cDNA Clone ZNRF4

• Synonyms: spzn, RNF204, Ssrzf1, SPERIZIN
• Plasmid: pcDNA5D FRT/TO ZNRF4 C-FLAG
• Parent Plasmid: pcDNA5D FRT/TO C-FLAG
• Expressed: ZNRF4 C-FLAG
• Genbank: 148066
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCatgccgctctgccgtccggagcacttaatgcctagagccagcag
ggtcccagtggccgcgtcactgcctctgagccacgcggtcattccaactc
aactgccctcgcgtcctggccacaggccccctgggagaccccggagatgc
ccaaaggcctcatgcctgccgcctccagtgggacctagcagcacacagac
agcgaagcgggtgaccatggggtggccacggccgggccgagccctcgtgg
cagtcaaagccttgctggtcttgtcgctgctccaggtgcccgcgcaggcg
gtggtacgggccgtgctggaagacaactcgagctcggtggactttgcgga
tctgccggcgctgttcggcgtccccctggcccccgagggcatacggggct
acctgatggaggtcaagccagccaacgcgtgccatcccatcgaggccccg
cgactgggcaaccgctctctgggcgccatcgtgctgatccgccgctacga
ctgcaccttcgacctcaaggtgctgaacgcccagcgcgccggcttcgagg
cggccatcgtgcacaacgtccactccgacgacctcgtgagcatgacccac
gtctacgaggacttgaggggccagatcgccatcccctcagtgttcgtgag
cgaggccgcctcgcaggacctgcgggtcatcctgggctgcaacaagtcgg
cccacgcgctgctcctgcccgacgacccaccgtgccacgacctgggctgt
caccccgtgctgaccgtgtcctgggtgctgggctgtaccctggccctggt
cgtatcagccttctttgtcctgaaccacctgtggctctgggcccaggcct
gctgcagccacagacggccggtgaagacgtctacctgccagaaggcccag
gtccgcaccttcacgtggcacaacgacctgtgtgccatctgcctggatga
gtacgaggagggcgaccaactcaagatcctgccctgctcccacacctacc
actgcaaatgcattgacccctggttctcccaagccccccggcgctcctgc
cccgtgtgcaaacagtcggtggccgccacagaagacagctttgactccac
cacctacagcttcagggacgaggacccctccctaccgggccaccggcccc
ccatctgggccattcaagtccagctacgctcccggaggctggagctgctg
ggccgcgccagtccccactgccactgcagcaccacgtccctggaggcaga
gtataccactgtctcctcagcccctcctgaggcccctggtcagGCGGCCG
C

Amino Acid Sequence

MPLCRPEHLMPRASRVPVAASLPLSHAVIPTQLPSRPGHRPPGRPRRCPK
ASCLPPPVGPSSTQTAKRVTMGWPRPGRALVAVKALLVLSLLQVPAQAVV
RAVLEDNSSSVDFADLPALFGVPLAPEGIRGYLMEVKPANACHPIEAPRL
GNRSLGAIVLIRRYDCTFDLKVLNAQRAGFEAAIVHNVHSDDLVSMTHVY
EDLRGQIAIPSVFVSEAASQDLRVILGCNKSAHALLLPDDPPCHDLGCHP
VLTVSWVLGCTLALVVSAFFVLNHLWLWAQACCSHRRPVKTSTCQKAQVR
TFTWHNDLCAICLDEYEEGDQLKILPCSHTYHCKCIDPWFSQAPRRSCPV
CKQSVAATEDSFDSTTYSFRDEDPSLPGHRPPIWAIQVQLRSRRLELLGR
ASPHCHCSTTSLEAEYTTVSSAPPEAPGQAAADYKDDDDK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU23866
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 2 silent mutations at nt a294g t948g compared to NCBI ref