cDNA Clone KIAA2022

• Plasmid: pcDNA5 FRT/TO GFP-NLS KIAA2022 L281E V283E L285E
• Parent Plasmid: pcDNA5 FRT/TO GFP NLS
• Expressed: GFP-NLS-KIAA2022 L281E V283E L285E
• Genbank: NM_001008537.2 with silent changes to kill internal BamH1 sites
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGGATAACCAACAAGATAAGGCTATTGTTGCCTCAGCCAACGGAGAAAACACTCTGATTAATGGGGTCAAAGAAAATGACTCAGAGGACCAGGATGTGGCAATGAAGTCATTTGCAGCTCTAGAAGCTGCTGCACCTATCCAGCCTACACCGGTGGCACAAAAAGAGACCCTGATGTATCCCAGAGGTCTCCTGCCTCTACCCTCTAAGAAGCCCTGTATGCAGAGCCCGCCCTCTCCTTTGGGCCTGATTGAAGCACCCGAACATGCTGCTAATAGTGCTTCTGTGAATGCCATCTCCCTCACATCTGGCATTGCAAAAGGCCTGAACACATGGTCACTTCCCAATGAATGTGAGAAAGCTCCATTTGCCATAATGGAGCCTGCAGGCATGTCAGCTCTGAATGGGGACTGTCTCATGCAGCCAAGTCGGACTTGCTTAGGCTGCTTCATGGAATCCAAGGATGCAGTAGATCCTGAGCCAGGGATCAGTCTGAAAGTTGGTGATCTAAATAGGGATTATGAAACGTGTGCAGTCTCTGATATAGGGATTCAGTGTATTAATGCTGGAGAAAATATGAAATATGGAGAGCAGCTGCTCTCAGACCAGCTCCTAGGCTTCCCCCTGCATAAGTCAAGGGCAGGAGACAGACGAGAAACTGAGAAACCTGACATTGACTTGGAGGACCCGGCTCAGAAAAGCTATTATGAGGCATTACTGTTAGACAAGTGCAATACAGAAGAAGCTTTGCTTGCAAATTCCAATCAGGATTGGGGTTACTTCGAGACTTTTATTAGTGAAAGTAAGATTGAACTGCTTGACCTCTGTTCCAAAAATGAGGAGTCTGAGAACGAGTTCTCTGAAGAAGATGTGGATAACTACATGTTTGATGATGATGAATCAACACTAGGCAGTGATGTCTGCTCCCTGAAAATTCGATATGAATCCTTTCAGGACAATGTTCGAGACAAGACTACTCTTTTGATGCAGGAAGATGCCCAATTCAACTTTTTTCCCAGCGTCTTTACTACCTGCCCCAAGCGAGAGTCTAAGAGTGGGGCCCTGAAGCAGAGCAGTGATTTTTCCCAATTCAAGGTCCCTGATGTGAGCATCATCTGGGGGGAGGAAGATAAAAACTTGGACAAGAAGAAAGGCAAAGAGGAAGGACAGGAAGACAAAGGTGTAGAGAAGAAAGATGGAAAGGATAATGGAGAAAAGCCTGCCTTAAATAAACCATGCAGTGGGACTGAAGTAGAGCAACTTAAGAATCCAAAGCAGGGCCATCTTGCTAATTCCCTGGAGACATCAGGGAGTTTCAGTGATGATAGTTCCTTCATTGAGATCTCATATGATGCTATGGGTGAGATCAAGGACTGTAGTCGCTATATGGCTCGGGACACTAATTCTGGCAGCTCCTCCTCCCAACAGAACTATGGGCTGCGAGCCAAGAGAAAAGTCAGATACAGTGAAGATTATCTATATGATGTTGACTCACTAGAGGGTGAAAAAGTAAATGAGAGGAAGGAATGGCTGCCAGTTGGTTCCAAAGAGGAAGATGATGATGAATGGTGTCCCAAAAAGAGAAGAAAAGTAACCCGTAAGGAGCCCCCTGTTATTATCAAATATATCATCATTAATCGCTTTAAAGGTGAGAAGAACATGCTGGTGAAGTTGGGTAAGGTGGATGCCAGTGAGACAACAGTGAATTTGAGTGAGAATCAGCTCAACAAATATGCCAAGCTGGCACCCTTGAAAGGCTTCTGGCAAAAGAAGAAGAAGCAGAGAAACACCAACACGGACTCCATCAAGACACCTTTTTCCCAAAAGCAAAGCTTTGAACCAGGTAGCTTTGAGGTGTCATTTCTGCCACCTGCTCGCAAACGAAAATCTAAACTTGGCAACAGGCACAGGATTCAAAGAATCCCATCCATTGAAATTTCAGCAAGTAGTAAACAGATTTCATTATGTAATGATCAGAGGCACGCTAGTAATCATAAAGAAGATGGAGGCCTAAAAGGTACACTGAAGTCAGCACCTCTGGGTGCTCCTAGCTGTGCAAATGGATCACATTTAAATGACATCACAGGCCCTGACTCAGTGAAAGTCAAAGCCCAAGACACAGAGTTTAAGGGGCCAGAGAGGAAAGTGCTCAATAAAATCAAATTTAAAAGTGAAGCTAGGTTAAAATCTAAGAAAGTCAAAGCTGCTGGGCAAGAAAGCAAGCCAATTGTTCAAATGAGCCCTCTCTTGGAAAACCAATCCTCAAAGGCTAATTTAAAGAATGAAGTTATTCCTGGGACATCAAACAGTTCCCGTCTATCTGAATTTCATGAGGCAAAGGCTGCTAAGAGTTCCACTTTTCTACCAACGACATGCTCTTCTGAAATGCCTTTATCATCTGCTAATGTTACCACTAATATACCTGTTATCCCGGGAGGGTATCTGCAGACATTGTTAGATGCTTCTGACTTGTCAAATAACACTAGTATCTCATACTTCAGCCACCATTCTCCAGAGCAAAATGAAGGCAGCCTCACTCAAACCGAAAAATCATTTGTACCCCTCCAGCCTACCCAGGACTGTGTGCTCACCTCATCCTCTGACTCTGAGCTGCAGCAGTCATCTCATAACTTCAAAATGGAATCAAGCAACTATAGAAATGTGTGGCCCAACAAGGCTACTTCTGGCACCCAGGAATTCATGGCTGAAGTCTCAAGGGAGATAGCCCCAACCCAATCCAGTGAATTTGGAGCCTCCCAAGTAGTCTCCATGGAAAATAACCTCACACCTACAACATACAATCCAATCTGTCTCAATAGTGGTGGCAGTAATTGCAACAAGGTCCTGTATGACTCCATGCAAGATACCCAACTCCCATCTGATGACTCTTACCAATTATGTCACTTTAATAATGGAGAGATCTGCTTTCCTTTCCAGCAGGGGCCAGTCAATATGGATGATGGTCGGCTCTTTAGCTTTGATTCAATGGCCCCACTCTCTGTCAGCTCAAGTAATTATTGCTCCTTAAGCTTGAAGTCCTGTGAAAAGGATGGCGATGATGATATCACTGATGACTTCCTGGCCCATTGCAGCCCTAAGCTGGTGATCCAGCAAAGCATTGATGAGATAGCACCACTAAAGGAGTCCACTGACCTCCTGGATATATCCAACTTCACCCCTGACAAATTCCGCCACTCTTCCCTTTCAGAGATGTCCCCACCGGACACCCCTAGTCTTTCCCCTCAAATTACCAGATGTGAGAGTATGAAGACACTAGGAACACTAAAGGGGTTCCAAGAGGGTGTCCCAGGACCATTGGACAGTGTGGAAAAAATCAAGTGGGACTGCAGTACCCTTTCACGGCAGGTCCAAATGGAGGATGGATTTACTTTAAATAATCACCAGTTTCAGTTCCATATGTTCAATGATGAGGATTCTGTCAGCCTGCTCCAAAAAAACCCTTGCCTGTCAACATTTAATGATCCATCTGGTCAAATTAGTACCAACAACAAAGTGTCAAAATCAAGAAAGAAAAGTTCACCCAGCAAGAGTGGGGCTATGAACCAAAGCTCTTCTCAGAAAAACACCAGGAAAAAATCCCTCAAAGGTAACAACAAGGGGATTGAAAAACCACCTGGCAAAAACTCCCGCCAGGTCCCTAAGTCCACAAAGAAAGGGAAATACATGGCTGCCATCAATGGAGAGAAAATGCAAATTGGCATTGGCCGTGGGGGAAGCCAAACCAACACCATATCCTCCACTGGGAAGACATTGGCTGAATGTATCCAACATGGTGGCCCTATGGCCTCTATGAAGATGCCAAGTCAAAAGGGACTTTCTGGAGATTGGGCCTTGGGGAAGGAGAGCAGCCCAGGCTGGAGTGATATGAGCATGGGCACCAACACCAACAGCCTTCTGGATGATGACCAACGGGAATTTCAGGAGCCTTCCTATATCTTGTCCAACATTGCCTCTGGTATGGCAGATGTGCAGAGATTCATGATGGCCTCCATAGAGCCCCTTTGGGAACCCATGGAGCACCATGGAGATCCCAACATATTCTACTCCCCTGAGTCCAATAGTCTAAAATTAAAAACCCTCAAAATATTGGCTGGGACACCACAGGAGTCTAAGAAAAAGATCAACAGTGGGTCCCAAGGAGCCACTAAGAATCACAGGTCAATCAAGGGTGTGAGCAAAAGCAATGGGAAAACAGCAATAGGTGATCCTGGTCGTGCAAACATGCCTGGTTATAACGAGGACTCTCGCTCTACCTTCTTTGATAAAAAGTATAGTAACATGAGCACTTTAGGCAATAACGGACCGACACACAAAAAGTTGTATCGTCACAAATCCAGCTCCAAGGCCCTGAGAGATGAGAAATGTAAGGGAAAGCACATGGAGCGAGAACAGGTCCACAAGGATGAGTCTGGGACAGCTTCTTTTGAAAAACTGAGGGATTCCGACTACAATCTCCTAAAAGCAGAAACAACCTTTTGGGTTTTACCTGTGTTTGAAGAAGAGACTCGCATTTTCCAGAAAGACATTTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLPPKKKRKV
GSMDNQQDKAIVASANGENTLINGVKENDSEDQDVAMKSFAALEAAAPIQ
PTPVAQKETLMYPRGLLPLPSKKPCMQSPPSPLGLIEAPEHAANSASVNA
ISLTSGIAKGLNTWSLPNECEKAPFAIMEPAGMSALNGDCLMQPSRTCLG
CFMESKDAVDPEPGISLKVGDLNRDYETCAVSDIGIQCINAGENMKYGEQ
LLSDQLLGFPLHKSRAGDRRETEKPDIDLEDPAQKSYYEALLLDKCNTEE
ALLANSNQDWGYFETFISESKIELLDLCSKNEESENEFSEEDVDNYMFDD
DESTLGSDVCSLKIRYESFQDNVRDKTTLLMQEDAQFNFFPSVFTTCPKR
ESKSGALKQSSDFSQFKVPDVSIIWGEEDKNLDKKKGKEEGQEDKGVEKK
DGKDNGEKPALNKPCSGTEVEQLKNPKQGHLANSLETSGSFSDDSSFIEI
SYDAMGEIKDCSRYMARDTNSGSSSSQQNYGLRAKRKVRYSEDYLYDVDS
LEGEKVNERKEWLPVGSKEEDDDEWCPKKRRKVTRKEPPVIIKYIIINRF
KGEKNMLVKLGKVDASETTVNLSENQLNKYAKLAPLKGFWQKKKKQRNTN
TDSIKTPFSQKQSFEPGSFEVSFLPPARKRKSKLGNRHRIQRIPSIEISA
SSKQISLCNDQRHASNHKEDGGLKGTLKSAPLGAPSCANGSHLNDITGPD
SVKVKAQDTEFKGPERKVLNKIKFKSEARLKSKKVKAAGQESKPIVQMSP
LLENQSSKANLKNEVIPGTSNSSRLSEFHEAKAAKSSTFLPTTCSSEMPL
SSANVTTNIPVIPGGYLQTLLDASDLSNNTSISYFSHHSPEQNEGSLTQT
EKSFVPLQPTQDCVLTSSSDSELQQSSHNFKMESSNYRNVWPNKATSGTQ
EFMAEVSREIAPTQSSEFGASQVVSMENNLTPTTYNPICLNSGGSNCNKV
LYDSMQDTQLPSDDSYQLCHFNNGEICFPFQQGPVNMDDGRLFSFDSMAP
LSVSSSNYCSLSLKSCEKDGDDDITDDFLAHCSPKLVIQQSIDEIAPLKE
STDLLDISNFTPDKFRHSSLSEMSPPDTPSLSPQITRCESMKTLGTLKGF
QEGVPGPLDSVEKIKWDCSTLSRQVQMEDGFTLNNHQFQFHMFNDEDSVS
LLQKNPCLSTFNDPSGQISTNNKVSKSRKKSSPSKSGAMNQSSSQKNTRK
KSLKGNNKGIEKPPGKNSRQVPKSTKKGKYMAAINGEKMQIGIGRGGSQT
NTISSTGKTLAECIQHGGPMASMKMPSQKGLSGDWALGKESSPGWSDMSM
GTNTNSLLDDDQREFQEPSYILSNIASGMADVQRFMMASIEPLWEPMEHH
GDPNIFYSPESNSLKLKTLKILAGTPQESKKKINSGSQGATKNHRSIKGV
SKSNGKTAIGDPGRANMPGYNEDSRSTFFDKKYSNMSTLGNNGPTHKKLY
RHKSSSKALRDEKCKGKHMEREQVHKDESGTASFEKLRDSDYNLLKAETT
FWVLPVFEEETRIFQKDI*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU45573
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee