cDNA Clone TNPO1

• Plasmid: pET15b 6His -TNPOI
• Parent Plasmid: pET15b 6His 3C
• Expressed: 6His-TNPOI
• Genbank: NCBI ID: 3842
• Tag: 6His
• Species: Human

Sequence of Insert

atggtgtgggaccggcaaaccaagatggagtatgagtggaaacctgacga
gcaagggcttcagcaaatcctgcagctgttgaaggagtcccagtccccag
acaccaccatccagagaaccgtgcaacaaaaactggaacaacttaatcag
tatccagactttaacaactacttgatttttgttcttacaaaattaaaatc
tgaagatgaacccacaagatcattgagtggtcttatcttgaagaataatg
tgaaagcacactttcagaacttcccaaatggtgtaacagactttattaaa
agtgaatgtttaaataatattggtgactcctctcctctgattagagccac
tgttggtattttgatcacaactatagcctccaagggagaattgcagaatt
ggcctgacctcttaccaaaactctgtagcctgttggattctgaagattat
aatacctgtgagggagcatttggtgcccttcagaagatttgtgaagattc
tgctgagattttagacagtgatgttttagatcgtcctctcaacatcatga
ttcccaaatttttacagttcttcaagcatagtagtccaaaaataaggtct
cacgctgttgcatgtgtcaatcagtttatcatcagtaggactcaagctct
aatgttgcacattgattcttttattgagaatctctttgcattagctggtg
atgaagaaccagaggtacggaaaaatgtgtgccgagcacttgtgatgttg
ctcgaagttcgaatggatcgcctgcttcctcacatgcataatatagttga
gtacatgctacagaggactcaagatcaagatgaaaatgtggctttagaag
cctgtgaattttggctaactttagctgaacagccaatatgcaaagatgta
ctcgtaaggcatcttcctaagttgattcctgtgttagtgaatggcatgaa
gtactcagacatagatattatcctacttaagggtgatgttgaagaagacg
aaacgattcctgatagtgaacaggatatacggccacgttttcaccgatcg
aggacggtggctcagcagcatgatgaagatggaattgaagaggaagatga
tgatgatgatgaaattgatgatgatgatacaatttctgactggaatctaa
gaaaatgttctgctgctgccctggatgttcttgcaaatgtgtatcgtgat
gaactgctgccacatattttgccccttttgaaagaattactttttcatca
tgaatgggttgttaaagaatcaggcattttggttttaggagcaattgctg
aaggttgcatgcagggcatgattccatacttgcctgagcttattcctcac
cttattcagtgcctctctgataaaaaggctcttgtgcgttccataacatg
ctggactcttagccgctatgcacactgggtggtcagccagccgccagaca
cgtacctgaagccattaatgacagaattgctaaagcgcatcctggacagc
aacaagagagtacaagaagctgcctgcagtgcctttgctaccctagaaga
ggaggcttgtacagaacttgttccttaccttgcttatatacttgataccc
tggtctttgcatttagtaaataccagcataagaacctgctcattctttac
gatgccataggaacattagcagattcagtaggacatcatttaaacaaacc
agaatatattcagatgctaatgcctccactgatccagaaatggaacatgt
taaaggatgaagataaagatctcttccctttacttgagtgcctatcttca
gttgccacagcactgcagtctggattccttccgtactgtgaacctgtgta
tcagcgttgtgtaaacctagtacagaagactcttgcacaagccatgctaa
acaatgctcaaccagatcaatatgaagctccagataaagattttatgata
gtggctcttgatttactgagtggcctggctgaaggacttggaggcaacat
tgaacagctggtagcccgaagtaacatcctgacactaatgtatcagtgca
tgcaggataaaatgccagaagttcgacagagttcttttgccctgttaggt
gacctcacaaaagcttgctttcagcatgttaagccttgtatagctgattt
catgccaatattgggaaccaacctaaatccagaattcatttcagtctgca
acaatgccacatgggcaattggagaaatctccattcaaatgggtatagag
atgcagccttatattcctatggtgttgcaccagcttgtagaaatcattaa
cagacccaacacaccaaagacgttgttagagaatacagcaataacaattg
gtcgtcttggttacgtttgtcctcaagaggtggcccccatgctacagcag
tttataagaccctggtgcacctctctgagaaacataagagacaatgagga
aaaggattcagcattccgtggaatttgtaccatgatcagtgtgaatccca
gtggcgtaatccaagattttatatttttttgtgatgccgttgcatcatgg
attaacccaaaagatgatctcagagacatgttctgtaagatccttcatgg
atttaaaaatcaagttggcgatgaaaattggaggcgtttctctgaccagt
ttcctcttcccttaaaagagcgtcttgcagctttttatggtgtttaa

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSMVWDRQTKMEYEWKPDEQGLQQILQLLKESQ
SPDTTIQRTVQQKLEQLNQYPDFNNYLIFVLTKLKSEDEPTRSLSGLILK
NNVKAHFQNFPNGVTDFIKSECLNNIGDSSPLIRATVGILITTIASKGEL
QNWPDLLPKLCSLLDSEDYNTCEGAFGALQKICEDSAEILDSDVLDRPLN
IMIPKFLQFFKHSSPKIRSHAVACVNQFIISRTQALMLHIDSFIENLFAL
AGDEEPEVRKNVCRALVMLLEVRMDRLLPHMHNIVEYMLQRTQDQDENVA
LEACEFWLTLAEQPICKDVLVRHLPKLIPVLVNGMKYSDIDIILLKGDVE
EDETIPDSEQDIRPRFHRSRTVAQQHDEDGIEEEDDDDDEIDDDDTISDW
NLRKCSAAALDVLANVYRDELLPHILPLLKELLFHHEWVVKESGILVLGA
IAEGCMQGMIPYLPELIPHLIQCLSDKKALVRSITCWTLSRYAHWVVSQP
PDTYLKPLMTELLKRILDSNKRVQEAACSAFATLEEEACTELVPYLAYIL
DTLVFAFSKYQHKNLLILYDAIGTLADSVGHHLNKPEYIQMLMPPLIQKW
NMLKDEDKDLFPLLECLSSVATALQSGFLPYCEPVYQRCVNLVQKTLAQA
MLNNAQPDQYEAPDKDFMIVALDLLSGLAEGLGGNIEQLVARSNILTLMY
QCMQDKMPEVRQSSFALLGDLTKACFQHVKPCIADFMPILGTNLNPEFIS
VCNNATWAIGEISIQMGIEMQPYIPMVLHQLVEIINRPNTPKTLLENTAI
TIGRLGYVCPQEVAPMLQQFIRPWCTSLRNIRDNEEKDSAFRGICTMISV
NPSGVIQDFIFFCDAVASWINPKDDLRDMFCKILHGFKNQVGDENWRRFS
DQFPLPLKERLAAFYGV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU42534
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Can you please clone TNPO1 into a bacterial expression vector (Nifty or equivalent) with N-term 6xHis tag