cDNA Clone gamma toxin (K. lactis)

• Plasmid: pRS416-GalL-gamma toxin
• Parent Plasmid: pRS416-GalL
• Expressed: gamma toxin (K. lactis)

Sequence of Insert

GGATCCatggcagctactactgcgagagaggagtttttcttatgttatgatttaattagatatttaaaacaatatgaaaaaacaggagagagtaaattagtagaacaaacattttttaatagtattaaaaacttagacataaactctagagagtatatggaacttgtatataacaaaatagcaggtatttccaatgaaagaaataaatttgaaaatatatataaagatggagattctataagtcaagttgtagaaagagctgtaagcgaaaagaaacttacatttggattaaacggtaaaggattatatgttccagaaaacggagaaccccgactaaaaggttatgcttctattatagaaagaataactctggatttaatggaaatatattctattaaaggacttaatgatatacctagagatataaaatttaatatggaaaaaataagacaagaaagatacaaccaaatgaaagaagctctaaatagtgttgaaggttataaaggaaaaattgtagcctcagactcagattggtgtttcaaagatcctcaaggcaatagaataacagattttgatagtattaataaagaattaggtcttggtagaagagatgtaaaattagataaaggtcatgatgatttaattaaattatgtactgaaaaaatagatagtatgaataatctacagaatggaaaatgtgtataataaGAATTC

Amino Acid Sequence

MAATTAREEFFLCYDLIRYLKQYEKTGESKLVEQTFFNSIKNLDINSREYMELVYNKIAGISNERNKFENIYKDGDSISQVVERAVSEKKLTFGLNGKGLYVPENGEPRLKGYASIIERITLDLMEIYSIKGLNDIPRDIKFNMEKIRQERYNQMKEALNSVEGYKGKIVASDSDWCFKDPQGNRITDFDSINKELGLGRRDVKLDKGHDDLIKLCTEKIDSMNNLQNGKCV**

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU23786
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: flanked by GalL Pr and cyc terminator