cDNA Clone VPS16A

• Plasmid: pcDNA5 FRT/TO FLAG VPS16A
• Parent Plasmid: pcDNA5 FRT/TO FLAG
• Expressed: FLAG VPS16A
• Genbank: NM_022575.2 GI:17978478 with silent change to kill internal BglII
• Tag: FLAG
• Species: Human

Sequence of Insert

AGATCTATGGACTGCTACACGGCGAACTGGAACCCACTCGGGGACTCTGCCTTTTACCGGAAATATGAGCTGTACAGCATGGACTGGGACCTGAA
GGAGGAACTCAGGGATTGCCTGGTGGCTGCTGCACCCTATGGGGGCCCCATTGCACTGCTGAGGAACCCCTGGCGGAAGGAGAAAGCTGCTAGTGTGAGG
CCAGTGCTCGATATATACTCTGCTTCCGGCATGCCTCTGGCCAGCCTGCTGTGGAAGAGTGGACCCGTGGTGTCCCTGGGCTGGTCAGCTGAGGAGGAGC
TGCTCTGTGTGCAGGAAGATGGTGCTGTACTGGTTTATGGGCTTCATGGTGACTTCCGGAGACACTTCAGCATGGGCAATGAAGTGCTCCAGAACCGGGT
TCTGGATGCCCGGATCTTTCACACTGAGTTTGGTTCCGGAGTGGCCATCCTCACAGGGGCCCACCGCTTCACCCTCAGTGCCAATGTGGGTGACCTCAAA
CTCCGCCGGATGCCAGAGGTGCCAGGTCTGCAAAGTGCACCCTCCTGCTGGACTGTGCTGTGCCAGGACCGAGTGGCACACATTCTTCTGGCTGTGGGGC
CTGACCTTTACCTCTTGGACCATGCAGCCTGCTCCGCAGTGACGCCCCCTGGCCTGGCCCCAGGAGTAAGCAGCTTCCTACAGATGGCTGTCTCCTTCAC
CTACCGACACCTGGCACTCTTCACAGACACAGGCTACATCTGGATGGGGACAGCATCACTCAAGGAGAAGCTATGTGAGTTCAACTGCAACATCCGGGCA
CCTCCAAAGCAGATGGTCTGGTGCAGCCGTCCTCGTAGCAAGGAGAGGGCCGTGGTGGTGGCCTGGGAAAGGCGGCTGATGGTGGTGGGCGATGCACCCG
AGAGCATCCAGTTTGTGCTGGATGAGGACTCCTACCTGGTGCCTGAGCTCGATGGGGTCCGCATCTTCTCCCGCAGCACCCACGAGTTCCTGCATGAGGT
TCCAGCGGCCAGCGAGGAAATCTTCAAAATTGCCTCAATGGCCCCCGGGGCGCTGCTCCTGGAGGCTCAGAAGGAGTATGAGAAAGAGAGCCAGAAGGCG
GACGAGTACCTGCGGGAGATCCAGGAGCTGGGCCAGCTGACCCAGGCCGTGCAGCAGTGCATTGAGGCTGCAGGACATGAGCACCAGCCAGACATGCAGA
AGAGTCTGCTCAGGGCCGCCTCCTTCGGAAAGTGTTTCCTGGACAGATTTCCACCCGACAGCTTCGTGCACATGTGTCAGGACCTGCGTGTGCTCAATGC
TGTTCGGGACTATCACATCGGGATCCCGCTCACCTATAGCCAATATAAGCAGCTCACCATCCAGGTGCTGCTGGACAGGCTCGTGTTGCGGAGACTTTAC
CCCCTGGCCATCCAGATATGCGAGTACTTGCGCCTTCCTGAAGTACAGGGCGTCAGCAGGATCCTGGCCCACTGGGCCTGCTACAAGGTGCAACAGAAGG
ATGTCTCAGATGAGGATGTGGCTCGAGCCATTAACCAGAAGCTGGGGGACACGCCTGGTGTCTCTTACTCCGACATTGCTGCACGAGCCTATGGTTGTGG
CCGCACGGAGCTGGCCATCAAGCTGCTGGAGTATGAGCCACGCTCAGGGGAGCAGGTACCCCTTCTCCTAAAGATGAAGAGGAGCAAACTGGCACTAAGC
