cDNA Clone Cullin3

• Plasmid: pFBDM-GST-TEV-Cullin3
• Parent Plasmid: pFBDM
• Expressed: GST-TEV Cullin 3
• Tag: GST
• Species: Human

Sequence of Insert

gtcgacatgtcccctatactaggttattggaaaattaagggccttgtgca
acccactcgacttcttttggaatatcttgaagaaaaatatgaagagcatt
tgtatgagcgcgatgaaggtgataaatggcgaaacaaaaagtttgaattg
ggtttggagtttcccaatcttccttattatattgatggtgatgttaaatt
aacacagtctatggccatcatacgttatatagctgacaagcacaacatgt
tgggtggttgtccaaaagagcgtgcagagatttcaatgcttgaaggagcg
gttttggatattagatacggtgtttcgagaattgcatatagtaaagactt
tgaaactctcaaagttgattttcttagcaagctacctgaaatgctgaaaa
tgttcgaagatcgtttatgtcataaaacatatttaaatggtgatcatgta
acccatcctgacttcatgttgtatgacgctcttgatgttgttttatacat
ggacccaatgtgcctggatgcgttcccaaaattagtttgttttaaaaaac
gtattgaagctatcccacaaattgataagtacttgaaatccagcaagtat
atagcatggcctttgcagggctggcaagccacgtttggtggtggcgacca
tcctccaaaatcggatggatccGAAAACCTGTATTTTCAGATGAGCAATC
TGAGCAAAGGCACCGGTTCCCGTAAAGACACCAAAATGCGTATCCGTGCG
TTCCCGATGACGATGGACGAAAAATATGTGAACAGCATTTGGGATCTGCT
GAAAAACGCGATTCAGGAAATCCAACGTAAAAACAATTCAGGTCTGTCGT
TTGAAGAACTGTATCGCAATGCCTACACGATGGTGCTGCATAAACACGGC
GAAAAACTGTATACCGGTCTGCGTGAAGTGGTTACGGAACATCTGATTAA
TAAAGTCCGCGAAGACGTGCTGAACAGCCTGAACAATAACTTTCTGCAGA
CCCTGAATCAAGCATGGAACGATCACCAGACGGCTATGGTGATGATTCGT
GACATCCTGATGTATATGGATCGCGTTTACGTCCAGCAAAATAACGTGGA
AAATGTTTATAACCTGGGTCTGATTATCTTCCGTGATCAGGTCGTGCGCT
ACGGCTGCATCCGTGACCATCTGCGCCAAACCCTGCTGGATATGATTGCC
CGTGAACGCAAAGGCGAAGTTGTGGATCGTGGTGCGATCCGCAACGCCTG
TCAGATGCTGATGATTCTGGGCCTGGAAGGTCGTTCAGTGTATGAAGAAG
ATTTCGAAGCGCCGTTTCTGGAAATGAGTGCCGAATTTTTCCAGATGGAA
TCCCAAAAATTTCTGGCAGAAAATAGTGCTTCCGTTTACATCAAAAAAGT
CGAAGCACGTATTAACGAAGAAATCGAACGCGTCATGCACTGCCTGGATA
AAAGCACCGAAGAACCGATTGTTAAAGTGGTTGAACGCGAACTGATCAGT
AAACACATGAAAACCATCGTTGAAATGGAAAATTCCGGCCTGGTCCACAT
GCTGAAAAACGGCAAAACCGAAGACCTGGGTTGTATGTATAAACTGTTTA
GTCGTGTGCCGAATGGTCTGAAAACCATGTGCGAATGTATGAGCAGCTAC
CTGCGCGAACAGGGCAAAGCACTGGTCTCCGAAGAAGGCGAAGGTAAAAA
CCCGGTGGATTATATCCAGGGTCTGCTGGACCTGAAATCACGTTTTGATC
GCTTCCTGCTGGAATCGTTCAATAACGACCGTCTGTTcAAgCAGACCATT
GCGGGCGATTTTGAATATTTCCTGAATCTGAACTCACGCTCGCCGGAATA
CCTGAGTCTGTTTATCGATGACAAACTGAAAAAAGGCGTGAAAGGTCTGA
