cDNA Clone SPAK

• Plasmid: pGEX6P SPAK/Enhancer WNK1 1-661
• Parent Plasmid: pGEX6P1
• Expressed: GST-SPAK
• Genbank: AAC72238.1 GI:3851171
• Tag: GST
• Species: Human

Sequence of Insert

ggatccgccATGGCGGAGCCGAGCGGCTCGCCCGTGCACGTCCAGCTTCCCCAGCAGGCGGCCCCGGTGACAGCGGCGGCGGCGGCGGCCCCGGCGGCCGCGACAGCAGCGCCGGCCCCGGCAGCTCCCGCGGCCCCGGCCCCGGCCCCGGCCCCGGCCCCGGCGGCACAGGCTGTCGGCTGGCCCATCTGCAGGGACGCGTACGAGCTGCAGGAGGTTATCGGCAGTGGAGCTACTGCTGTGGTTCAGGCAGCCCTATGCAAACCCAGGCAAGAACGTGTAGCAATAAAACGGATCAACTTGGAAAAATGCCAGACCAGTATGGATGAACTATTAAAAGAAATTCAAGCCATGAGTCAGTGCAGCCATCCCAACGTAGTGACCTATTACACCTCTTTTGTGGTCAAAGATGAACTTTGGCTGGTCATGAAATTACTAAGTGGAGGTTCAATGTTGGATATCATAAAATACATTGTCAACCGAGGAGAACACAAGAATGGAGTTCTGGAAGAGGCAATAATAGCAACAATTCTTAAAGAGGTTTTGGAAGGCTTAGACTATCTACACAGAAACGGTCAGATTCACAGGGATTTGAAAGCTGGTAATATTCTTCTGGGTGAGGATGGTTCAGTACAAATAGCAGATTTTGGGGTAAGTGCGTTCCTAGCAACAGGGGGTGATGTTACCCGAAATAAAGTAAGAAAAACATTCGTTGGCACCCCATGTTGGATGGCTCCTGAAGTCATGGAACAGGTGAGAGGCTATGACTTCAAGGCTGACATGTGGAGTTTTGGAATAACTGCCATTGAATTAGCAACAGGAGCAGCGCCTTATCACAAATATCCTCCCATGAAAGTGTTAATGTTGACTTTGCAAAATGATCCACCCACTTTGGAAACAGGGGTAGAGGATAAAGAAATGATGAAAAAGTACGGCAAGTCCTTTAGAAAATTACTTTCACTGTGTCTTCAGAAAGATCCTTCCAAAAGGCCCACAGCAGCAGAACTTTTAAAATGCAAATTCTTCCAGAAAGCCAAGAACAGAGAGTACCTGATTGAGAAGCTGCTTACAAGAACACCAGACATAGCCCAAAGAGCCAAAAAGGTAAGAAGAGTTCCTGGGTCAAGTGGTCACCTTCATAAAACCGAAGACGGGGACTGGGAGTGGAGTGACGACGAGATGGATGAGAAGAGCGAAGAAGGGAAAGCAGCTTTTTCTCAGGAAAAGTCACGAAGAGTAAAAGAAGAAAATCCAGAGATTGCAGTGAGTGCCAGCACCATCCCCGAACAAATACAGTCCCTCTCTGTGCACGACTCTCAGGGCCCACCCAATGCTAATGAAGACTACAGAGAAGCTTCTTCTTGTGCCGTGAACCTCGTTTTGAGATTAAGAAACTCCAGAAAGGAACTTAATGACATACGATTTGAGTTTACTCCAGGAAGAGATACAGCAGATGGTGTATCTCAGGAGCTCTTCTCTGCTGGCTTGGTGGATGGTCACGATGTAGTTATAGTGGCTGCTAATTTACAGAAGATTGTAGATGATCCCAAAGCTTTAAAAACATTGACATTTAAGTTGGCTTCTGGCTGTGATGGGTCGGAGATTCCTGATGAAGTGAAGCTGATTGGGTTTGCTCAGTTGAGTGTCAGCTGAgtcgacgaagggcgaattc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAMAEPSGSPVHVQLPQQAAPVTAAAAAAPAAATAAPAPAAPAAPAPAPAPAPAAQAVGWPICRDAYELQEVIGSGATAVVQAALCKPRQERVAIKRINLEKCQTSMDELLKEIQAMSQCSHPNVVTYYTSFVVKDELWLVMKLLSGGSMLDIIKYIVNRGEHKNGVLEEAIIATILKEVLEGLDYLHRNGQIHRDLKAGNILLGEDGSVQIADFGVSAFLATGGDVTRNKVRKTFVGTPCWMAPEVMEQVRGYDFKADMWSFGITAIELATGAAPYHKYPPMKVLMLTLQNDPPTLETGVEDKEMMKKYGKSFRKLLSLCLQKDPSKRPTAAELLKCKFFQKAKNREYLIEKLLTRTPDIAQRAKKVRRVPGSSGHLHKTEDGDWEWSDDEMDEKSEEGKAAFSQEKSRRVKEENPEIAVSASTIPEQIQSLSVHDSQGPPNANEDYREASSCAVNLVLRLRNSRKELNDIRFEFTPGRDTADGVSQELFSAGLVDGHDVVIVAANLQKIVDDPKALKTLTFKLASGCDGSEIPDEVKLIGFAQLSVS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU40849
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Dual expression construct. WNK1 has pOPC enhancer sequence upstream.; ; All SPAK plasmids MUST be grown at 30 degrees C or less to prevent recombination.