cDNA Clone TTBK1

• Synonyms: BDTK
• Plasmid: pET15b TEV HALO TTBK1 1-478 D176A (KD)
• Parent Plasmid: pET15b 6His TEV Halo 3C
• Expressed: 6xHIS-HALO-TTBK1 1-478 D176A (KD)
• Genbank: NM_032538.1
• Tag: 6His
• Tag: HALO
• Species: Human

Sequence of Insert

GGATCCATGCAGTGCCTAGCGGCAGCTCTTAAGGACGAAACCAACATGAG
TGGGGGAGGGGAGCAGGCCGACATCCTGCCGGCCAACTACGTGGTCAAAG
ATCGCTGGAAGGTGCTGAAAAAGATCGGGGGCGGGGGCTTCGGTGAGATC
TATGAGGCCATGGACCTGCTGACCAGGGAGAACGTGGCTCTCAAGGTGGA
GTCAGCCCAACAGCCTAAGCAGGTCCTCAAGATGGAGGTGGCTGTGCTCA
AAAAGCTTCAAGGCAAGGACCACGTGTGCAGGTTCATTGGCTGTGGCCGT
AACGAGAAGTTTAACTATGTGGTGATGCAGCTCCAGGGCCGGAACCTGGC
TGACCTGCGCCGCAGCCAGCCAAGGGGCACTTTCACGTTGAGTACCACAC
TGCGCCTGGGCAAGCAGATCCTGGAGTCCATTGAAGCCATTCACTCCGTG
GGCTTCCTGCACCGTGACATCAAGCCGTCCAACTTTGCCATGGGCCGGCT
GCCCTCGACCTACAGGAAGTGCTACATGTTGGCCTTTGGGCTAGCCAGGC
AGTACACTAACACCACTGGGGACGTCCGGCCTCCTCGGAATGTGGCCGGG
TTCCGGGGGACTGTCCGCTACGCCTCGGTCAATGCTCACAAGAACCGGGA
GATGGGCCGCCATGATGACCTGTGGTCCCTCTTCTACATGCTGGTGGAGT
TTGCTGTGGGCCAGCTGCCCTGGAGGAAGATCAAAGACAAGGAGCAGGTG
GGGATGATCAAAGAGAAATACGAGCACCGGATGCTACTGAAGCACATGCC
ATCCGAGTTTCATCTCTTCCTGGACCACATTGCCAGCCTCGACTACTTCA
CCAAGCCCGATTACCAGTTGATCATGTCAGTGTTTGAGAACAGCATGAAG
GAGCGGGGCATTGCCGAGAACGAGGCCTTTGACTGGGAGAAGGCAGGCAC
TGATGCCCTCCTGTCCACGAGCACCTCCACCCCACCCCAGCAGAACACGC
GACAGACCGCAGCCATGTTTGGAGTGGTCAATGTGACACCAGTGCCAGGG
GATCTGCTGAGGGAAAATACCGAAGACGTGCTCCAGGGGGAACATCTGTC
CGACCAGGAAAACGCTCCACCAATCCTGCCTGGAAGACCATCTGAGGGCC
TCGGACCTAGTCCACACCTGGTGCCACATCCTGGAGGACCAGAAGCAGAA
GTGTGGGAGGAAACCGACGTGAACCGAAATAAGCTGAGGATCAACATTGG
CAAAAGCCCTTGCGTGGAGGAAGAGCAGTCCAGGGGAATGGGTGTGCCAA
GCTCCCCTGTCAGAGCTCCCCCTGATAGCCCAACCACACCCGTGCGCTCC
CTGCGATACAGGAGAGTCAATTCTCCTGAAAGTGAGCGACTCTCTACAGC
AGACGGAAGGGTGGAACTGCCAGAGCGGCGCAGCCGAATGtagcggccgc

Amino Acid Sequence

MGSSHHHHHHSSGLEVLFQGPGSENLYFQGMAEIGTGFPFDPHYVEVLGE
RMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMG
KSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNP
ERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIE
GTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVA
LVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPG
LNLLQEDNPDLIGSEIARWLSTLEISGLEVLFQGPGSMQCLAAALKDETN
MSGGGEQADILPANYVVKDRWKVLKKIGGGGFGEIYEAMDLLTRENVALK
VESAQQPKQVLKMEVAVLKKLQGKDHVCRFIGCGRNEKFNYVVMQLQGRN
LADLRRSQPRGTFTLSTTLRLGKQILESIEAIHSVGFLHRDIKPSNFAMG
RLPSTYRKCYMLAFGLARQYTNTTGDVRPPRNVAGFRGTVRYASVNAHKN
REMGRHDDLWSLFYMLVEFAVGQLPWRKIKDKEQVGMIKEKYEHRMLLKH
MPSEFHLFLDHIASLDYFTKPDYQLIMSVFENSMKERGIAENEAFDWEKA
GTDALLSTSTSTPPQQNTRQTAAMFGVVNVTPVPGDLLRENTEDVLQGEH
LSDQENAPPILPGRPSEGLGPSPHLVPHPGGPEAEVWEETDVNRNKLRIN
IGKSPCVEEEQSRGMGVPSSPVRAPPDSPTTPVRSLRYRRVNSPESERLS
TADGRVELPERRSRM*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU44931
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 1-478, D176A (kinase dead)