CCGAACAGGAAGTTGAAACGATTCTGGACAAAGCAATGGTTCTGTTTCGT
TTCATGCAGGAAAAAGATGTCTTCGAACGCTATTACAAACAACATCTGGC
TCGTCGCCTGCTGACCAATAAAAGCGTGTCTGATGACAGCGAGAAAAACA
TGATCTCTAAACTGAAAACCGAATGCGGCTGTCAGTTCACGAGCAAACTG
GAAGGCATGTTTCGTGACATGAGTATTTCCAATACCACGATGGATGAATT
TCGCCAGCACCTGCAAGCGACCGGTGTGTCACTGGGCGGTGTTGATCTGA
CGGTGCGTGTTCTGACCACGGGTTATTGGCCGACCCAGTCGGCAACGCCG
AAATGCAACATCCCGCCGGCACCGCGTCATGCATTTGAAATTTTCCGTCG
CTTTTATCTGGCCAAACACTCAGGCCGCCAGCTGACCCTGCAACATCACA
TGGGTTCGGCAGACCTGAATGCTACGTTCTACGGCCCGGTGAAAAAAGAA
GATGGCAGCGAAGTCGGTGTGGGCGGTGCCCAGGTGACCGGTAGCAACAC
GCGTAAACATATCCTGCAGGTTTCTACCTTCCAAATGACGATTCTGATGC
TGTTTAATAACCGCGAAAAATACACCTTCGAAGAAATCCAGCAAGAAACG
GATATTCCGGAACGTGAACTGGTGCGCGCGCTGCAGAGCCTGGCATGTGG
TAAACCGACCCAACGCGTTCTGACGAAAGAACCGAAATCTAAAGAAATCG
AAAACGGCCATATCTTCACCGTGAACGACCAGTTTACGAGTAAACTGCAC
CGTGTCAAAATCCAGACCGTGGCGGCCAAACAGGGTGAATCCGATCCGGA
ACGTAAAGAAACGCGCCAGAAAGTGGATGACGATCGCAAACATGAAATTG
AAGCAGCTATTGTTCGTATCATGAAAAGCCGTAAGAAAATGCAGCACAAT
GTTCTGGTCGCAGAAGTTACCCAGCAACTGAAAGCTCGTTTTCTGCCGTC
TCCGGTCGTGATTAAAAAACGCATCGAAGGCCTGATTGAACGTGAATACC
TGGCTCGCACCCCGGAAGATCGTAAAGTCTACACCTATGTCGCCTGAgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDGSENLYFQMSNLSKGTGSRKDTKMRIRAFPMTMDEKYVNSIWDLLKNAIQEIQRKNNSGLSFEELYRNAYTMVLHKHGEKLYTGLREVVTEHLINKVREDVLNSLNNNFLQTLNQAWNDHQTAMVMIRDILMYMDRVYVQQNNVENVYNLGLIIFRDQVVRYGCIRDHLRQTLLDMIARERKGEVVDRGAIRNACQMLMILGLEGRSVYEEDFEAPFLEMSAEFFQMESQKFLAENSASVYIKKVEARINEEIERVMHCLDKSTEEPIVKVVERELISKHMKTIVEMENSGLVHMLKNGKTEDLGCMYKLFSRVPNGLKTMCECMSSYLREQGKALVSEEGEGKNPVDYIQGLLDLKSRFDRFLLESFNNDRLFKQTIAGDFEYFLNLNSRSPEYLSLFIDDKLKKGVKGLTEQEVETILDKAMVLFRFMQEKDVFERYYKQHLARRLLTNKSVSDDSEKNMISKLKTECGCQFTSKLEGMFRDMSISNTTMDEFRQHLQATGVSLGGVDLTVRVLTTGYWPTQSATPKCNIPPAPRHAFEIFRRFYLAKHSGRQLTLQHHMGSADLNATFYGPVKKEDGSEVGVGGAQVTGSNTRKHILQVSTFQMTILMLFNNREKYTFEEIQQETDIPERELVRALQSLACGKPTQRVLTKEPKSKEIENGHIFTVNDQFTSKLHRVKIQTVAAKQGESDPERKETRQKVDDDRKHEIEAAIVRIMKSRKKMQHNVLVAEVTQQLKARFLPSPVVIKKRIEGLIEREYLARTPEDRKVYTYVA*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU42585
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Knocked out PmeI restriction site (silent change) to enable further subcloning