cDNA Clone VAPB

• Synonyms: VAP-B , ALS8
• Plasmid: pET28a- VAPB aa1-210 -TEV-HALO (C ter)
• Parent Plasmid: pET28a C-TEV Halo1
• Expressed: VAPB (aa1-210) - HALO
• Genbank: NM_004738.3
• Tag: HALO
• Species: Human

Sequence of Insert

GGATCCATGGCGAAGGTGGAGCAGGTCCTGAGCCTCGAGCCGCAGCACGA
GCTCAAATTCCGAGGTCCCTTCACCGATGTTGTCACCACCAACCTAAAGC
TTGGCAACCCGACAGACCGAAATGTGTGTTTTAAGGTGAAGACTACAGCA
CCACGTAGGTACTGTGTGAGGCCCAACAGCGGAATCATCGATGCAGGGGC
CTCAATTAATGTATCTGTGATGTTACAGCCTTTCGATTATGATCCCAATG
AGAAAAGTAAACACAAGTTTATGGTTCAGTCTATGTTTGCTCCAACTGAC
ACTTCAGATATGGAAGCAGTATGGAAGGAGGCAAAACCGGAAGACCTTAT
GGATTCAAAACTTAGATGTGTGTTTGAATTGCCAGCAGAGAATGATAAAC
CACATGATGTAGAAATAAATAAAATTATATCCACAACTGCATCAAAGACA
GAAACACCAATAGTGTCTAAGTCTCTGAGTTCTTCTTTGGATGACACCGA
AGTTAAGAAGGTTATGGAAGAATGTAAGAGGCTGCAAGGTGAAGTTCAGA
GGCTACGGGAGGAGAACAAGCAGTTCAAGGAAGAAGATGGACTGCGGATG
AGGAAGACAGTGCAGAGCAACAGCCCCATTTCAGCAGAATTC

Amino Acid Sequence

MAKVEQVLSLEPQHELKFRGPFTDVVTTNLKLGNPTDRNVCFKVKTTAPR
RYCVRPNSGIIDAGASINVSVMLQPFDYDPNEKSKHKFMVQSMFAPTDTS
DMEAVWKEAKPEDLMDSKLRCVFELPAENDKPHDVEINKIISTTASKTET
PIVSKSLSSSLDDTEVKKVMEECKRLQGEVQRLREENKQFKEEDGLRMRK
TVQSNSPISAEFELRRQASENLYFQGMAEIGTGFPFDPHYVEVLGERMHY
VDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDK
PDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVK
GIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLP
MGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLL
QEDNPDLIGSEIARWLSTLEISG*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU23949
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: NO N-terminal 6HIS tag, TEV not blunt; Parental 23919