cDNA Clone SPAK/MO25

• Plasmid: pFBDM MO25/GST-SPAK
• Parent Plasmid: pFBDM
• Expressed: MO25
• Genbank: NM_016289 with silent change c243t
• Tag: untagged
• Species: Human

Sequence of Insert

CTCGAGCGGCCGCCAGTGTGATGGATATCTGCAGAATTCGCCCTTGAAACCATGCCGTTCCCGTTTGGGAAGTCTCACAAATCTCCAGCAGACATTGTGAAGAATCTGAAGGAGAGCATGGCTGTTCTGGAAAAGCAAGACATTTCTGATAAAAAAGCAGAAAAGGCTACAGAAGAAGTTTCCAAAAATCTGGTTGCCATGAAAGAAATTCTGTATGGCACAAATGAAAAAGAGCCTCAGACAGAAGCAGTAGCTCAACTTGCTCAAGAACTCTATAATAGTGGGCTCCTTAGTACCCTGGTAGCTGATTTACAGCTCATTGACTTTGAGGGCAAAAAAGACGTGGCTCAAATTTTCAACAATATTCTCAGAAGACAAATTGGTACGAGAACTCCTACTGTTGAATACATCTGCACCCAACAGAATATTTTGTTCATGTTATTGAAAGGGTATGAATCTCCAGAAATAGCTCTAAATTGTGGAATAATGTTAAGAGAATGCATCAGACATGAACCACTTGCAAAAATCATTTTGTGGTCGGAACAGTTTTATGATTTCTTCAGATATGTCGAAATGTCAACATTTGACATAGCTTCAGATGCATTTGCCACATTCAAGGATTTACTTACAAGACATAAATTGCTCAGTGCAGAATTTTTGGAACAGCATTATGATAGATTTTTCAGTGAATATGAGAAGTTACTTCATTCAGAAAATTATGTGACAAAAAGACAGTCACTGAAGCTTCTCGGTGAACTACTACTAGATAGACACAACTTCACAATTATGACAAAATACATCAGTAAACCTGAGAACCTCAAATTAATGATGAACCTGCTGCGAGACAAAAGTCGCAACATCCAGTTTGAGGCCTTTCACGTTTTTAAGGTGTTTGTAGCCAATCCTAACAAGACGCAGCCCATCCTAGACATCCTCCTCAAGAACCAGGCCAAACTCATAGAGTTCCTCAGCAAGTTTCAGAACGACAGGACGGAGGATGAGCAGTTTAACGACGAGAAGACCTATTTAGTTAAACAGATCAGGGATTTGAAGAGACCAGCTCAGCAAGAAGCTTAAGGATCCAAGGGCGAATTCCAGCACACTGGCGGCCGTTACTAGTGGATCCGAGCTCGGTACC

Amino Acid Sequence

MPFPFGKSHKSPADIVKNLKESMAVLEKQDISDKKAEKATEEVSKNLVAM
KEILYGTNEKEPQTEAVAQLAQELYNSGLLSTLVADLQLIDFEGKKDVAQ
IFNNILRRQIGTRTPTVEYICTQQNILFMLLKGYESPEIALNCGIMLREC
IRHEPLAKIILWSEQFYDFFRYVEMSTFDIASDAFATFKDLLTRHKLLSA
EFLEQHYDRFFSEYEKLLHSENYVTKRQSLKLLGELLLDRHNFTIMTKYI
SKPENLKLMMNLLRDKSRNIQFEAFHVFKVFVANPNKTQPILDILLKNQA
KLIEFLSKFQNDRTEDEQFNDEKTYLVKQIRDLKRPAQQEA*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU45536
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Cloned MO25 from DU2051 Xho1/Kpn1 into DU41860 (pFBDM GST-SPAK). ; N.B. Xho1/Kpn1 insert from DU2051 has extra seq up and downstream of ORF so beware additional restriction sites if using to combine with other pFBDM clones.; ; All SPAK plasmids MUST be grown at 30 degrees C or less to prevent recombination.