cDNA Clone VCPIP1

• Synonyms: DUBA3, VCIP135
• Plasmid: pGEX6P-1 VCPIP1 92-463
• Parent Plasmid: pGEX6P1
• Expressed: GST N-term
• Genbank: NM_025054.4
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCCATGGACGTAGTGCTACACAACCTGCTGCGGAACGCGCTGCT
CGGGGTTACGGGGGCACCCAAGAAGAACACGGAACTGGTAAAGGTGATGG
GCCTTTCCAACTATCACTGCAAATTGTTGTCGCCCATATTAGCTCGCTAT
GGAATGGACAAACAGACAGGCCGGGCCAAGCTTCTCCGGGACATGAACCA
GGGCGAACTGTTCGATTGCGCCTTACTGGGTGACCGCGCCTTCCTCATAG
AACCAGAGCATGTTAACACTGTGGGCTATGGCAAGGACCGCTCCGGAAGC
CTCCTGTATTTGCATGACACTCTGGAGGACATTAAGCGGGCCAATAAAAG
CCAGGAATGTCTCATTCCAGTGCATGTGGACGGGGATGGACACTGCTTGG
TGCATGCTGTGTCTCGGGCTCTAGTAGGCCGAGAGCTCTTCTGGCATGCC
TTAAGAGAGAATCTTAAACAGCACTTTCAGCAGCACCTGGCCCGATATCA
AGCTCTGTTCCATGACTTCATTGATGCTGCTGAGTGGGAGGACATTATCA
ATGAGTGTGACCCTCTGTTTGTACCACCTGAGGGTGTTCCCTTGGGCCTG
AGGAATATCCACATATTTGGTCTTGCCAATGTGCTACATCGTCCTATTAT
TCTGTTAGATTCCCTCAGTGGCATGAGAAGCTCTGGTGATTATTCAGCCA
CCTTTCTACCTGGGCTCATCCCTGCAGAGAAGTGCACTGGGAAAGATGGT
CATTTGAACAAACCAATCTGTATTGCATGGAGCAGCTCCGGTAGAAACCA
TTATATCCCCTTGGTAGGCATAAAAGGGGCTGCTTTGCCCAAACTGCCTA
TGAATTTGCTTCCTAAAGCATGGGGTGTGCCTCAGGACCTTATTAAAAAG
TACATAAAACTTGAAGAGGATGGTGGTTGTGTTATTGGAGGTGACAGAAG
TTTGCAAGATAAATACTTACTTAGGCTTGTTGCTGCTATGGAAGAAGTCT
TTATGGACAAACATGGTATCCATCCTAGTTTGGTTGCTGATGTCCATCAG
TATTTCTACAGAAGGACTGGAGTGATAGGAGTTCAGCCTGAGGAAGTCAC
AGCAGCTGCTAAAAAAGCAGTAATGGATTAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAMDVVLHNLLRNALLGVTG
APKKNTELVKVMGLSNYHCKLLSPILARYGMDKQTGRAKLLRDMNQGELF
DCALLGDRAFLIEPEHVNTVGYGKDRSGSLLYLHDTLEDIKRANKSQECL
IPVHVDGDGHCLVHAVSRALVGRELFWHALRENLKQHFQQHLARYQALFH
DFIDAAEWEDIINECDPLFVPPEGVPLGLRNIHIFGLANVLHRPIILLDS
LSGMRSSGDYSATFLPGLIPAEKCTGKDGHLNKPICIAWSSSGRNHYIPL
VGIKGAALPKLPMNLLPKAWGVPQDLIKKYIKLEEDGGCVIGGDRSLQDK
YLLRLVAAMEEVFMDKHGIHPSLVADVHQYFYRRTGVIGVQPEEVTAAAK
KAVMD*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU44357
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: residues 92-463, precission cleavage site between GST and protein