cDNA Clone GFP

• Plasmid: pCRNT-TOPO-pLtetR-tetO-GFP kan out
• Parent Plasmid: pCRNT-TOPO-pLtetR-tetO
• Expressed: GFP kan out
• Tag: GFP

Sequence of Insert

ATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGA
ATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTG
AAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACT
GGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCTCTTATGG
TGTTCAATGCTTTTCCCGTTATCCGGATCACATGAAACGGCATGACTTTT
TCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAACGCACTATATCTTTC
AAAGATGACGGGAACTACAAGACGCGTGCTGAAGTCAAGTTTGAAGGTGA
TACCCTTGTTAATCGTATCGAGTTAAAAGGTATTGATTTTAAAGAAGATG
GAAACATTCTCGGACACAAACTCGAATACAACTATAACTCACACAATGTA
TACATCACGGCAGACAAACAAAAGAATGGAATCAAAGCTAACTTCAAAAT
TCGCCACAACATTGAAGATGGATCCGTTCAACTAGCAGACCATTATCAAC
AAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTAC
CTGTCGACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGCGTGACCA
CATGGTCCTTCTTGAGTTTGTAACTGCTGCTGGGATTACACATGGCATGG
ATGAGCTCTACAAACTCGAGCACCACCACCACCACCACTGA

Amino Acid Sequence

MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTT
GKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISF
KDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNV
YITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHY
LSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKLEHHHHHH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU42574
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 1) This construct is for validating the system for expressing Parkin pS65 in bacteria; ; 2) The backbone is PCRNT-TOPO-pLtetO tetR; ; 3) Cloning sites: KpnI/HidIII --> this leaves on a 6His Tag!!!; ; 4) The parent plasmid: pCR-BluntII