cDNA Clone VAPB

• Synonyms: VAP-B , ALS8
• Plasmid: pET28a- VAPB aa1-210 K87D M89D -TEV-HALO (C ter)
• Parent Plasmid: pET28a C-TEV Halo1
• Expressed: VAPB (aa1-210 K87D M89D) -HALO
• Genbank: NM_004738.3
• Tag: HALO
• Species: Human

Sequence of Insert

GGATCCATGGCGAAGGTGGAGCAGGTCCTGAGCCTCGAGCCGCAGCACGA
GCTCAAATTCCGAGGTCCCTTCACCGATGTTGTCACCACCAACCTAAAGC
TTGGCAACCCGACAGACCGAAATGTGTGTTTTAAGGTGAAGACTACAGCA
CCACGTAGGTACTGTGTGAGGCCCAACAGCGGAATCATCGATGCAGGGGC
CTCAATTAATGTATCTGTGATGTTACAGCCTTTCGATTATGATCCCAATG
AGAAAAGTAAACACGACTTTGACGTTCAGTCTATGTTTGCTCCAACTGAC
ACTTCAGATATGGAAGCAGTATGGAAGGAGGCAAAACCGGAAGACCTTAT
GGATTCAAAACTTAGATGTGTGTTTGAATTGCCAGCAGAGAATGATAAAC
CACATGATGTAGAAATAAATAAAATTATATCCACAACTGCATCAAAGACA
GAAACACCAATAGTGTCTAAGTCTCTGAGTTCTTCTTTGGATGACACCGA
AGTTAAGAAGGTTATGGAAGAATGTAAGAGGCTGCAAGGTGAAGTTCAGA
GGCTACGGGAGGAGAACAAGCAGTTCAAGGAAGAAGATGGACTGCGGATG
AGGAAGACAGTGCAGAGCAACAGCCCCATTTCAGCAGAATTC

Amino Acid Sequence

MAKVEQVLSLEPQHELKFRGPFTDVVTTNLKLGNPTDRNVCFKVKTTAPR
RYCVRPNSGIIDAGASINVSVMLQPFDYDPNEKSKHDFDVQSMFAPTDTS
DMEAVWKEAKPEDLMDSKLRCVFELPAENDKPHDVEINKIISTTASKTET
PIVSKSLSSSLDDTEVKKVMEECKRLQGEVQRLREENKQFKEEDGLRMRK
TVQSNSPISAEFELRRQASENLYFQGMAEIGTGFPFDPHYVEVLGERMHY
VDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDK
PDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVK
GIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLP
MGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLL
QEDNPDLIGSEIARWLSTLEISG*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU23950
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: no N-ter 6His tag, TEV not blunt; Parental 23919