cDNA Clone NLGN3 (mouse)

• Synonyms: Mouse Neuroligin 3
• Plasmid: pSC-B Nlgn3 with HA inserted
• Parent Plasmid: pSC-B
• Expressed: Signal Peptide-HA-NLGN3
• Genbank: NM_172932.4
• Tag: HA
• Species: Mouse

Sequence of Insert

GAATTCatgtggctgcagccctcgctgtccctgagccccacgcccacagttggccggagcctgtgcctcaccctgggcttcctcagtttggtgctgagggccagtacctacccatacgatgtgccagattacgcccaggccccggcacccacagtcaatactcactttgggaagctaaggggtgccagagtaccattgcccagtgaaatcctgggtcctgtggaccaatacctgggggtaccctacgcagctcccccgatcggcgagaaacgtttcctgccccctgaaccacccccatcctggtcgggcatccggaacgccacacactttcccccagtgtgcccccagaacatccacacagctgtgcccgaagtcatgctgccagtctggttcactgccaacttggatatcgtcgccacttatatccaggagcccaacgaagattgcctctatctgaatgtgtatgtgcccacggaagatggatccggcgctaagaaacagggcgaggacttagcggataatgacggggatgaagatgaagacatccgagacagtggtgctaaacctgtcatggtctacatccacggaggctcttacatggaaggaacaggcaacatgattgatggcagtgttcttgcaagttacggcaacgtcatcgtcatcaccctcaactatcgggtcggggtgctaggtttcctgagcactggagatcaggctgccaagggcaactatgggctccttgatcaaatccaggcccttcgctgggtgagtgagaatattgccttctttggaggagatccccgtagaattactgtctttggctctggcatcggtgcatcctgtgtcagtctccttacactgtctcatcattctgaggggcttttccagagggccatcatccaaagtggctctgctctatctagctgggctgtgaactaccaaccagtgaagtataccagcttgctggcagacaaagtgggctgtaacgtcctggacactgtggatatggtggattgtcttcgacaaaagagtgccaaggagctggtagaacaggacattcagccagcccgctaccatgtggcttttggccctgtgattgatggtgatgtcattcctgatgaccctgagatccttatggagcagggagagttcctcaactatgatatcatgctaggcgtcaaccagggtgagggtctcaagtttgtggaaggggtggtggaccccgaggatggtgtctcgggcactgactttgactactctgtctccaattttgtggacaatctgtatggctatcctgagggtaaggacaccctgcgggagactatcaagttcatgtatacggactgggcagaccgagacaaccctgagacccgccgtaaaacactggtggcactcttcactgaccaccagtgggtggagccttcagtggtgacagccgatctgcacgcccgctatggctcacctacctacttctacgccttctaccatcactgccagagcctcatgaagcccgcatggtcagatgcagcacacggggatgaagtgccctatgtttttggtgtccctatggtaggtcccactgaccttttcccctgcaacttctccaagaatgatgttatgctcagtgctgtcgtcatgacctattggaccaactttgccaagaccggggatcccaacaagccggtaccccaggataccaagttcattcacaccaaggccaaccgctttgaggaagtggcctggtccaaatacaatccccgagaccagctctaccttcacatcgggctgaaaccaagggttcgtgatcattaccgggccacaaaggtagccttttggaaacacctggtgccccacctgtacaacctgcatgacatgttccactatacatccacgaccaccaaagtgccgcccccggacaccacccacagctcccacatcacccgtaggcccaacggcaagacctggagcaccaagcggccggcgatttcacctgcctacagcaatgagaatgcccctgggtcctggaatggggaccaggatgcggggccactcctggttgagaaccctcgagactactccactgaattaagtgtcactatcgctgtgggggcctccctcctgtttctcaatgtgttggcctttgctgccctctattaccgtaaggacaaacggcgccaggagcccctgaggcagcctagcccccaaaggggaactggtgcccctgaattgggaactgctccggaggaggagctggcagcattacagttgggtcccactcaccatgaatgtgaggccggtcccccacatgacacacttcgcctcacagcactgcccgactataccctgaccctgcggcgctcccctgatgacatcccactcatgactcccaacaccatcactatgattcctaattccctggttgggttgcagaccttgcacccctataacacctttgccgcagggttcaacagtactgggctgccccactcacactccactacccgtgtatagGCGGCCGC

Amino Acid Sequence

MWLQPSLSLSPTPTVGRSLCLTLGFLSLVLRASTYPYDVPDYAQAPAPTVNTHFGKLRGARVPLPSEILGPVDQYLGVPYAAPPIGEKRFLPPEPPPSWSGIRNATHFPPVCPQNIHTAVPEVMLPVWFTANLDIVATYIQEPNEDCLYLNVYVPTEDGSGAKKQGEDLADNDGDEDEDIRDSGAKPVMVYIHGGSYMEGTGNMIDGSVLASYGNVIVITLNYRVGVLGFLSTGDQAAKGNYGLLDQIQALRWVSENIAFFGGDPRRITVFGSGIGASCVSLLTLSHHSEGLFQRAIIQSGSALSSWAVNYQPVKYTSLLADKVGCNVLDTVDMVDCLRQKSAKELVEQDIQPARYHVAFGPVIDGDVIPDDPEILMEQGEFLNYDIMLGVNQGEGLKFVEGVVDPEDGVSGTDFDYSVSNFVDNLYGYPEGKDTLRETIKFMYTDWADRDNPETRRKTLVALFTDHQWVEPSVVTADLHARYGSPTYFYAFYHHCQSLMKPAWSDAAHGDEVPYVFGVPMVGPTDLFPCNFSKNDVMLSAVVMTYWTNFAKTGDPNKPVPQDTKFIHTKANRFEEVAWSKYNPRDQLYLHIGLKPRVRDHYRATKVAFWKHLVPHLYNLHDMFHYTSTTTKVPPPDTTHSSHITRRPNGKTWSTKRPAISPAYSNENAPGSWNGDQDAGPLLVENPRDYSTELSVTIAVGASLLFLNVLAFAALYYRKDKRRQEPLRQPSPQRGTGAPELGTAPEEELAALQLGPTHHECEAGPPHDTLRLTALPDYTLTLRRSPDDIPLMTPNTITMIPNSLVGLQTLHPYNTFAAGFNSTGLPHSHSTTRV*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU22633
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Residues 1-34 corresponds to a Signal Peptide (SP) (1-34: MWLRLGPPSLSLSPKPTVGRSLCLTLWFLSLALRAST). The HA tag is cloned immediately after peptide 34. The rest of neuroligin 3 (38-end) is cloned after the tag. Has a non silent mutation that needs corrected c1652t