cDNA Clone VPS16A

• Plasmid: pBabe.puro.GFP.DU VPS16A
• Parent Plasmid: pBabeD puro GFP
• Expressed: GFP VPS16A
• Genbank: NM_022575.2 GI:17978478 with silent change to kill internal BglII
• Tag: GFP
• Species: Human

Sequence of Insert

AGATCTATGGACTGCTACACGGCGAACTGGAACCCACTCGGGGACTCTGCCTTTTACCGGAAATATGAGCTGTACAGCATGGACTGGGACCTGAA
GGAGGAACTCAGGGATTGCCTGGTGGCTGCTGCACCCTATGGGGGCCCCATTGCACTGCTGAGGAACCCCTGGCGGAAGGAGAAAGCTGCTAGTGTGAGG
CCAGTGCTCGATATATACTCTGCTTCCGGCATGCCTCTGGCCAGCCTGCTGTGGAAGAGTGGACCCGTGGTGTCCCTGGGCTGGTCAGCTGAGGAGGAGC
TGCTCTGTGTGCAGGAAGATGGTGCTGTACTGGTTTATGGGCTTCATGGTGACTTCCGGAGACACTTCAGCATGGGCAATGAAGTGCTCCAGAACCGGGT
TCTGGATGCCCGGATCTTTCACACTGAGTTTGGTTCCGGAGTGGCCATCCTCACAGGGGCCCACCGCTTCACCCTCAGTGCCAATGTGGGTGACCTCAAA
CTCCGCCGGATGCCAGAGGTGCCAGGTCTGCAAAGTGCACCCTCCTGCTGGACTGTGCTGTGCCAGGACCGAGTGGCACACATTCTTCTGGCTGTGGGGC
CTGACCTTTACCTCTTGGACCATGCAGCCTGCTCCGCAGTGACGCCCCCTGGCCTGGCCCCAGGAGTAAGCAGCTTCCTACAGATGGCTGTCTCCTTCAC
CTACCGACACCTGGCACTCTTCACAGACACAGGCTACATCTGGATGGGGACAGCATCACTCAAGGAGAAGCTATGTGAGTTCAACTGCAACATCCGGGCA
CCTCCAAAGCAGATGGTCTGGTGCAGCCGTCCTCGTAGCAAGGAGAGGGCCGTGGTGGTGGCCTGGGAAAGGCGGCTGATGGTGGTGGGCGATGCACCCG
AGAGCATCCAGTTTGTGCTGGATGAGGACTCCTACCTGGTGCCTGAGCTCGATGGGGTCCGCATCTTCTCCCGCAGCACCCACGAGTTCCTGCATGAGGT
TCCAGCGGCCAGCGAGGAAATCTTCAAAATTGCCTCAATGGCCCCCGGGGCGCTGCTCCTGGAGGCTCAGAAGGAGTATGAGAAAGAGAGCCAGAAGGCG
GACGAGTACCTGCGGGAGATCCAGGAGCTGGGCCAGCTGACCCAGGCCGTGCAGCAGTGCATTGAGGCTGCAGGACATGAGCACCAGCCAGACATGCAGA
AGAGTCTGCTCAGGGCCGCCTCCTTCGGAAAGTGTTTCCTGGACAGATTTCCACCCGACAGCTTCGTGCACATGTGTCAGGACCTGCGTGTGCTCAATGC
TGTTCGGGACTATCACATCGGGATCCCGCTCACCTATAGCCAATATAAGCAGCTCACCATCCAGGTGCTGCTGGACAGGCTCGTGTTGCGGAGACTTTAC
CCCCTGGCCATCCAGATATGCGAGTACTTGCGCCTTCCTGAAGTACAGGGCGTCAGCAGGATCCTGGCCCACTGGGCCTGCTACAAGGTGCAACAGAAGG
ATGTCTCAGATGAGGATGTGGCTCGAGCCATTAACCAGAAGCTGGGGGACACGCCTGGTGTCTCTTACTCCGACATTGCTGCACGAGCCTATGGTTGTGG
CCGCACGGAGCTGGCCATCAAGCTGCTGGAGTATGAGCCACGCTCAGGGGAGCAGGTACCCCTTCTCCTAAAGATGAAGAGGAGCAAACTGGCACTAAGC
AAGGCCATCGAGAGCGGGGACACTGACCTGGTGTTCACGGTGTTGCTGCACCTGAAGAACGAGCTGAACCGAGGAGATTTTTTCATGACCCTTCGGAATC
AGCCCATGGCCCTCAGTTTGTACCGACAGTTCTGTAAGCATCAGGAGCTAGAGACGCTGAAGGACCTTTACAATCAGGATGACAATCACCAGGAATTGGG
CAGCTTCCACATCCGAGCCAGCTATGCTGCAGAAGAGCGTATTGAGGGGCGAGTAGCAGCTCTGCAGACAGCCGCCGATGCCTTCTACAAGGCCAAGAAT
GAGTTTGCAGCCAAGGCTACAGAGGATCAAATGCGGCTCCTACGGCTGCAGCGGCGCCTAGAAGACGAGCTGGGGGGCCAGTTCCTAGACCTGTCTCTAC
ATGACACAGTTACCACCCTCATTCTTGGCGGTCACAACAAGCGTGCAGAGCAGCTGGCACGTGACTTCCGCATCCCTGACAAGAGGCTCTGGTGGCTGAA
GCTGACTGCCCTGGCAGATTTGGAAGATTGGGAAGAGCTAGAGAAGTTTTCCAAGAGCAAGAAATCACCCATTGGCTACCTGCCTTTTGTGGAAATCTGC
ATGAAACAACATAACAAATACGAAGCCAAGAAGTATGCTTCCCGCGTGGGTCCCGAGCAGAAGGTCAAGGCTTTGCTTCTTGTTGGCGATGTGGCTCAGG
CTGCAGATGTGGCCATCGAACACCGGAATGAGGCTGAGCTGAGCCTCGTATTGTCCCACTGCACGGGAGCCACAGATGGGGCCACAGCTGACAAGATTCA
ACGGGCCAGGGCACAAGCCCAGAAGAAGTGAGCGGCCGC

