cDNA Clone SLX4/FKBP1A

• Synonyms: BTBD12
• Plasmid: pcDNA5 FRT/TO GFP SLX4 Delta G675-W823 + FKBP1A G2-E108 (end) F37V
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP SLX4 Delta G675-W823 + FKBP1A G2-E108 (end) F37V
• Genbank: NM_032444.2 GI:63252862: c3365t P1122L present in other published seqs, and silent changes t732t, c2007a, t4500c.
• Tag: GFP
• Species: Human

Sequence of Insert

atgcatGGAGTGCAGGTGGAGACTATCTCCCCAGGAGACGGGCGCACCTT
CCCCAAGCGCGGCCAGACCTGCGTGGTGCACTACACCGGGATGCTTGAAG
ATGGAAAGAAAGTTGATTCCTCCCGGGACAGAAACAAGCCCTTTAAGTTT
ATGCTAGGCAAGCAGGAGGTGATCCGAGGCTGGGAAGAAGGGGTTGCCCA
GATGAGTGTGGGTCAGAGAGCCAAACTGACTATATCTCCAGATTATGCCT
ATGGTGCCACTGGGCACCCAGGCATCATCCCACCACATGCCACTCTCGTC
TTCGATGTGGAGCTTCTAAAACTGGAAatgcat

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFIC
TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMKLSV
NEAQLGFYLGSLSHLSACPGIDPRSSEDQPESLKTGQMMDESDEDFKELC
ASFFQRVKKHGIKEVSGERKTQKAASNGTQIRSKLKRTKQTATKTKTLQG
PAEKKPPSGSQAPRTKKQRVTKWQASEPAHSVNGEGGVLASAPDPPVLRE
TAQNTQTGNQQEPSPNLSREKTRENVPNSDSQPPPSCLTTAVPSPSKPRT
AQLVLQRMQQFKRADPERLRHASEECSLEAAREENVPKDPQEEMMAGNVY
GLGPPAPESDAAVALTLQQEFARVGASAHDDSLEEKGLFFCQICQKNLSA
MNVTRREQHVNRCLDEAEKTLRPSVPQIPECPICGKPFLTLKSRTSHLKQ
CAVKMEVGPQLLLQAVRLQTAQPEGSSSPPMFSFSDHSRGLKRRGPTSKK
EPRKRRKVDEAPSEDLLVAMALSRSEMEPGAAVPALRLESAFSERIRPEA
ENKSRKKKPPVSPPLLLVQDSETTGRQIEDRVALLLSEEVELSSTPPLPA
SRILKEGWERAGQCPPPPERKQSFLWEGSALTGAWAMEDFYTARLVPPLV
PQRPAQGLMQEPVPPLVPPEHSELSERRSPALHGTPTAGCGSRGPSPSAS
QREHQALQDLVDLAREGLSASPWPGSGGLAGSEGTAGLDVVPGGLPLTGF
VVPSQDKHPDRGGRTLLSLMHGVQVETISPGDGRTFPKRGQTCVVHYTGM
LEDGKKVDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPD
YAYGATGHPGIIPPHATLVFDVELLKLEMHADEEEEAETLLKSKDHEEDQ
ENVNEAEMEEIYEFAATQRKLLQEERAAGAGEDADWLEGGSPVSGQLLAG
VQVQKQWDKVEEMEPLEPGRDEAATTWEKMGQCALPPPQGQHSGARGAEA
PEQEAPEEALGHSSCSSPSRDCQAERKEGSLPHSDDAGDYEQLFSSTQGE
ISEPSQITSEPEEQSGAVRERGLEVSHRLAPWQASPPHPCRFLLGPPQGG
SPRGSHHTSGSSLSTPRSRGGTSQVGSPTLLSPAVPSKQKRDRSILTLSK
EPGHQKGKERRSVLECRNKGVLMFPEKSLSIDLTQSNPDHSSSRSQKSSS
KLNEEDEVILLLDSDEELELEQTKMKSISSDPLEEKKALEISPRSCELFS
IIDVDADQEPSQSPPRSEAVLQQEDEGALPENRGSLGRRGAPWLFCDRES
SPSEASTTDTSWLVPATPLASRSRDCSSQTQISSLRSGLAVQAVTQHTPR
ASVGNREGNEVAQKFSVIRPQTPPPQTPSSCLTPVSPGTSDGRRQGHRSP
SRPHPGGHPHSSPLAPHPISGDRAHFSRRFLKHSPPGPSFLNQTPAGEVV
EVGDSDDEQEVASHQANRSPPLDSDPPIPIDDCCWHMEPLSPIPIDHWNL
ERTGPLSTSSPSRRMNEAADSRDCRSPGLLDTTPIRGSCTTQRKLQEKSS
GAGSLGNSRPSFLNSALWDVWDGEEQRPPETPPPAQMPSAGGAQKPEGLE
TPKGANRKKNLPPKVPITPMPQYSIMETPVLKKELDRFGVRPLPKRQMVL
KLKEIFQYTHQTLDSDSEDESQSSQPLLQAPHCQTLASQTYKPSRAGVHA
QQEATTGPGAHRPKGPAKTKGPRHQRKHHESITPPSRSPTKEAPPGLNDD
AQIPASQESVATSVDGSDSSLSSQSSSSCEFGAAFESAGEEEGEGEVSAS
QAAVQAADTDEALRCYIRSKPALYQKVLLYQPFELRELQAELRQNGLRVS
SRRLLDFLDTHCITFTTAATRREKLQGRRRQPRGKKKVERN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU45890
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.