cDNA Clone FIP200/ATG13/ULK1/ATG101

• Plasmid: pFBDM FIP200/ATG13/GST-ULK1/ATG101
• Parent Plasmid: pFBDM
• Expressed: ATG13(untagged)
• Genbank: NM_014741.4 GI:326807037
• Tag: untagged
• Species: Human

Sequence of Insert

gaattcgaaaccATGGAAACTGATCTCAATTCCCAGGACAGAAAGGACCT
GGACAAGTTTATTAAATTTTTTGCCCTCAAGACTGTCCAAGTGATTGTCC
AGGCTCGGCTTGGTGAAAAGATTTGCACTCGTTCATCATCTTCTCCAACG
GGTTCAGATTGGTTCAACTTAGCAATCAAAGACATCCCAGAGGTTACACA
TGAAGCAAAGAAGGCACTGGCAGGACAGCTGCCTGCAGTCGGGAGGTCCA
TGTGTGTGGAGATTTCACTTAAGACTTCTGAGGGAGATTCCATGGAGCTG
GAAATATGGTGTCTTGAAATGAATGAAAAGTGTGATAAAGAAATCAAAGT
TTCCTACACGGTGTACAACAGACTGTCATTGCTGCTGAAGTCCCTTCTTG
CTATAACTAGGGTGACACCAGCCTATAGGCTCTCCAGGAAACAAGGGCAT
GAATATGTCATATTATACAGGATATATTTTGGAGAAGTTCAGCTGAGTGG
CTTAGGAGAAGGCTTCCAGACAGTTCGTGTTGGGACAGTGGGCACCCCTG
TGGGCACCATCACTCTTTCTTGTGCTTACAGAATTAACTTGGCATTCATG
TCTACCAGGCAATTTGAGAGGACCCCACCTATCATGGGGATTATTATTGA
TCACTTTGTGGACCGTCCCTATCCCAGCTCCTCTCCCATGCACCCCTGCA
ATTACAGAACTGCTGGTGAGGACACTGGAGTAATATACCCGTCTGTAGAA
GACTCTCAAGAAGTGTGTACCACCTCTTTTTCCACCTCCCCACCATCCCA
GCTGATGGTTCCTGGGAAGGAAGGTGGGGTACCCCTTGCTCCCAACCAGC
CTGTCCATGGTACCCAGGCTGACCAGGAGAGACTGGCAACCTGCACCCCT
TCTGACAGAACCCACTGTGCTGCCACACCCTCCAGTAGTGAGGATACTGA
AACCGTATCAAACAGCAGTGAGGGACGGGCCTCCCCTCACGATGTCTTGG
AGACCATCTTTGTCCGAAAAGTGGGGGCTTTTGTCAACAAACCCATTAAC
CAGGTGACCCTGACGAGTTTGGATATACCCTTTGCCATGTTTGCTCCCAA
GAATTTGGAGCTGGAGGATACCGATCCAATGGTGAATCCTCCAGATTCCC
CAGAGACTGAATCTCCTCTCCAGGGCAGCCTGCACTCAGATGGCTCCAGC
GGGGGCAGCAGTGGCAATACCCATGATGACTTTGTTATGATAGACTTTAA
ACCAGCTTTTTCTAAAGATGACATTCTTCCGATGGACCTGGGGACCTTCT
ATCGGGAGTTTCAGAACCCACCTCAGCTGAGCAGCCTCTCCATAGATATT
GGAGCACAGTCCATGGCTGAAGACTTGGACTCATTACCAGAGAAGCTGGC
TGTGCATGAGAAGAATGTCCGCGAGTTTGATGCCTTTGTGGAAACCCTGC
AGTAAgcggccgc

Amino Acid Sequence

METDLNSQDRKDLDKFIKFFALKTVQVIVQARLGEKICTRSSSSPTGSDW
FNLAIKDIPEVTHEAKKALAGQLPAVGRSMCVEISLKTSEGDSMELEIWC
LEMNEKCDKEIKVSYTVYNRLSLLLKSLLAITRVTPAYRLSRKQGHEYVI
LYRIYFGEVQLSGLGEGFQTVRVGTVGTPVGTITLSCAYRINLAFMSTRQ
FERTPPIMGIIIDHFVDRPYPSSSPMHPCNYRTAGEDTGVIYPSVEDSQE
VCTTSFSTSPPSQLMVPGKEGGVPLAPNQPVHGTQADQERLATCTPSDRT
HCAATPSSSEDTETVSNSSEGRASPHDVLETIFVRKVGAFVNKPINQVTL
TSLDIPFAMFAPKNLELEDTDPMVNPPDSPETESPLQGSLHSDGSSGGSS
GNTHDDFVMIDFKPAFSKDDILPMDLGTFYREFQNPPQLSSLSIDIGAQS
MAEDLDSLPEKLAVHEKNVREFDAFVETLQ*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU45711
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: PmeI/AvrII frag from DU45659 cloned into SpeI/NruI of DU40561.; N.B. Grow at 30 degrees.