AAGGCCATCGAGAGCGGGGACACTGACCTGGTGTTCACGGTGTTGCTGCACCTGAAGAACGAGCTGAACCGAGGAGATTTTTTCATGACCCTTCGGAATC
AGCCCATGGCCCTCAGTTTGTACCGACAGTTCTGTAAGCATCAGGAGCTAGAGACGCTGAAGGACCTTTACAATCAGGATGACAATCACCAGGAATTGGG
CAGCTTCCACATCCGAGCCAGCTATGCTGCAGAAGAGCGTATTGAGGGGCGAGTAGCAGCTCTGCAGACAGCCGCCGATGCCTTCTACAAGGCCAAGAAT
GAGTTTGCAGCCAAGGCTACAGAGGATCAAATGCGGCTCCTACGGCTGCAGCGGCGCCTAGAAGACGAGCTGGGGGGCCAGTTCCTAGACCTGTCTCTAC
ATGACACAGTTACCACCCTCATTCTTGGCGGTCACAACAAGCGTGCAGAGCAGCTGGCACGTGACTTCCGCATCCCTGACAAGAGGCTCTGGTGGCTGAA
GCTGACTGCCCTGGCAGATTTGGAAGATTGGGAAGAGCTAGAGAAGTTTTCCAAGAGCAAGAAATCACCCATTGGCTACCTGCCTTTTGTGGAAATCTGC
ATGAAACAACATAACAAATACGAAGCCAAGAAGTATGCTTCCCGCGTGGGTCCCGAGCAGAAGGTCAAGGCTTTGCTTCTTGTTGGCGATGTGGCTCAGG
CTGCAGATGTGGCCATCGAACACCGGAATGAGGCTGAGCTGAGCCTCGTATTGTCCCACTGCACGGGAGCCACAGATGGGGCCACAGCTGACAAGATTCA
ACGGGCCAGGGCACAAGCCCAGAAGAAGTGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMDCYTANWNPLGDSAFYRKYELYSMDWDLKEELRDCLVA
AAPYGGPIALLRNPWRKEKAASVRPVLDIYSASGMPLASLLWKSGPVVSL
GWSAEEELLCVQEDGAVLVYGLHGDFRRHFSMGNEVLQNRVLDARIFHTE
FGSGVAILTGAHRFTLSANVGDLKLRRMPEVPGLQSAPSCWTVLCQDRVA
HILLAVGPDLYLLDHAACSAVTPPGLAPGVSSFLQMAVSFTYRHLALFTD
TGYIWMGTASLKEKLCEFNCNIRAPPKQMVWCSRPRSKERAVVVAWERRL
MVVGDAPESIQFVLDEDSYLVPELDGVRIFSRSTHEFLHEVPAASEEIFK
IASMAPGALLLEAQKEYEKESQKADEYLREIQELGQLTQAVQQCIEAAGH
EHQPDMQKSLLRAASFGKCFLDRFPPDSFVHMCQDLRVLNAVRDYHIGIP
LTYSQYKQLTIQVLLDRLVLRRLYPLAIQICEYLRLPEVQGVSRILAHWA
CYKVQQKDVSDEDVARAINQKLGDTPGVSYSDIAARAYGCGRTELAIKLL
EYEPRSGEQVPLLLKMKRSKLALSKAIESGDTDLVFTVLLHLKNELNRGD
FFMTLRNQPMALSLYRQFCKHQELETLKDLYNQDDNHQELGSFHIRASYA
AEERIEGRVAALQTAADAFYKAKNEFAAKATEDQMRLLRLQRRLEDELGG
QFLDLSLHDTVTTLILGGHNKRAEQLARDFRIPDKRLWWLKLTALADLED
WEELEKFSKSKKSPIGYLPFVEICMKQHNKYEAKKYASRVGPEQKVKALL
LVGDVAQAADVAIEHRNEAELSLVLSHCTGATDGATADKIQRARAQAQKK
*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU45352
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee