cDNA Clone SLX4/BCL6

• Synonyms: BTBD12
• Plasmid: pcDNA5 FRT/TO SLX4 Delta G675-W823 + BCL6 L22-K126-HA
• Parent Plasmid: pcDNA5 FRT/TO
• Expressed: SLX4 Delta G675-W823 + BCL6 L22-K126-HA
• Genbank: SLX4: NM_032444.2 GI:63252862: c3365t P1122L present in other published seqs, and silent changes t732t, c2007a, t4500c. BCL6: NM_001706.4 GI:299115782
• Tag: HA
• Species: Human

Sequence of Insert

ATGCATCTTAATCGTCTCCGGAGTCGAGACATCTTGACTGATGTTGTCATTGTTGTGAGCCGTGAGCAGTTTAGAGCCCATAAAACGGTCCTCATGGCCTGCAGTGGCCTGTTCTATAGCATCTTTACAGACCAGTTGAAATGCAACCTTAGTGTGATCAATCTAGATCCTGAGATCAACCCTGAGGGATTCTGCATCCTCCTGGACTTCATGTACACATCTCGGCTCAATTTGCGGGAGGGCAACATCATGGCTGTGATGGCCACGGCTATGTACCTGCAGATGGAGCATGTTGTGGACACTTGCCGGAAGTTTATTAAGATGCAT

Amino Acid Sequence

MKLSVNEAQLGFYLGSLSHLSACPGIDPRSSEDQPESLKTGQMMDESDED
FKELCASFFQRVKKHGIKEVSGERKTQKAASNGTQIRSKLKRTKQTATKT
KTLQGPAEKKPPSGSQAPRTKKQRVTKWQASEPAHSVNGEGGVLASAPDP
PVLRETAQNTQTGNQQEPSPNLSREKTRENVPNSDSQPPPSCLTTAVPSP
SKPRTAQLVLQRMQQFKRADPERLRHASEECSLEAAREENVPKDPQEEMM
AGNVYGLGPPAPESDAAVALTLQQEFARVGASAHDDSLEEKGLFFCQICQ
KNLSAMNVTRREQHVNRCLDEAEKTLRPSVPQIPECPICGKPFLTLKSRT
SHLKQCAVKMEVGPQLLLQAVRLQTAQPEGSSSPPMFSFSDHSRGLKRRG
PTSKKEPRKRRKVDEAPSEDLLVAMALSRSEMEPGAAVPALRLESAFSER
IRPEAENKSRKKKPPVSPPLLLVQDSETTGRQIEDRVALLLSEEVELSST
PPLPASRILKEGWERAGQCPPPPERKQSFLWEGSALTGAWAMEDFYTARL
VPPLVPQRPAQGLMQEPVPPLVPPEHSELSERRSPALHGTPTAGCGSRGP
SPSASQREHQALQDLVDLAREGLSASPWPGSGGLAGSEGTAGLDVVPGGL
PLTGFVVPSQDKHPDRGGRTLLSLMHLNRLRSRDILTDVVIVVSREQFRA
HKTVLMACSGLFYSIFTDQLKCNLSVINLDPEINPEGFCILLDFMYTSRL
NLREGNIMAVMATAMYLQMEHVVDTCRKFIKMHADEEEEAETLLKSKDHE
EDQENVNEAEMEEIYEFAATQRKLLQEERAAGAGEDADWLEGGSPVSGQL
LAGVQVQKQWDKVEEMEPLEPGRDEAATTWEKMGQCALPPPQGQHSGARG
AEAPEQEAPEEALGHSSCSSPSRDCQAERKEGSLPHSDDAGDYEQLFSST
QGEISEPSQITSEPEEQSGAVRERGLEVSHRLAPWQASPPHPCRFLLGPP
QGGSPRGSHHTSGSSLSTPRSRGGTSQVGSPTLLSPAVPSKQKRDRSILT
LSKEPGHQKGKERRSVLECRNKGVLMFPEKSLSIDLTQSNPDHSSSRSQK
SSSKLNEEDEVILLLDSDEELELEQTKMKSISSDPLEEKKALEISPRSCE
LFSIIDVDADQEPSQSPPRSEAVLQQEDEGALPENRGSLGRRGAPWLFCD
RESSPSEASTTDTSWLVPATPLASRSRDCSSQTQISSLRSGLAVQAVTQH
TPRASVGNREGNEVAQKFSVIRPQTPPPQTPSSCLTPVSPGTSDGRRQGH
RSPSRPHPGGHPHSSPLAPHPISGDRAHFSRRFLKHSPPGPSFLNQTPAG
EVVEVGDSDDEQEVASHQANRSPPLDSDPPIPIDDCCWHMEPLSPIPIDH
WNLERTGPLSTSSPSRRMNEAADSRDCRSPGLLDTTPIRGSCTTQRKLQE
KSSGAGSLGNSRPSFLNSALWDVWDGEEQRPPETPPPAQMPSAGGAQKPE
GLETPKGANRKKNLPPKVPITPMPQYSIMETPVLKKELDRFGVRPLPKRQ
MVLKLKEIFQYTHQTLDSDSEDESQSSQPLLQAPHCQTLASQTYKPSRAG
VHAQQEATTGPGAHRPKGPAKTKGPRHQRKHHESITPPSRSPTKEAPPGL
NDDAQIPASQESVATSVDGSDSSLSSQSSSSCEFGAAFESAGEEEGEGEV
SASQAAVQAADTDEALRCYIRSKPALYQKVLLYQPFELRELQAELRQNGL
RVSSRRLLDFLDTHCITFTTAATRREKLQGRRRQPRGKKKVERNYPYDVP
DYA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU45934
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.