cDNA Clone MLH1

• Synonyms: COCA2, FCC2, hMLH1, HNPCC, HNPCC2
• Plasmid: pcDNA5 FRT/TO GFP MLH1
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP-MLH1
• Genbank: NM_000249.3 GI:263191547 with change g1075a (V359M) - origially EST-derived and present in many published sequences for MLH1
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGTCGTTCGTGGCAGGGGTTATTCGGCGGCTGGACGAGACAGT
GGTGAACCGCATCGCGGCGGGGGAAGTTATCCAGCGGCCAGCTAATGCTA
TCAAAGAGATGATTGAGAACTGTTTAGATGCAAAATCCACAAGTATTCAA
GTGATTGTTAAAGAGGGAGGCCTGAAGTTGATTCAGATCCAAGACAATGG
CACCGGGATCAGGAAAGAAGATCTGGATATTGTATGTGAAAGGTTCACTA
CTAGTAAACTGCAGTCCTTTGAGGATTTAGCCAGTATTTCTACCTATGGC
TTTCGAGGTGAGGCTTTGGCCAGCATAAGCCATGTGGCTCATGTTACTAT
TACAACGAAAACAGCTGATGGAAAGTGTGCATACAGAGCAAGTTACTCAG
ATGGAAAACTGAAAGCCCCTCCTAAACCATGTGCTGGCAATCAAGGGACC
CAGATCACGGTGGAGGACCTTTTTTACAACATAGCCACGAGGAGAAAAGC
TTTAAAAAATCCAAGTGAAGAATATGGGAAAATTTTGGAAGTTGTTGGCA
GGTATTCAGTACACAATGCAGGCATTAGTTTCTCAGTTAAAAAACAAGGA
GAGACAGTAGCTGATGTTAGGACACTACCCAATGCCTCAACCGTGGACAA
TATTCGCTCCATCTTTGGAAATGCTGTTAGTCGAGAACTGATAGAAATTG
GATGTGAGGATAAAACCCTAGCCTTCAAAATGAATGGTTACATATCCAAT
GCAAACTACTCAGTGAAGAAGTGCATCTTCTTACTCTTCATCAACCATCG
TCTGGTAGAATCAACTTCCTTGAGAAAAGCCATAGAAACAGTGTATGCAG
CCTATTTGCCCAAAAACACACACCCATTCCTGTACCTCAGTTTAGAAATC
AGTCCCCAGAATGTGGATGTTAATGTGCACCCCACAAAGCATGAAGTTCA
CTTCCTGCACGAGGAGAGCATCCTGGAGCGGGTGCAGCAGCACATCGAGA
GCAAGCTCCTGGGCTCCAATTCCTCCAGGATGTACTTCACCCAGACTTTG
CTACCAGGACTTGCTGGCCCCTCTGGGGAGaTGGTTAAATCCACAACAAG
TCTGACCTCGTCTTCTACTTCTGGAAGTAGTGATAAGGTCTATGCCCACC
AGATGGTTCGTACAGATTCCCGGGAACAGAAGCTTGATGCATTTCTGCAG
CCTCTGAGCAAACCCCTGTCCAGTCAGCCCCAGGCCATTGTCACAGAGGA
TAAGACAGATATTTCTAGTGGCAGGGCTAGGCAGCAAGATGAGGAGATGC
TTGAACTCCCAGCCCCTGCTGAAGTGGCTGCCAAAAATCAGAGCTTGGAG
GGGGATACAACAAAGGGGACTTCAGAAATGTCAGAGAAGAGAGGACCTAC
TTCCAGCAACCCCAGAAAGAGACATCGGGAAGATTCTGATGTGGAAATGG
TGGAAGATGATTCCCGAAAGGAAATGACTGCAGCTTGTACCCCCCGGAGA
AGGATCATTAACCTCACTAGTGTTTTGAGTCTCCAGGAAGAAATTAATGA
GCAGGGACATGAGGTTCTCCGGGAGATGTTGCATAACCACTCCTTCGTGG
GCTGTGTGAATCCTCAGTGGGCCTTGGCACAGCATCAAACCAAGTTATAC
CTTCTCAACACCACCAAGCTTAGTGAAGAACTGTTCTACCAGATACTCAT
TTATGATTTTGCCAATTTTGGTGTTCTCAGGTTATCGGAGCCAGCACCGC
TCTTTGACCTTGCCATGCTTGCCTTAGATAGTCCAGAGAGTGGCTGGACA
GAGGAAGATGGTCCCAAAGAAGGACTTGCTGAATACATTGTTGAGTTTCT
GAAGAAGAAGGCTGAGATGCTTGCAGACTATTTCTCTTTGGAAATTGATG
AGGAAGGGAACCTGATTGGATTACCCCTTCTGATTGACAACTATGTGCCC
CCTTTGGAGGGACTGCCTATCTTCATTCTTCGACTAGCCACTGAGGTGAA
TTGGGACGAAGAAAAGGAATGTTTTGAAAGCCTCAGTAAAGAATGCGCTA
TGTTCTATTCCATCCGGAAGCAGTACATATCTGAGGAGTCGACCCTCTCA
GGCCAGCAGAGTGAAGTGCCTGGCTCCATTCCAAACTCCTGGAAGTGGAC
TGTGGAACACATTGTCTATAAAGCCTTGCGCTCACACATTCTGCCTCCTA
AACATTTCACAGAAGATGGAAATATCCTGCAGCTTGCTAACCTGCCTGAT
CTATACAAAGTCTTTGAGAGGTGTTAAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMSFVAG
VIRRLDETVVNRIAAGEVIQRPANAIKEMIENCLDAKSTSIQVIVKEGGL
KLIQIQDNGTGIRKEDLDIVCERFTTSKLQSFEDLASISTYGFRGEALAS
ISHVAHVTITTKTADGKCAYRASYSDGKLKAPPKPCAGNQGTQITVEDLF
YNIATRRKALKNPSEEYGKILEVVGRYSVHNAGISFSVKKQGETVADVRT
LPNASTVDNIRSIFGNAVSRELIEIGCEDKTLAFKMNGYISNANYSVKKC
IFLLFINHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISPQNVDVN
VHPTKHEVHFLHEESILERVQQHIESKLLGSNSSRMYFTQTLLPGLAGPS
GEMVKSTTSLTSSSTSGSSDKVYAHQMVRTDSREQKLDAFLQPLSKPLSS
QPQAIVTEDKTDISSGRARQQDEEMLELPAPAEVAAKNQSLEGDTTKGTS
EMSEKRGPTSSNPRKRHREDSDVEMVEDDSRKEMTAACTPRRRIINLTSV
LSLQEEINEQGHEVLREMLHNHSFVGCVNPQWALAQHQTKLYLLNTTKLS
EELFYQILIYDFANFGVLRLSEPAPLFDLAMLALDSPESGWTEEDGPKEG
LAEYIVEFLKKKAEMLADYFSLEIDEEGNLIGLPLLIDNYVPPLEGLPIF
ILRLATEVNWDEEKECFESLSKECAMFYSIRKQYISEESTLSGQQSEVPG
SIPNSWKWTVEHIVYKALRSHILPPKHFTEDGNILQLANLPDLYKVFERC
*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU45229
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee