cDNA Clone USP15

• Plasmid: pcDNA frt to -FLAG-USP15-R C269S
• Parent Plasmid: pcDNA5 FRT/TO FLAG1
• Expressed: FLAG USP15-R C269S
• Genbank: NM_006313.1
• Tag: FLAG
• Species: Human

Sequence of Insert

ATGGCGGAAGGCGGAGCGGCGGATCTGGACACCCAGCGGTCTGACATCGCGACGCTGCTCAAAACCTCGCTCCGGAAAGGGGACACCTGGTACCTAGTCGATAGTCGCTGGTTCAAACAGTGGAAAAAATATGTTGGCTTTGACAGTTGGGACAAATACCAGATGGGAGATCAAAATGTGTATCCTGGACCCATTGATAACTCTGGACTTCTCAAAGATGGTGATGCCCAGTCACTTAAGGAACACCTTATTGATGAATTGGATTACATACTGTTGCCAACTGAAGGTTGGAATAAACTTGTCAGCTGGTACACgTTaATGGAgGGcCAAGAGCCAATAGCACGAAAGGTGGTTGAACAGGGTATGTTTGTAAAGCACTGCAAAGTAGAAGTATATCTCACAGAATTGAAGCTATGTGAAAATGGAAACATGAATAATGTTGTAACTCGAAGATTTAGCAAAGCTGACACAATAGATACAATTGAAAAGGAAATAAGAAAAATCTTCAGTATTCCAGATGAAAAGGAGACCAGATTGTGGAACAAATACATGAGTAACACATTTGAACCACTGAATAAACCAGACAGCACCATTCAGGATGCTGGTTTATACCAAGGACAGGTATTAGTGATAGAACAGAAAAATGAAGATGGAACATGGCCAAGGGGTCCTTCTACTCCTAATGTGAAAAACTCAAATTACTGTCTTCCATCATATACCGCTTATAAGAACTATGATTATTCGGAACCTGGAAGAAACAATGAACAGCCAGGCCTCTGTGGCCTAAGTAACTTGGGAAATACGTcaTTCATGAACTCAGCTATTCAGTGTTTGAGCAACACACCTCCACTTACTGAGTATTTCCTCAATGATAAGTATCAAGAAGAACTGAATTTTGACAATCCCTTAGGAATGAGAGGTGAAATAGCTAAATCTTATGCCGAACTGATCAAGCAAATGTGGTCTGGAAAGTTTAGCTACGTCACCCCAAGAGCCTTTAAGACACAGGTAGGACGTTTTGCACCTCAGTTCTCTGGATATCAGCAGCAAGACTGTCAAGAACTGTTAGCTTTCCTATTAGATGGATTACATGAGGATTTGAATAGAATTAGGAAAAAACCATATATACAATTAAAAGATGCAGATGGAAGGCCAGATAAGGTGGTTGCCGAAGAAGCCTGGGAAAACCATTTAAAACGAAATGATTCTATCATAGTAGATATATTTCATGGCCTTTTCAAATCAACTTTAGTTTGTCCTGAGTGTGCTAAGATTTCAGTAACATTTGATCCTTTTTGTTACTTGACACTTCCATTGCCCATGAAAAAAGAACGCACCTTGGAAGTTTACTTAGTTAGAATGGATCCGCTTACCAAACCTATGCAGTACAAAGTGGTTGTCCCCAAAATTGGAAACATATTAGATCTTTGTACAGCATTGTCTGCTTTGTCAGGAATACCTGCAGATAAGATGATAGTTACTGATATATACAATCATAGATTTCACAGAATATTCGCTATGGATGAAAACCTTAGTAGTATTATGGAACGGGATGATATTTATGTGTTTGAAATTAACATCAATAGGACAGAAGATACAGAGCACGTGATTATTCCTGTTTGCCTAAGAGAAAAATTCAGACACTCGAGTTATACCCACCATACTGGTTCTTCACTTTTTGGTCAGCCCTTTCTTATGGCTGTACCACGAAACAATACTGAAGACAAACTTTATAATCTCCTGCTCTTGAGAATGTGCCGATATGTCAAAATATCTACTGAAACTGAAGAAACTGAAGGATCCCTACACTGCTGTAAGGACCAAAATATTAATGGGAATGGCCCAAATGGCATACATGAAGAAGGCTCACCAAGTGAAATGGAAACAGATGAGCCAGATGATGAATCCAGCCAGGATCAAGAACTTCCCTCAGAGAATGAAAACAGTCAGTCTGAAGATTCAGTTGGAGGAGATAATGATTCTGAAAATGGATTATGTACTGAGGATACTTGCAAAGGTCAACTCACGGGACACAAAAAACGATTGTTTACATTCCAGTTCAACAACTTAGGCAATACTGATATCAACTACATCAAAGATGATACCAGGCATATAAGATTTGATGATAGGCAGCTTAGGCTAGATGAAAGATCTTTTCTTGCTTTGGATTGGGATCCTGATTTGAAAAAAAGATATTTTGATGAAAATGCTGCCGAGGACTTTGAAAAACATGAAAGTGTGGAGTATAAACCTCCTAAAAAACCCTTTGTGAAATTAAAAGATTGCATTGAACTTTTTACAACAAAAGAAAAGCTAGGTGCTGAAGATCCCTGGTATTGTCCGAATTGTAAAGAACATCAGCAAGCCACAAAGAAATTGGATTTATGGTCCCTGCCTCCAGTACTTGTAGTACATCTCAAGCGATTTTCTTACAGTCGATACATGAGAGACAAGTTGGATACCTTAGTTGATTTTCCTATCAATGACTTGGATATGTCGGAATTCTTAATTAATCCAAATGCAGGTCCTTGCCGCTATAATCTGATTGCTGTTTCCAACCACTATGGAGGGATGGGAGGAGGACACTATACTGCTTTTGCAAAAAATAAAGATGATGGAAAATGGTACTATTTTGATGACAGTAGTGTCTCCACTGCATCTGAAGACCAAATTGTGTCCAAAGCAGCATATGTACTCTTCTACCAGAGACAAGACACTTTCAGTGGAACTGGCTTTTTTCCTCTTGACCGAGAAACTAAAGGTGCTTCAGCTGCCACTGGCATCCCATTAGAAAGTGATGAAGATAGCAATGATAATGACAATGATATAGAAAATGAAAACTGTATGCACACTAACTAA

Amino Acid Sequence

MDYKDDDDKGSPEFPGRLERPLMAEGGAADLDTQRSDIATLLKTSLRKGD
TWYLVDSRWFKQWKKYVGFDSWDKYQMGDQNVYPGPIDNSGLLKDGDAQS
LKEHLIDELDYILLPTEGWNKLVSWYTLMEGQEPIARKVVEQGMFVKHCK
VEVYLTELKLCENGNMNNVVTRRFSKADTIDTIEKEIRKIFSIPDEKETR
LWNKYMSNTFEPLNKPDSTIQDAGLYQGQVLVIEQKNEDGTWPRGPSTPN
VKNSNYCLPSYTAYKNYDYSEPGRNNEQPGLCGLSNLGNTSFMNSAIQCL
SNTPPLTEYFLNDKYQEELNFDNPLGMRGEIAKSYAELIKQMWSGKFSYV
TPRAFKTQVGRFAPQFSGYQQQDCQELLAFLLDGLHEDLNRIRKKPYIQL
KDADGRPDKVVAEEAWENHLKRNDSIIVDIFHGLFKSTLVCPECAKISVT
FDPFCYLTLPLPMKKERTLEVYLVRMDPLTKPMQYKVVVPKIGNILDLCT
ALSALSGIPADKMIVTDIYNHRFHRIFAMDENLSSIMERDDIYVFEININ
RTEDTEHVIIPVCLREKFRHSSYTHHTGSSLFGQPFLMAVPRNNTEDKLY
NLLLLRMCRYVKISTETEETEGSLHCCKDQNINGNGPNGIHEEGSPSEME
TDEPDDESSQDQELPSENENSQSEDSVGGDNDSENGLCTEDTCKGQLTGH
KKRLFTFQFNNLGNTDINYIKDDTRHIRFDDRQLRLDERSFLALDWDPDL
KKRYFDENAAEDFEKHESVEYKPPKKPFVKLKDCIELFTTKEKLGAEDPW
YCPNCKEHQQATKKLDLWSLPPVLVVHLKRFSYSRYMRDKLDTLVDFPIN
DLDMSEFLINPNAGPCRYNLIAVSNHYGGMGGGHYTAFAKNKDDGKWYYF
DDSSVSTASEDQIVSKAAYVLFYQRQDTFSGTGFFPLDRETKGASAATGI
PLESDEDSNDNDNDIENENCMHTN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU42571
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: si Resistant USP15. Thank you Simone :)