cDNA Clone SLX4/SPOP

• Synonyms: BTBD12 (SLX4), BTBD32; TEF2 (SPOP)
• Plasmid: pcDNA5 FRT/TO GFP SLX4 Delta G675-W823 + SPOP C184-A329
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP SLX4 Delta G675-W823 + SPOP C184-A329
• Genbank: SLX4: NM_032444.2 GI:63252862: c3365t P1122L present in other published seqs, and silent changes t732t, c2007a, t4500c. SPOP: NM_003563.3
• Tag: GFP
• Species: Human

Sequence of Insert

atgcatTGCCGGCTGGCAGATGAGTTAGGAGGACTGTGGGAGAATTCCCG
GTTCACAGACTGCTGCTTGTGTGTTGCCGGCCAGGAATTCCAGGCTCACA
AGGCTATCTTAGCAGCTCGTTCTCCGGTTTTTAGTGCCATGTTTGAACAT
GAAATGGAGGAGAGCAAAAAGAATCGAGTTGAAATCAATGATGTGGAGCC
TGAAGTTTTTAAGGAAATGATGTGCTTCATTTACACGGGGAAGGCTCCAA
ACCTCGACAAAATGGCTGATGATTTGCTGGCAGCTGCTGACAAGTATGCC
CTGGAGCGCTTAAAGGTCATGTGTGAGGATGCCCTCTGCAGTAACCTGTC
CGTGGAGAACGCTGCAGAAATTCTCATCCTGGCCGACCTCCACAGTGCAG
ATCAGTTGAAAACTCAGGCAGTGGATTTCATCAACTATCATGCTatgcat

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFIC
TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMKLSV
NEAQLGFYLGSLSHLSACPGIDPRSSEDQPESLKTGQMMDESDEDFKELC
ASFFQRVKKHGIKEVSGERKTQKAASNGTQIRSKLKRTKQTATKTKTLQG
PAEKKPPSGSQAPRTKKQRVTKWQASEPAHSVNGEGGVLASAPDPPVLRE
TAQNTQTGNQQEPSPNLSREKTRENVPNSDSQPPPSCLTTAVPSPSKPRT
AQLVLQRMQQFKRADPERLRHASEECSLEAAREENVPKDPQEEMMAGNVY
GLGPPAPESDAAVALTLQQEFARVGASAHDDSLEEKGLFFCQICQKNLSA
MNVTRREQHVNRCLDEAEKTLRPSVPQIPECPICGKPFLTLKSRTSHLKQ
CAVKMEVGPQLLLQAVRLQTAQPEGSSSPPMFSFSDHSRGLKRRGPTSKK
EPRKRRKVDEAPSEDLLVAMALSRSEMEPGAAVPALRLESAFSERIRPEA
ENKSRKKKPPVSPPLLLVQDSETTGRQIEDRVALLLSEEVELSSTPPLPA
SRILKEGWERAGQCPPPPERKQSFLWEGSALTGAWAMEDFYTARLVPPLV
PQRPAQGLMQEPVPPLVPPEHSELSERRSPALHGTPTAGCGSRGPSPSAS
QREHQALQDLVDLAREGLSASPWPGSGGLAGSEGTAGLDVVPGGLPLTGF
VVPSQDKHPDRGGRTLLSLMHCRLADELGGLWENSRFTDCCLCVAGQEFQ
AHKAILAARSPVFSAMFEHEMEESKKNRVEINDVEPEVFKEMMCFIYTGK
APNLDKMADDLLAAADKYALERLKVMCEDALCSNLSVENAAEILILADLH
SADQLKTQAVDFINYHAMHADEEEEAETLLKSKDHEEDQENVNEAEMEEI
YEFAATQRKLLQEERAAGAGEDADWLEGGSPVSGQLLAGVQVQKQWDKVE
EMEPLEPGRDEAATTWEKMGQCALPPPQGQHSGARGAEAPEQEAPEEALG
HSSCSSPSRDCQAERKEGSLPHSDDAGDYEQLFSSTQGEISEPSQITSEP
EEQSGAVRERGLEVSHRLAPWQASPPHPCRFLLGPPQGGSPRGSHHTSGS
SLSTPRSRGGTSQVGSPTLLSPAVPSKQKRDRSILTLSKEPGHQKGKERR
SVLECRNKGVLMFPEKSLSIDLTQSNPDHSSSRSQKSSSKLNEEDEVILL
LDSDEELELEQTKMKSISSDPLEEKKALEISPRSCELFSIIDVDADQEPS
QSPPRSEAVLQQEDEGALPENRGSLGRRGAPWLFCDRESSPSEASTTDTS
WLVPATPLASRSRDCSSQTQISSLRSGLAVQAVTQHTPRASVGNREGNEV
AQKFSVIRPQTPPPQTPSSCLTPVSPGTSDGRRQGHRSPSRPHPGGHPHS
SPLAPHPISGDRAHFSRRFLKHSPPGPSFLNQTPAGEVVEVGDSDDEQEV
ASHQANRSPPLDSDPPIPIDDCCWHMEPLSPIPIDHWNLERTGPLSTSSP
SRRMNEAADSRDCRSPGLLDTTPIRGSCTTQRKLQEKSSGAGSLGNSRPS
FLNSALWDVWDGEEQRPPETPPPAQMPSAGGAQKPEGLETPKGANRKKNL
PPKVPITPMPQYSIMETPVLKKELDRFGVRPLPKRQMVLKLKEIFQYTHQ
TLDSDSEDESQSSQPLLQAPHCQTLASQTYKPSRAGVHAQQEATTGPGAH
RPKGPAKTKGPRHQRKHHESITPPSRSPTKEAPPGLNDDAQIPASQESVA
TSVDGSDSSLSSQSSSSCEFGAAFESAGEEEGEGEVSASQAAVQAADTDE
ALRCYIRSKPALYQKVLLYQPFELRELQAELRQNGLRVSSRRLLDFLDTH
CITFTTAATRREKLQGRRRQPRGKKKVERN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU45888
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.