cDNA Clone VCPIP1

• Synonyms: DUBA3, VCIP135
• Plasmid: pGEX6P-1 VCPIP1 92-856
• Parent Plasmid: pGEX6P1
• Expressed: GST N-term
• Genbank: NM_025054.4
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCGCCATGGACGTAGTGCTACACAACCTGCTGCGGAACGCGCTGCTCGGGGTTACGGGGGCACCCAAGAAGAACACGGAACTGGTAAAGGTGATGGGCCTTTCCAACTATCACTGCAAATTGTTGTCGCCCATATTAGCTCGCTATGGAATGGACAAACAGACAGGCCGGGCCAAGCTTCTCCGGGACATGAACCAGGGCGAACTGTTCGATTGCGCCTTACTGGGTGACCGCGCCTTCCTCATAGAACCAGAGCATGTTAACACTGTGGGCTATGGCAAGGACCGCTCCGGAAGCCTCCTGTATTTGCATGACACTCTGGAGGACATTAAGCGGGCCAATAAAAGCCAGGAATGTCTCATTCCAGTGCATGTGGACGGGGATGGACACTGCTTGGTGCATGCTGTGTCTCGGGCTCTAGTAGGCCGAGAGCTCTTCTGGCATGCCTTAAGAGAGAATCTTAAACAGCACTTTCAGCAGCACCTGGCCCGATATCAAGCTCTGTTCCATGACTTCATTGATGCTGCTGAGTGGGAGGACATTATCAATGAGTGTGACCCTCTGTTTGTACCACCTGAGGGTGTTCCCTTGGGCCTGAGGAATATCCACATATTTGGTCTTGCCAATGTGCTACATCGTCCTATTATTCTGTTAGATTCCCTCAGTGGCATGAGAAGCTCTGGTGATTATTCAGCCACCTTTCTACCTGGGCTCATCCCTGCAGAGAAGTGCACTGGGAAAGATGGTCATTTGAACAAACCAATCTGTATTGCATGGAGCAGCTCCGGTAGAAACCATTATATCCCCTTGGTAGGCATAAAAGGGGCTGCTTTGCCCAAACTGCCTATGAATTTGCTTCCTAAAGCATGGGGTGTGCCTCAGGACCTTATTAAAAAGTACATAAAACTTGAAGAGGATGGTGGTTGTGTTATTGGAGGTGACAGAAGTTTGCAAGATAAATACTTACTTAGGCTTGTTGCTGCTATGGAAGAAGTCTTTATGGACAAACATGGTATCCATCCTAGTTTGGTTGCTGATGTCCATCAGTATTTCTACAGAAGGACTGGAGTGATAGGAGTTCAGCCTGAGGAAGTCACAGCAGCTGCTAAAAAAGCAGTAATGGATAATCGCCTTCACAAATGTTTGCTCTGTGGTGCCCTTTCTGAACTTCATGTTCCTCCAGAGTGGTTGGCTCCTGGAGGGAAATTGTATAACCTGGCAAAAAGTACTCATGGACAGCTGAGGACTGACAAAAATTACAGCTTTCCCTTGAACAATTTGGTTTGCTCATATGATTCAGTGAAAGATGTTCTGGTACCAGACTATGGAATGAGTAACCTAACAGCTTGTAATTGGTGCCATGGCACATCTGTGCGAAAGGTCAGAGGAGATGGGTCTATTGTGTATTTGGATGGAGACAGAACTAATTCTAGGTCCACTGGTGGCAAATGTGGTTGTGGATTCAAACACTTTTGGGATGGTAAGGAGTATGACAATCTACCAGAAGCTTTCCCTATTACTTTAGAATGGGGTGGAAGAGTGGTCAGAGAAACAGTATATTGGTTCCAGTATGAAAGTGATTCATCTTTGAATAGTAATGTTTACGATGTTGCAATGAAACTTGTTACCAAGCACTTTCCAGGTGAATTTGGGAGTGAAATCCTAGTTCAGAAAGTTGTCCACACTATATTGCATCAGACTGCCAAAAAGAATCCCGATGATTATACTCCTGTAAATATAGATGGTGCTCACGCCCAAAGAGTTGGAGATGTTCAAGGACAAGAATCAGAGTCTCAGCTCCCAACTAAAATTATTCTTACTGGACAGAAAACAAAAACTTTGCACAAGGAGGAGTTAAACATGAGTAAAACTGAAAGAACTATTCAACAGAATATTACGGAACAGGCTTCTGTAATGCAGAAACGGAAAACAGAGAAGTTAAAACAAGAACAAAAAGGGCAACCCAGGACTGTTTCTCCCAGTACCATTCGTGATGGTCCATCCTCTGCACCTGCTACACCTACCAAGGCTCCCTATTCACCGACAACTTCTAAGGAGAAGAAGATCCGAATCACAACTAATGATGGACGACAGTCCATGGTTACCCTTAAGTCTTCAACAACCTTTTTTGAACTTCAGGAAAGTATAGCCAGAGAATTCAACATTCCTCCATATTTACAGTGTATTCGATACGGGTTTCCTCCTAAAGAGTTAATGCCACCACAGGCAGGAATGGAAAAGGAACCAGTTCCTTTACAGCATGGCGACAGAATTACAATAGAAATTCTAAAAAGTAAAGCTGAATAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAMDVVLHNLLRNALLGVTG
APKKNTELVKVMGLSNYHCKLLSPILARYGMDKQTGRAKLLRDMNQGELF
DCALLGDRAFLIEPEHVNTVGYGKDRSGSLLYLHDTLEDIKRANKSQECL
IPVHVDGDGHCLVHAVSRALVGRELFWHALRENLKQHFQQHLARYQALFH
DFIDAAEWEDIINECDPLFVPPEGVPLGLRNIHIFGLANVLHRPIILLDS
LSGMRSSGDYSATFLPGLIPAEKCTGKDGHLNKPICIAWSSSGRNHYIPL
VGIKGAALPKLPMNLLPKAWGVPQDLIKKYIKLEEDGGCVIGGDRSLQDK
YLLRLVAAMEEVFMDKHGIHPSLVADVHQYFYRRTGVIGVQPEEVTAAAK
KAVMDNRLHKCLLCGALSELHVPPEWLAPGGKLYNLAKSTHGQLRTDKNY
SFPLNNLVCSYDSVKDVLVPDYGMSNLTACNWCHGTSVRKVRGDGSIVYL
DGDRTNSRSTGGKCGCGFKHFWDGKEYDNLPEAFPITLEWGGRVVRETVY
WFQYESDSSLNSNVYDVAMKLVTKHFPGEFGSEILVQKVVHTILHQTAKK
NPDDYTPVNIDGAHAQRVGDVQGQESESQLPTKIILTGQKTKTLHKEELN
MSKTERTIQQNITEQASVMQKRKTEKLKQEQKGQPRTVSPSTIRDGPSSA
PATPTKAPYSPTTSKEKKIRITTNDGRQSMVTLKSSTTFFELQESIAREF
NIPPYLQCIRYGFPPKELMPPQAGMEKEPVPLQHGDRITIEILKSKAE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU44358
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: residues 92-856, precission cleavage site between GST and protein