cDNA Clone TTBK1

• Synonyms: BDTK
• Plasmid: pcDNA5frtTO FLAG TTBK1 D176A (KD)
• Parent Plasmid: pcDNA5D FRT/TO FLAG
• Expressed: FLAG-TTBK1 D176A (KD)
• Genbank: NM_032538.1
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGCAGTGCCTAGCGGCAGCTCTTAAGGACGAAACCAACATGAGTGGGGGAGGGGAGCAGGCCGACATCCTGCCGGCCAACTACGTGGTCAAAGATCGCTGGAAGGTGCTGAAAAAGATCGGGGGCGGGGGCTTCGGTGAGATCTATGAGGCCATGGACCTGCTGACCAGGGAGAACGTGGCTCTCAAGGTGGAGTCAGCCCAACAGCCTAAGCAGGTCCTCAAGATGGAGGTGGCTGTGCTCAAAAAGCTTCAAGGCAAGGACCACGTGTGCAGGTTCATTGGCTGTGGCCGTAACGAGAAGTTTAACTATGTGGTGATGCAGCTCCAGGGCCGGAACCTGGCTGACCTGCGCCGCAGCCAGCCAAGGGGCACTTTCACGTTGAGTACCACACTGCGCCTGGGCAAGCAGATCCTGGAGTCCATTGAAGCCATTCACTCCGTGGGCTTCCTGCACCGTGACATCAAGCCGTCCAACTTTGCCATGGGCCGGCTGCCCTCGACCTACAGGAAGTGCTACATGTTGGCCTTTGGGCTAGCCAGGCAGTACACTAACACCACTGGGGACGTCCGGCCTCCTCGGAATGTGGCCGGGTTCCGGGGGACTGTCCGCTACGCCTCGGTCAATGCTCACAAGAACCGGGAGATGGGCCGCCATGATGACCTGTGGTCCCTCTTCTACATGCTGGTGGAGTTTGCTGTGGGCCAGCTGCCCTGGAGGAAGATCAAAGACAAGGAGCAGGTGGGGATGATCAAAGAGAAATACGAGCACCGGATGCTACTGAAGCACATGCCATCCGAGTTTCATCTCTTCCTGGACCACATTGCCAGCCTCGACTACTTCACCAAGCCCGATTACCAGTTGATCATGTCAGTGTTTGAGAACAGCATGAAGGAGCGGGGCATTGCCGAGAACGAGGCCTTTGACTGGGAGAAGGCAGGCACTGATGCCCTCCTGTCCACGAGCACCTCCACCCCACCCCAGCAGAACACGCGACAGACCGCAGCCATGTTTGGAGTGGTCAATGTGACACCAGTGCCAGGGGATCTGCTGAGGGAAAATACCGAAGACGTGCTCCAGGGGGAACATCTGTCCGACCAGGAAAACGCTCCACCAATCCTGCCTGGAAGACCATCTGAGGGCCTCGGACCTAGTCCACACCTGGTGCCACATCCTGGAGGACCAGAAGCAGAAGTGTGGGAGGAAACCGACGTGAACCGAAATAAGCTGAGGATCAACATTGGCAAAAGCCCTTGCGTGGAGGAAGAGCAGTCCAGGGGAATGGGTGTGCCAAGCTCCCCTGTCAGAGCTCCCCCTGATAGCCCAACCACACCCGTGCGCTCCCTGCGATACAGGAGAGTCAATTCTCCTGAAAGTGAGCGACTCTCTACAGCAGACGGAAGGGTGGAACTGCCAGAGCGGCGCAGCCGAATGGATCTCCCTGGCAGTCCATCACGACAGGCCTGTTCTAGTCAGCCTGCTCAGATGCTGTCCGTGGACACTGGTCACGCCGATAGGCAGGCTTCTGGCAGAATGGACGTGAGCGCCTCCGTCGAACAGGAGGCACTGTCAAACGCCTTCAGAAGCGTGCCACTCGCCGAAGAGGAAGACTTTGATAGCAAGGAGTGGGTGATCATTGACAAGGAAACCGAGCTGAAAGATTTCCCACCAGGAGCAGAGCCATCTACAAGTGGAACTACCGATGAGGAACCTGAGGAACTGCGGCCACTCCCAGAGGAAGGAGAGGAACGAAGGAGACTGGGTGCAGAGCCTACAGTGCGCCCACGAGGCAGGAGCATGCAGGCACTCGCCGAGGAAGACCTGCAGCACCTCCCTCCACAGCCACTCCCACCTCAGCTGTCCCAGGGAGATGGACGCTCAGAGACCAGCCAGCCACCCACACCCGGTTCACCTAGCCACTCCCCACTGCATTCAGGACCTCGGCCACGGCGCCGAGAGTCCGACCCAACCGGACCACAGCGACAGGTGTTCTCCGTCGCTCCTCCATTTGAGGTCAATGGACTGCCAAGGGCAGTCCCTCTCAGCCTGCCATACCAGGATTTCAAGAGAGACCTGTCCGATTATAGAGAGCGAGCACGACTGCTCAACCGGGTGAGGAGAGTCGGATTTTCTCATATGCTGCTCACAACTCCTCAGGTGCCACTGGCACCCGTCCAGCCTCAGGCCAATGGGAAAGAGGAGGAAGAAGAGGAAGAGGAAGACGAGGAAGAGGAAGAGGAAGATGAAGAGGAAGAGGAAGAGGAGGAGGAGGAGGAGGAAGAAGAAGAAGAGGAGGAGGAGGAAGAGGAGGAGGCCGCTGCAGCCGTGGCCCTCGGAGAGGTCCTGGGGCCTAGAAGCGGTTCAAGCTCCGAAGGCAGCGAGAGATCTACTGACAGGAGCCAGGAGGGAGCTCCAAGCACCCTGCTCGCAGACGATCAGAAGGAGTCCCGAGGCAGGGCCTCTATGGCTGACGGAGATCTGGAGCCTGAAGAGGGGTCTAAAACTCTCGTGCTGGTCAGTCCAGGCGACATGAAGAAATCACCCGTGACCGCAGAGCTCGCACCCGACCCTGATCTGGGAACTCTCGCTGCACTGACCCCACAGCACGAGAGGCCACAGCCAACAGGTAGTCAGCTGGACGTGAGCGAACCTGGCACTCTCTCTAGTGTCCTGAAGTCCGAGCCAAAACCACCTGGACCTGGTGCTGGACTGGGAGCAGGGACTGTGACCACAGGAGTCGGGGGTGTGGCTGTCACATCAAGCCCTTTCACTAAGGTGGAACGCACCTTTGTCCACATCGCCGAGAAAACCCATCTGAACGTGATGTCCTCTGGCGGACAGGCTCTCAGGAGCGAAGAGTTTTCCGCAGGAGGTGAACTCGGACTGGAGCTCGCTTCCGACGGAGGAGCAGTGGAAGAGGGAGCTAGAGCACCCCTGGAGAACGGGCTGGCCCTCTCTGGACTCAATGGGGCTGAAATTGAGGGCAGTGCCCTGTCAGGAGCTCCACGAGAAACACCTTCCGAGATGGCTACTAACTCTCTCCCAAATGGACCCGCCCTGGCTGATGGACCTGCCCCAGTGAGCCCACTGGAACCATCCCCTGAGAAGGTCGCAACCATCAGCCCTCGGCGCCATGCTATGCCAGGCTCTAGACCCCGGAGTCGCATTCCTGTGCTGCTCAGTGAAGAGGACACAGGGTCAGAACCATCTGGTAGTCTGTCAGCCAAGGAGCGGTGGTCTAAACGAGCAAGGCCTCAGCAGGATCTCGCCCGCCTGGTGATGGAGAAGAGGCAGGGAAGACTGCTCCTGAGGCTGGCTAGCGGGGCAAGTTCAAGCTCCTCTGAAGAGCAGCGAAGGGCTTCAGAGACACTGAGCGGTACTGGCTCCGAAGAGGACACACCTGCATCCGAGCCAGCCGCTGCACTGCCACGAAAATCTGGCCGAGCCGCTGCAACTAGAAGTAGAATCCCCCGGCCCATTGGACTGCGCATGCCTATGCCAGTGGCCGCTCAGCAGCCTGCTAGCAGATCCCACGGAGCAGCACCAGCACTGGACACTGCCATCACCTCTCGACTGCAGCTCCAGACCCCACCAGGCAGTGCCACAGCTGCAGATCTGAGGCCAAAGCAGCCTCCAGGACGAGGACTCGGTCCAGGAAGGGCACAGGCAGGAGCTAGACCCCCTGCACCTCGGTCCCCACGACTGCCAGCATCTACAAGTGCCGCTAGAAACGCATCAGCCAGCCCACGGTCCCAGTCTCTGAGTAGACGGGAGTCACCCAGCCCTTCCCATCAGGCACGACCAGGAGTGCCACCACCTCGAGGTGTCCCACCCGCTCGAGCACAGCCAGACGGAACCCCATCTCCAGGAGGTAGTAAGAAAGGTCCCAGGGGCAAGCTGCAGGCCCAGAGGGCAACTACTAAAGGTCGGGCAGGAGGTGCTGAAGGAAGGGCAGGGGCAAGGTGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSMQCLAAALKDETNMSGGGEQADILPANYVVKDRWKVLKK
IGGGGFGEIYEAMDLLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKDH
VCRFIGCGRNEKFNYVVMQLQGRNLADLRRSQPRGTFTLSTTLRLGKQIL
ESIEAIHSVGFLHRDIKPSNFAMGRLPSTYRKCYMLAFGLARQYTNTTGD
VRPPRNVAGFRGTVRYASVNAHKNREMGRHDDLWSLFYMLVEFAVGQLPW
RKIKDKEQVGMIKEKYEHRMLLKHMPSEFHLFLDHIASLDYFTKPDYQLI
MSVFENSMKERGIAENEAFDWEKAGTDALLSTSTSTPPQQNTRQTAAMFG
VVNVTPVPGDLLRENTEDVLQGEHLSDQENAPPILPGRPSEGLGPSPHLV
PHPGGPEAEVWEETDVNRNKLRINIGKSPCVEEEQSRGMGVPSSPVRAPP
DSPTTPVRSLRYRRVNSPESERLSTADGRVELPERRSRMDLPGSPSRQAC
SSQPAQMLSVDTGHADRQASGRMDVSASVEQEALSNAFRSVPLAEEEDFD
SKEWVIIDKETELKDFPPGAEPSTSGTTDEEPEELRPLPEEGEERRRLGA
EPTVRPRGRSMQALAEEDLQHLPPQPLPPQLSQGDGRSETSQPPTPGSPS
HSPLHSGPRPRRRESDPTGPQRQVFSVAPPFEVNGLPRAVPLSLPYQDFK
RDLSDYRERARLLNRVRRVGFSHMLLTTPQVPLAPVQPQANGKEEEEEEE
EDEEEEEEDEEEEEEEEEEEEEEEEEEEEEEEAAAAVALGEVLGPRSGSS
SEGSERSTDRSQEGAPSTLLADDQKESRGRASMADGDLEPEEGSKTLVLV
SPGDMKKSPVTAELAPDPDLGTLAALTPQHERPQPTGSQLDVSEPGTLSS
VLKSEPKPPGPGAGLGAGTVTTGVGGVAVTSSPFTKVERTFVHIAEKTHL
NVMSSGGQALRSEEFSAGGELGLELASDGGAVEEGARAPLENGLALSGLN
GAEIEGSALSGAPRETPSEMATNSLPNGPALADGPAPVSPLEPSPEKVAT
ISPRRHAMPGSRPRSRIPVLLSEEDTGSEPSGSLSAKERWSKRARPQQDL
ARLVMEKRQGRLLLRLASGASSSSSEEQRRASETLSGTGSEEDTPASEPA
AALPRKSGRAAATRSRIPRPIGLRMPMPVAAQQPASRSHGAAPALDTAIT
SRLQLQTPPGSATAADLRPKQPPGRGLGPGRAQAGARPPAPRSPRLPAST
SAARNASASPRSQSLSRRESPSPSHQARPGVPPPRGVPPARAQPDGTPSP
GGSKKGPRGKLQAQRATTKGRAGGAEGRAGAR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU44933
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: D176A (kinase dead)