cDNA Clone SPAK (mouse)

• Synonyms: Stk39, AW227544, AW556857, DCHT, RF005, Rnl5
• Plasmid: pGEX6P-1 mSPAK 424-556
• Parent Plasmid: pGEX6P1
• Expressed: GST-mSPAK 424-556
• Genbank: NM_016866.2
• Tag: GST
• Species: Mouse

Sequence of Insert

GGATCCAACTCAGAGATCTCGGTGAATGCTGGTGGCATCCCTGAGCAAATACAGTCTCTCTCTGTGCACGACTCTCAGGCCCAACCAAACGCTAATGAAGACTACAGAGAAGGTCCTTGTGCAGTCAACCTTGTTTTAAGATTAAGAAACTCCAGAAAGGAACTTAATGACATACGATTTGAGTTTACTCCAGGAAGAGATACAGCAGACGGTGTGTCTCAGGAGCTCTTCTCTGCTGGCTTGGTTGACGGCCATGATGTAGTTATAGTGGCTGCTAATTTACAGAAGATTGTAGATGACCCCAAAGCTTTAAAAACGTTGACATTTAAGTTGGCTTCTGGCTGCGATGGGTCGGAGATTCCTGATGAAGTGAAGCTGATCGGGTTCGCCCAGTTGAGTGTGAGCTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSNSEISVNAGGIPEQIQSLS
VHDSQAQPNANEDYREGPCAVNLVLRLRNSRKELNDIRFEFTPGRDTADG
VSQELFSAGLVDGHDVVIVAANLQKIVDDPKALKTLTFKLASGCDGSEIP
DEVKLIGFAQLSVS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU44891
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 424-556. ; ; All SPAK plasmids MUST be grown at 30 degrees C or less to prevent recombination.