cDNA Clone ZNRF4

• Plasmid: pLou3-ZNRF4
• Parent Plasmid: pLOU3
• Expressed: HIS-MBP-ZNRF4
• Genbank: 148066
• Tag: 6His
• Tag: MBP
• Species: Human

Sequence of Insert

GGATCCATGCCGCTCTGCCGTCCGGAGCACTTAATGCCTAGAGCCAGCAGGGTCCCAGTGGCCGCGTCACTGCCTCTGAGCCACGCGGTCATTCCAACTCAACTGCCCTCGCGTCCTGGCCACAGGCCCCCTGGGAGACCCCGGAGATGCCCAAAGGCCTCATGCCTGCCGCCTCCAGTGGGACCTAGCAGCACACAGACAGCGAAGCGGGTGACCATGGGGTGGCCACGGCCGGGCCGAGCCCTCGTGGCAGTCAAAGCCTTGCTGGTCTTGTCGCTGCTCCAGGTGCCCGCGCAGGCAGTGGTACGGGCCGTGCTGGAAGACAACTCGAGCTCGGTGGACTTTGCGGATCTGCCGGCGCTGTTCGGCGTCCCCCTGGCCCCCGAGGGCATACGGGGCTACCTGATGGAGGTCAAGCCAGCCAACGCGTGCCATCCCATCGAGGCCCCGCGACTGGGCAACCGCTCTCTGGGCGCCATCGTGCTGATCCGCCGCTACGACTGCACCTTCGACCTCAAGGTGCTGAACGCCCAGCGCGCCGGCTTCGAGGCGGCCATCGTGCACAACGTCCACTCCGACGACCTCGTGAGCATGACCCACGTCTACGAGGACTTGAGGGGCCAGATCGCCATCCCCTCAGTGTTCGTGAGCGAGGCCGCCTCGCAGGACCTGCGGGTCATCCTGGGCTGCAACAAGTCGGCCCACGCGCTGCTCCTGCCCGACGACCCACCGTGCCACGACCTGGGCTGTCACCCCGTGCTGACCGTGTCCTGGGTGCTGGGCTGTACCCTGGCCCTGGTCGTATCAGCCTTCTTTGTCCTGAACCACCTGTGGCTCTGGGCCCAGGCCTGCTGCAGCCACAGACGGCCGGTGAAGACGTCTACCTGCCAGAAGGCCCAGGTCCGCACCTTCACGTGGCACAACGACCTGTGTGCCATCTGCCTGGATGAGTATGAGGAGGGCGACCAACTCAAGATCCTGCCCTGCTCCCACACCTACCACTGCAAATGCATTGACCCCTGGTTCTCCCAAGCCCCCCGGCGCTCCTGCCCCGTGTGCAAACAGTCGGTGGCCGCCACAGAAGACAGCTTTGACTCCACCACCTACAGCTTCAGGGACGAGGACCCCTCCCTACCGGGCCACCGGCCCCCCATCTGGGCCATTCAAGTCCAGCTACGCTCCCGGAGGCTGGAGCTGCTGGGCCGCGCCAGTCCCCACTGCCACTGCAGCACCACGTCCCTGGAGGCAGAGTATACCACTGTCTCCTCAGCCCCTCCTGAGGCCCCTGGTCAGTAAGCGGCCGC

Amino Acid Sequence

MHHHHHHMKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSSNNNNNNNNNNLGENLYFQGAMDHVEFGSMPLCRPEHLMPRASRVPVAASLPLSHAVIPTQLPSRPGHRPPGRPRRCPKASCLPPPVGPSSTQTAKRVTMGWPRPGRALVAVKALLVLSLLQVPAQAVVRAVLEDNSSSVDFADLPALFGVPLAPEGIRGYLMEVKPANACHPIEAPRLGNRSLGAIVLIRRYDCTFDLKVLNAQRAGFEAAIVHNVHSDDLVSMTHVYEDLRGQIAIPSVFVSEAASQDLRVILGCNKSAHALLLPDDPPCHDLGCHPVLTVSWVLGCTLALVVSAFFVLNHLWLWAQACCSHRRPVKTSTCQKAQVRTFTWHNDLCAICLDEYEEGDQLKILPCSHTYHCKCIDPWFSQAPRRSCPVCKQSVAATEDSFDSTTYSFRDEDPSLPGHRPPIWAIQVQLRSRRLELLGRASPHCHCSTTSLEAEYTTVSSAPPEAPGQ*AA

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU23808
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: silent mutations at nt a294g t948g