cDNA Clone TTBK2

• Synonyms: KIAA0847, SCA11, TTBK
• Plasmid: pcDNA5frtTO HALO TTBK2
• Parent Plasmid: pcDNA5 FRT/TO Halo
• Expressed: HALO-Prec-TTBK2
• Genbank: NM_173500.3
• Tag: HALO
• Species: Human

Sequence of Insert

GGATCCGCCATGAGTGGGGGAGGAGAGCAGCCGGATATCCTGAGTGTTGGAATCCTAGTGAAAGAAAGATGGAAAGTGTTGAGAAAGATTGGGGGTGGGGGCTTTGGAGAAATTTACGATGCCTTGGACATGCTCACCAGGGAAAATGTTGCACTGAAGGTGGAATCAGCTCAACAACCAAAACAAGTTCTGAAAATGGAAGTTGCTGTTTTGAAAAAGCTGCAAGGGAAAGACCATGTTTGTAGATTTATTGGCTGTGGGAGGAATGATCGATTCAACTATGTGGTCATGCAGTTGCAGGGTCGGAATCTGGCAGATCTTCGCCGTAGCCAGTCCCGAGGCACATTCACCATTAGTACCACTCTCCGGCTGGGTAGACAGATTTTGGAGTCTATTGAAAGCATTCATTCTGTGGGATTCTTGCATCGAGACATCAAACCGTCGAACTTCGCTATGGGTCGCTTTCCTAGTACATGTAGGAAATGTTACATGCTTGATTTTGGCTTGGCTCGACAATTTACCAATTCCTGTGGTGACGTCAGACCACCTCGAGCTGTGGCAGGTTTTCGAGGGACAGTTCGTTATGCATCAATCAACGCACATCGGAACAGGGAAATGGGAAGACATGATGACCTTTGGTCCTTATTCTACATGTTGGTGGAGTTTGTGGTTGGTCAGCTGCCCTGGAGAAAAATAAAGGACAAGGAGCAAGTAGGCTCTATTAAGGAGAGATATGACCACAGGCTCATGTTGAAACATCTCCCTCCAGAATTCAGCATCTTTCTAGACCATATCTCTTCTTTGGATTATTTTACAAAACCAGACTACCAGCTTCTTACATCCGTGTTTGACAATAGCATCAAGACTTTTGGAGTAATTGAGAGTGACCCTTTTGACTGGGAGAAGACTGGAAATGATGGCTCCCTAACAACCACCACTACTTCTACCACCCCTCAGTTGCACACTCGCTTGACCCCTGCTGCAATTGGAATTGCCAATGCTACTCCCATCCCTGGAGACTTGCTTCGAGAAAATACAGATGAGGTATTTCCAGATGAACAGCTTAGCGATGGAGAAAATGGCATCCCTGTTGGTGTGTCACCAGATAAATTGCCTGGATCTCTGGGACACCCCCGTCCCCAGGAGAAGGATGTTTGGGAAGAGATGGATGCCAACAAAAACAAGATAAAGCTTGGAATTTGTAAGGCTGCTACTGAAGAGGAGAACAGCCATGGCCAGGCAAATGGTCTTCTCAATGCTCCAAGCCTTGGGTCACCAATTCGTGTCCGCTCAGAGATTACTCAGCCAGACAGAGATATTCCACTGGTGCGAAAGTTACGTTCCATTCACAGCTTTGAGCTGGAAAAACGTCTGACCCTGGAGCCAAAGCCAGACACTGACAAGTTCCTTGAGACCTGCCTGGAGAAAATGCAGAAAGATACCAGTGCAGGAAAAGAATCTATTCTCCCTGCTCTGCTGCATAAGCCTTGCGTTCCTGCTGTGTCCCGTACTGACCACATCTGGCACTATGATGAAGAATATCTTCCAGATGCCTCCAAGCCTGCTTCTGCCAACACCCCTGAGCAGGCAGATGGTGGTGGCAGCAATGGATTTATAGCTGTTAACCTGAGCTCTTGCAAGCAAGAAATTGATTCCAAAGAATGGGTGATTGTGGACAAGGAGCAGGACCTTCAGGATTTTAGGACAAATGAGGCTGTAGGACATAAAACAACTGGAAGTCCTTCTGATGAGGAGCCTGAAGTACTTCAAGTCCTGGAGGCATCACCTCAAGATGAAAAGCTCCAGTTAGGTCCTTGGGCAGAAAATGATCATTTAAAGAAGGAAACCTCAGGTGTGGTCTTAGCACTTTCTGCAGAGGGTCCTCCTACTGCTGCTTCAGAACAATATACAGATAGGCTGGAACTCCAGCCTGGAGCTGCTAGTCAGTTTATTGCAGCGACGCCCACAAGTCTAATGGAGGCGCAGGCAGAAGGACCCCTTACAGCGATTACAATTCCTAGACCTTCTGTGGCATCTACACAGTCAACTTCAGGAAGCTTTCACTGTGGTCAGCAGCCAGAGAAGAAAGATCTTCAGCCCATGGAGCCCACTGTGGAACTTTACTCTCCAAGGGAAAACTTCTCTGGCTTGGTTGTGACAGAGGGTGAACCTCCTAGTGGAGGAAGCAGAACAGATTTGGGGCTTCAGATAGATCACATTGGTCATGACATGTTACCCAACATTAGAGAAAGTAACAAATCTCAAGACCTGGGACCAAAAGAACTTCCTGATCATAATAGACTGGTTGTGAGAGAATTTGAAAATCTCCCTGGGGAAACTGAAGAGAAAAGCATCCTTTTAGAGTCAGATAATGAAGATGAGAAGTTAAGTAGAGGGCAGCATTGTATTGAGATCTCCTCTCTCCCAGGAGATTTGGTAATTGTGGAAAAGGATCACTCAGCTACTACTGAACCTCTTGATGTGACAAAAACACAGACTTTTAGTGTGGTGCCAAATCAAGACAAAAATAATGAGATAATGAAGCTTCTGACAGTTGGAACTTCAGAAATTTCTTCCAGAGACATTGACCCACATGTTGAAGGTCAGATAGGCCAAGTGGCAGAAATGCAAAAAAATAAGATATCTAAGGATGATGACATCATGAGTGAAGACTTGCCAGGTCATCAAGGAGACCTCTCTACTTTTTTGCACCAAGAGGGCAAGAGAGAGAAAATCACCCCTAGAAATGGAGAACTATTTCATTGTGTTTCAGAGAATGAACATGGTGCCCCAACCCGGAAGGATATGGTTAGGTCATCCTTTGTAACTAGACACAGCCGAATCCCTGTTTTAGCACAAGAGATAGACTCAACTTTGGAATCATCCTCTCCAGTTTCTGCAAAAGAAAAGCTCCTCCAAAAGAAAGCCTATCAGCCAGACCTAGTCAAGCTTCTGGTGGAAAAAAGACAATTCAAGTCCTTCCTTGGCGACCTCTCAAGTGCCTCTGATAAATTGCTAGAGGAGAAACTAGCTACTGTTCCTGCTCCCTTTTGTGAGGAGGAAGTGCTCACTCCCTTTTCAAGACTGACAGTAGATTCTCACCTGAGTAGGTCAGCTGAAGATAGCTTTCTGTCACCCATCATCTCCCAGTCTAGAAAGAGCAAAATTCCAAGGCCAGTTTCATGGGTCAACACAGATCAGGTCAATAGCTCAACTTCGTCTCAGTTCTTTCCTCGGCCACCACCAGGAAAGCCACCCACGAGGCCTGGAGTAGAAGCCAGGCTACGCAGATATAAAGTCCTAGGGAGTAGTAACTCCGACTCAGACCTTTTCTCCCGCCTGGCCCAAATTCTTCAAAATGGATCTCAGAAACCCCGGAGCACTACTCAGTGCAAGAGTCCAGGATCTCCTCACAATCCAAAAACACCACCCAAGAGTCCAGTTGTCCCTCGCAGGAGTCCCAGTGCCTCTCCTCGAAGCTCATCCTTGCCTCGCACGTCTAGTTCCTCACCATCTAGGGCTGGACGGCCCCACCATGACCAGAGGAGTTCGTCCCCACATCTGGGGAGAAGCAAGTCACCTCCCAGCCACTCAGGATCTTCCTCCTCCAGGAGGTCCTGCCAACAGGAGCATTGCAAACCCAGCAAGAATGGCCTGAAAGGCTCCGGCAGCCTCCACCACCACTCAGCCAGCACTAAAACCCCCCAAGGGAAGAGTAAGCCAGCCAGTAAACTCAGCAGATAGCGGCCGC

Amino Acid Sequence

MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRN
IIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEV
VLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQ
AFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDRE
PLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPA
EAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGLEV
LFQGPGSAMSGGGEQPDILSVGILVKERWKVLRKIGGGGFGEIYDALDML
TRENVALKVESAQQPKQVLKMEVAVLKKLQGKDHVCRFIGCGRNDRFNYV
VMQLQGRNLADLRRSQSRGTFTISTTLRLGRQILESIESIHSVGFLHRDI
KPSNFAMGRFPSTCRKCYMLDFGLARQFTNSCGDVRPPRAVAGFRGTVRY
ASINAHRNREMGRHDDLWSLFYMLVEFVVGQLPWRKIKDKEQVGSIKERY
DHRLMLKHLPPEFSIFLDHISSLDYFTKPDYQLLTSVFDNSIKTFGVIES
DPFDWEKTGNDGSLTTTTTSTTPQLHTRLTPAAIGIANATPIPGDLLREN
TDEVFPDEQLSDGENGIPVGVSPDKLPGSLGHPRPQEKDVWEEMDANKNK
IKLGICKAATEEENSHGQANGLLNAPSLGSPIRVRSEITQPDRDIPLVRK
LRSIHSFELEKRLTLEPKPDTDKFLETCLEKMQKDTSAGKESILPALLHK
PCVPAVSRTDHIWHYDEEYLPDASKPASANTPEQADGGGSNGFIAVNLSS
CKQEIDSKEWVIVDKEQDLQDFRTNEAVGHKTTGSPSDEEPEVLQVLEAS
PQDEKLQLGPWAENDHLKKETSGVVLALSAEGPPTAASEQYTDRLELQPG
AASQFIAATPTSLMEAQAEGPLTAITIPRPSVASTQSTSGSFHCGQQPEK
KDLQPMEPTVELYSPRENFSGLVVTEGEPPSGGSRTDLGLQIDHIGHDML
PNIRESNKSQDLGPKELPDHNRLVVREFENLPGETEEKSILLESDNEDEK
LSRGQHCIEISSLPGDLVIVEKDHSATTEPLDVTKTQTFSVVPNQDKNNE
IMKLLTVGTSEISSRDIDPHVEGQIGQVAEMQKNKISKDDDIMSEDLPGH
QGDLSTFLHQEGKREKITPRNGELFHCVSENEHGAPTRKDMVRSSFVTRH
SRIPVLAQEIDSTLESSSPVSAKEKLLQKKAYQPDLVKLLVEKRQFKSFL
GDLSSASDKLLEEKLATVPAPFCEEEVLTPFSRLTVDSHLSRSAEDSFLS
PIISQSRKSKIPRPVSWVNTDQVNSSTSSQFFPRPPPGKPPTRPGVEARL
RRYKVLGSSNSDSDLFSRLAQILQNGSQKPRSTTQCKSPGSPHNPKTPPK
SPVVPRRSPSASPRSSSLPRTSSSSPSRAGRPHHDQRSSSPHLGRSKSPP
SHSGSSSSRRSCQQEHCKPSKNGLKGSGSLHHHSASTKTPQGKSKPASKL
SR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU44616
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Precission cleavage. Internal TTBK2 BamHI site KO'd, this fragment may be excised BamHI/NotI for directional downstream cloning work.