cDNA Clone SLX4/FKBP1A

• Synonyms: BTBD12
• Plasmid: pcDNA5 FRT/TO SLX4 Delta G675-W823 + FKBP1A G2-E108 (end) F37M-HA
• Parent Plasmid: pcDNA5 FRT/TO
• Expressed: SLX4 Delta G675-W823 + FKBP1A G2-E108 (end) F37M-HA
• Genbank: SLX4: NM_032444.2 GI:63252862: c3365t P1122L present in other published seqs, and silent changes t732t, c2007a, t4500c. FKBP1A : NM_000801.4 GI:315139017
• Tag: HA
• Species: Human

Sequence of Insert

ATGCATGGAGTGCAGGTGGAGACTATCTCCCCAGGAGACGGGCGCACCTT CCCCAAGCGCGGCCAGACCTGCGTGGTGCACTACACCGGGATGCTTGAAG ATGGAAAGAAAATGGATTCCTCCCGGGACAGAAACAAGCCCTTTAAGTTT ATGCTAGGCAAGCAGGAGGTGATCCGAGGCTGGGAAGAAGGGGTTGCCCA GATGAGTGTGGGTCAGAGAGCCAAACTGACTATATCTCCAGATTATGCCT ATGGTGCCACTGGGCACCCAGGCATCATCCCACCACATGCCACTCTCGTC TTCGATGTGGAGCTTCTAAAACTGGAAATGCAT

Amino Acid Sequence

MKLSVNEAQLGFYLGSLSHLSACPGIDPRSSEDQPESLKTGQMMDESDED
FKELCASFFQRVKKHGIKEVSGERKTQKAASNGTQIRSKLKRTKQTATKT
KTLQGPAEKKPPSGSQAPRTKKQRVTKWQASEPAHSVNGEGGVLASAPDP
PVLRETAQNTQTGNQQEPSPNLSREKTRENVPNSDSQPPPSCLTTAVPSP
SKPRTAQLVLQRMQQFKRADPERLRHASEECSLEAAREENVPKDPQEEMM
AGNVYGLGPPAPESDAAVALTLQQEFARVGASAHDDSLEEKGLFFCQICQ
KNLSAMNVTRREQHVNRCLDEAEKTLRPSVPQIPECPICGKPFLTLKSRT
SHLKQCAVKMEVGPQLLLQAVRLQTAQPEGSSSPPMFSFSDHSRGLKRRG
PTSKKEPRKRRKVDEAPSEDLLVAMALSRSEMEPGAAVPALRLESAFSER
IRPEAENKSRKKKPPVSPPLLLVQDSETTGRQIEDRVALLLSEEVELSST
PPLPASRILKEGWERAGQCPPPPERKQSFLWEGSALTGAWAMEDFYTARL
VPPLVPQRPAQGLMQEPVPPLVPPEHSELSERRSPALHGTPTAGCGSRGP
SPSASQREHQALQDLVDLAREGLSASPWPGSGGLAGSEGTAGLDVVPGGL
PLTGFVVPSQDKHPDRGGRTLLSLMHGVQVETISPGDGRTFPKRGQTCVV
HYTGMLEDGKKMDSSRDRNKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKL
TISPDYAYGATGHPGIIPPHATLVFDVELLKLEMHADEEEEAETLLKSKD
HEEDQENVNEAEMEEIYEFAATQRKLLQEERAAGAGEDADWLEGGSPVSG
QLLAGVQVQKQWDKVEEMEPLEPGRDEAATTWEKMGQCALPPPQGQHSGA
RGAEAPEQEAPEEALGHSSCSSPSRDCQAERKEGSLPHSDDAGDYEQLFS
STQGEISEPSQITSEPEEQSGAVRERGLEVSHRLAPWQASPPHPCRFLLG
PPQGGSPRGSHHTSGSSLSTPRSRGGTSQVGSPTLLSPAVPSKQKRDRSI
LTLSKEPGHQKGKERRSVLECRNKGVLMFPEKSLSIDLTQSNPDHSSSRS
QKSSSKLNEEDEVILLLDSDEELELEQTKMKSISSDPLEEKKALEISPRS
CELFSIIDVDADQEPSQSPPRSEAVLQQEDEGALPENRGSLGRRGAPWLF
CDRESSPSEASTTDTSWLVPATPLASRSRDCSSQTQISSLRSGLAVQAVT
QHTPRASVGNREGNEVAQKFSVIRPQTPPPQTPSSCLTPVSPGTSDGRRQ
GHRSPSRPHPGGHPHSSPLAPHPISGDRAHFSRRFLKHSPPGPSFLNQTP
AGEVVEVGDSDDEQEVASHQANRSPPLDSDPPIPIDDCCWHMEPLSPIPI
DHWNLERTGPLSTSSPSRRMNEAADSRDCRSPGLLDTTPIRGSCTTQRKL
QEKSSGAGSLGNSRPSFLNSALWDVWDGEEQRPPETPPPAQMPSAGGAQK
PEGLETPKGANRKKNLPPKVPITPMPQYSIMETPVLKKELDRFGVRPLPK
RQMVLKLKEIFQYTHQTLDSDSEDESQSSQPLLQAPHCQTLASQTYKPSR
AGVHAQQEATTGPGAHRPKGPAKTKGPRHQRKHHESITPPSRSPTKEAPP
GLNDDAQIPASQESVATSVDGSDSSLSSQSSSSCEFGAAFESAGEEEGEG
EVSASQAAVQAADTDEALRCYIRSKPALYQKVLLYQPFELRELQAELRQN
GLRVSSRRLLDFLDTHCITFTTAATRREKLQGRRRQPRGKKKVERNYPYD
VPDYA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU45898
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.