cDNA Clone plk3

• Plasmid: pCMVHA-1 PLK3
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-PLK3
• Genbank: NM_004073.2
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGGAGCCTGCCGCCGGTTTCCTGTCTCCGCGCCCCTTCCAGCG TGCGGCCGCCGCGCCCGCTCCCCCGGCCGGGCCCGGGCCGCCTCCGAGTG CCTTGCGCGGACCTGAGCTGGAGATGCTGGCCGGGCTACCGACGTCAGAC CCCGGGCGCCTCATCACGGACCCGCGCAGCGGCCGCACCTACCTCAAAGG CCGCTTGTTGGGCAAGGGGGGCTTCGCCCGCTGCTACGAGGCCACTGACA CAGAGACTGGCAGCGCCTACGCTGTCAAAGTCATCCCGCAGAGCCGCGTC GCCAAGCCGCATCAGCGCGAGAAGATCCTAAATGAGATTGAGCTGCACCG AGACCTGCAGCACCGCCACATCGTGCGTTTTTCGCACCACTTTGAGGACG CTGACAACATCTACATTTTCTTGGAGCTCTGCAGCCGAAAGTCCCTGGCC CACATCTGGAAGGCCCGGCACACCCTGTTGGAGCCAGAAGTGCGCTACTA CCTGCGGCAGATCCTTTCTGGCCTCAAGTACTTGCACCAGCGCGGCATCT TGCACCGGGACCTCAAGTTGGGAAATTTTTTCATCACTGAGAACATGGAA CTGAAGGTGGGGGATTTTGGGCTGGCAGCCCGGTTGGAGCCTCCGGAGCA GAGGAAGAAGACCATCTGTGGCACCCCCAACTATGTGGCTCCAGAAGTGC TGCTGAGACAGGGCCACGGCCCTGAGGCGGATGTATGGTCACTGGGCTGT GTCATGTACACGCTGCTCTGCGGGAGCCCTCCCTTTGAGACGGCTGACCT GAAGGAGACGTACCGCTGCATCAAGCAGGTTCACTACACGCTGCCTGCCA GCCTCTCACTGCCTGCCCGGCAGCTCCTGGCCGCCATCCTTCGGGCCTCA CCCCGAGACCGCCCCTCTATTGACCAGATCCTGCGCCATGACTTCTTTAC CAAGGGCTACACCCCCGATCGACTCCCTATCAGCAGCTGCGTGACAGTCC CAGACCTGACACCCCCCAACCCAGCTAGGAGTCTGTTTGCCAAAGTTACC AAGAGCCTCTTTGGCAGAAAGAAGAAGAGTAAGAATCATGCCCAGGAGAG GGATGAGGTCTCCGGTTTGGTGAGCGGCCTCATGCGCACATCCGTTGGCC ATCAGGATGCCAGGCCAGAGGCTCCAGCAGCTTCTGGCCCAGCCCCTGTC AGCCTGGTAGAGACAGCACCTGAAGACAGCTCACCCCGTGGGACACTGGC AAGCAGTGGAGATGGATTTGAAGAAGGTCTGACTGTGGCCACAGTAGTGG AGTCAGCCCTTTGTGCTCTGAGAAATTGTATAGCCTTCATGCCCCCAGCG GAACAGAACCCGGCCCCCCTGGCCCAGCCAGAGCCTCTGGTGTGGGTCAG CAAGTGGGTTGACTACTCCAATAAGTTCGGCTTTGGGTATCAACTGTCCA GCCGCCGTGTGGCTGTGCTCTTCAACGATGGCACACATATGGCCCTGTCG GCCAACAGAAAGACTGTGCACTACAATCCCACCAGCACAAAGCACTTCTC CTTCTCCGTGGGTGCTGTGCCCCGGGCCCTGCAGCCTCAGCTGGGTATCC TGCGGTACTTCGCCTCCTACATGGAGCAGCACCTCATGAAGGGTGGAGAT CTGCCCAGTGTGGAAGAGGTAGAGGTACCTGCTCCGCCCTTGCTGCTGCA GTGGGTCAAGACGGATCAGGCTCTCCTCATGCTGTTTAGTGATGGCACTG TCCAGGTGAACTTCTACGGGGACCACACCAAGCTGATTCTCAGTGGCTGG GAGCCCCTCCTTGTGACTTTTGTGGCCCGAAATCGTAGTGCTTGTACTTA CCTCGCTTCCCACCTTCGGCAGCTGGGCTGCTCTCCAGACCTGCGGCAGC GACTCCGCTATGCTCTGCGCCTGCTCCGGGACCGCAGCCCAGCCTAGGGA TCC

Amino Acid Sequence

MYPYDVPDYAGSMEPAAGFLSPRPFQRAAAAPAPPAGPGPPPSALRGPEL
EMLAGLPTSDPGRLITDPRSGRTYLKGRLLGKGGFARCYEATDTETGSAY
AVKVIPQSRVAKPHQREKILNEIELHRDLQHRHIVRFSHHFEDADNIYIF
LELCSRKSLAHIWKARHTLLEPEVRYYLRQILSGLKYLHQRGILHRDLKL
GNFFITENMELKVGDFGLAARLEPPEQRKKTICGTPNYVAPEVLLRQGHG
PEADVWSLGCVMYTLLCGSPPFETADLKETYRCIKQVHYTLPASLSLPAR
QLLAAILRASPRDRPSIDQILRHDFFTKGYTPDRLPISSCVTVPDLTPPN
PARSLFAKVTKSLFGRKKKSKNHAQERDEVSGLVSGLMRTSVGHQDARPE
APAASGPAPVSLVETAPEDSSPRGTLASSGDGFEEGLTVATVVESALCAL
RNCIAFMPPAEQNPAPLAQPEPLVWVSKWVDYSNKFGFGYQLSSRRVAVL
FNDGTHMALSANRKTVHYNPTSTKHFSFSVGAVPRALQPQLGILRYFASY
MEQHLMKGGDLPSVEEVEVPAPPLLLQWVKTDQALLMLFSDGTVQVNFYG
DHTKLILSGWEPLLVTFVARNRSACTYLASHLRQLGCSPDLRQRLRYALR
LLRDRSPA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU46139
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: need it in a CMV-based vector for transient transfection. Ta!