Myc-TNPO1 WBL
• Genbank: NCBI Gene ID: 3842
• Tag: Myc
• Species: Both Human

Sequence of Insert

ggatccatggaacaaaaattaatctcagaagaagacttgatggtgtggga
ccggcaaaccaagatggagtatgagtggaaacctgacgagcaagggcttc
agcaaatcctgcagctgttgaaggagtcccagtccccagacaccaccatc
cagagaaccgtgcaacaaaaactggaacaacttaatcagtatccagactt
taacaactacttgatttttgttcttacaaaattaaaatctgaagatgaac
ccacaagatcattgagtggtcttatcttgaagaataatgtgaaagcacac
tttcagaacttcccaaatggtgtaacagactttattaaaagtgaatgttt
aaataatattggtgactcctctcctctgattagagccactgttggtattt
tgatcacaactatagcctccaagggagaattgcagaattggcctgacctc
ttaccaaaactctgtagcctgttggattctgaagattataatacctgtga
gggagcatttggtgcccttcagaagatttgtgaagattctgctgagattt
tagacagtgatgttttagatcgtcctctcaacatcatgattcccaaattt
ttacagttcttcaagcatagtagtccaaaaataaggtctcacgctgttgc
atgtgtcaatcagtttatcatcagtaggactcaagctctaatgttgcaca
ttgattcttttattgagaatctctttgcattagctggtgatgaagaacca
gaggtacggaaaaatgtgtgccgagcacttgtgatgttgctcgaagttcg
aatggatcgcctgcttcctcacatgcataatatagttgagtacatgctac
agaggactcaagatcaagatgaaaatgtggctttagaagcctgtgaattt
tggctaactttagctgaacagccaatatgcaaagatgtactcgtaaggca
tcttcctaagttgattcctgtgttagtgaatggcatgaagtactcagaca
tagatattatcctacttaagggtgatgttgaagaagacgaaacgattcct
gatagtgaacaggatatacggccacgttttcaccgatcgaggacggtggc
tcagcagcatgatgaagatggaattgaagaggaagatgatgatgatgatg
aaattgatgatgatgatacaatttctgactggaatctaagaaaatgttct
gctgctgccctggatgttcttgcaaatgtgtatcgtgatgaactgctgcc
acatattttgccccttttgaaagaattactttttcatcatgaatgggttg
ttaaagaatcaggcattttggttttaggagcaattgctgaaggttgcatg
cagggcatgattccatacttgcctgagcttattcctcaccttattcagtg
cctctctgataaaaaggctcttgtgcgttccataacatgctggactctta
gccgctatgcacactgggtggtcagccagccgccagacacgtacctgaag
ccattaatgacagaattgctaaagcgcatcctggacagcaacaagagagt
acaagaagctgcctgcagtgcctttgctaccctagaagaggaggcttgta
cagaacttgttccttaccttgcttatatacttgataccctggtctttgca
tttagtaaataccagcataagaacctgctcattctttacgatgccatagg
aacattagcagattcagtaggacatcatttaaacaaaccagaatatattc
agatgctaatgcctccactgatccagaaatggaacatgttaaaggatgaa
gataaagatctcttccctttacttgagtgcctatcttcagttgccacagc
actgcagtctggattccttccgtactgtgaacctgtgtatcagcgttgtg
taaacctagtacagaagactcttgcacaagccatgctaaacaatgctcaa
ccagatcaatatgaagctccagataaagattttatgatagtggctcttga
tttactgagtggcctggctgaaggacttggaggcaacattgaacagctgg
tagcccgaagtaacatcctgacactaatgtatcagtgcatgcaggataaa
atgccagaagttcgacagagttcttttgccctgttaggtgacctcacaaa
agcttgctttcagcatgttaagccttgtatagctgatttcatgccaatat
tgggaaccaacctaaatccagaattcatttcagtctgcaacaatgccaca
tgggcaattggagaaatctccattcaaatgggGatTgaAatgcaAccGta
tattcctatggtgttgcaccagcttgtagaaatcattaacagacccaaca
caccaaagacgttgttagagaatacagcaataacaattggtcgtcttggt
tacgtttgtcctcaagaggtggcccccatgctacagcagtttataagacc
ctggtgcacctctctgagaaacataagagacaatgaggaaaaggattcag
cattccgtggaatttgtaccatgatcagtgtgaatcccagtggcgtaatc
caagattttatatttttttgtgatgccgttgcatcatggattaacccaaa
agatgatctcagagacatgttctgtaagatccttcatggatttaaaaatc
aagttggcgatgaaaattggaggcgtttctctgaccagtttcctcttccc
ttaaaagagcgtcttgcagctttttatggtgtttaagcggccgc

Amino Acid Sequence

MEQKLISEEDLMVWDRQTKMEYEWKPDEQGLQQILQLLKESQSPDTTIQR
TVQQKLEQLNQYPDFNNYLIFVLTKLKSEDEPTRSLSGLILKNNVKAHFQ
NFPNGVTDFIKSECLNNIGDSSPLIRATVGILITTIASKGELQNWPDLLP
KLCSLLDSEDYNTCEGAFGALQKICEDSAEILDSDVLDRPLNIMIPKFLQ
FFKHSSPKIRSHAVACVNQFIISRTQALMLHIDSFIENLFALAGDEEPEV
RKNVCRALVMLLEVRMDRLLPHMHNIVEYMLQRTQDQDENVALEACEFWL
TLAEQPICKDVLVRHLPKLIPVLVNGMKYSDIDIILLKGDVEEDETIPDS
EQDIRPRFHRSRTVAQQHDEDGIEEEDDDDDEIDDDDTISDWNLRKCSAA
ALDVLANVYRDELLPHILPLLKELLFHHEWVVKESGILVLGAIAEGCMQG
MIPYLPELIPHLIQCLSDKKALVRSITCWTLSRYAHWVVSQPPDTYLKPL
MTELLKRILDSNKRVQEAACSAFATLEEEACTELVPYLAYILDTLVFAFS
KYQHKNLLILYDAIGTLADSVGHHLNKPEYIQMLMPPLIQKWNMLKDEDK
DLFPLLECLSSVATALQSGFLPYCEPVYQRCVNLVQKTLAQAMLNNAQPD
QYEAPDKDFMIVALDLLSGLAEGLGGNIEQLVARSNILTLMYQCMQDKMP
EVRQSSFALLGDLTKACFQHVKPCIADFMPILGTNLNPEFISVCNNATWA
IGEISIQMGIEMQPYIPMVLHQLVEIINRPNTPKTLLENTAITIGRLGYV
CPQEVAPMLQQFIRPWCTSLRNIRDNEEKDSAFRGICTMISVNPSGVIQD
FIFFCDAVASWINPKDDLRDMFCKILHGFKNQVGDENWRRFSDQFPLPLK
ERLAAFYGV*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pcDNA5 FRT/TO Myc-TNPOI WBL131.16 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU42506
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Can you please prepare TNPO1 resistant to siRNA (details send by email)
• Low Temp: No