Amino Acid Sequence

MHVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFIC
TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMDCYT
ANWNPLGDSAFYRKYELYSMDWDLKEELRDCLVAAAPYGGPIALLRNPWR
KEKAASVRPVLDIYSASGMPLASLLWKSGPVVSLGWSAEEELLCVQEDGA
VLVYGLHGDFRRHFSMGNEVLQNRVLDARIFHTEFGSGVAILTGAHRFTL
SANVGDLKLRRMPEVPGLQSAPSCWTVLCQDRVAHILLAVGPDLYLLDHA
ACSAVTPPGLAPGVSSFLQMAVSFTYRHLALFTDTGYIWMGTASLKEKLC
EFNCNIRAPPKQMVWCSRPRSKERAVVVAWERRLMVVGDAPESIQFVLDE
DSYLVPELDGVRIFSRSTHEFLHEVPAASEEIFKIASMAPGALLLEAQKE
YEKESQKADEYLREIQELGQLTQAVQQCIEAAGHEHQPDMQKSLLRAASF
GKCFLDRFPPDSFVHMCQDLRVLNAVRDYHIGIPLTYSQYKQLTIQVLLD
RLVLRRLYPLAIQICEYLRLPEVQGVSRILAHWACYKVQQKDVSDEDVAR
AINQKLGDTPGVSYSDIAARAYGCGRTELAIKLLEYEPRSGEQVPLLLKM
KRSKLALSKAIESGDTDLVFTVLLHLKNELNRGDFFMTLRNQPMALSLYR
QFCKHQELETLKDLYNQDDNHQELGSFHIRASYAAEERIEGRVAALQTAA
DAFYKAKNEFAAKATEDQMRLLRLQRRLEDELGGQFLDLSLHDTVTTLIL
GGHNKRAEQLARDFRIPDKRLWWLKLTALADLEDWEELEKFSKSKKSPIG
YLPFVEICMKQHNKYEAKKYASRVGPEQKVKALLLVGDVAQAADVAIEHR
NEAELSLVLSHCTGATDGATADKIQRARAQAQKK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU45478
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee