cDNA Clone SLX4/SKP1

• Synonyms: BTBD12
• Plasmid: pcDNA5D FRT/TO GFP SLX4 Delta G675-W823 + SKP1
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP SLX4 Delta G675-W823 + SKP1
• Genbank: NM_032444.2 GI:63252862: c3365t P1122L present in other published seqs, and silent changes t732t, c2007a, t4500c.
• Tag: GFP
• Species: Human

Sequence of Insert

ATGCATATGCCTTCAATTAAGTTGCAGAGTTCTGATGGAGAGATATTTGAAGTTGATGTGGAAATTGCCAAACAATCTGTGACTATTAAGACCATGTTGGAAGATTTGGGAATGGATGATGAAGGAGATGATGACCCAGTTCCTCTACCAAATGTGAATGCAGCAATATTAAAAAAGGTCATTCAGTGGTGCACCCACCACAAGGATGACCCTCCTCCTCCTGAAGATGATGAGAACAAAGAAAAGCGAACAGATGATATCCCTGTTTGGGACCAAGAATTCCTGAAAGTTGACCAAGGAACACTTTTTGAACTCATTCTGGCTGCAAACTACTTAGACATCAAAGGTTTGCTTGATGTTACATGCAAGACTGTTGCCAATATGATCAAGGGGAAAACTCCTGAGGAGATTCGCAAGACCTTCAATATCAAAAATGACTTTACTGAAGAGGAGGAAGCCCAGGTACGCAAAGAGAACCAGTGGTGTGAAGAGAAGATGCAT

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFIC
TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMKLSV
NEAQLGFYLGSLSHLSACPGIDPRSSEDQPESLKTGQMMDESDEDFKELC
ASFFQRVKKHGIKEVSGERKTQKAASNGTQIRSKLKRTKQTATKTKTLQG
PAEKKPPSGSQAPRTKKQRVTKWQASEPAHSVNGEGGVLASAPDPPVLRE
TAQNTQTGNQQEPSPNLSREKTRENVPNSDSQPPPSCLTTAVPSPSKPRT
AQLVLQRMQQFKRADPERLRHASEECSLEAAREENVPKDPQEEMMAGNVY
GLGPPAPESDAAVALTLQQEFARVGASAHDDSLEEKGLFFCQICQKNLSA
MNVTRREQHVNRCLDEAEKTLRPSVPQIPECPICGKPFLTLKSRTSHLKQ
CAVKMEVGPQLLLQAVRLQTAQPEGSSSPPMFSFSDHSRGLKRRGPTSKK
EPRKRRKVDEAPSEDLLVAMALSRSEMEPGAAVPALRLESAFSERIRPEA
ENKSRKKKPPVSPPLLLVQDSETTGRQIEDRVALLLSEEVELSSTPPLPA
SRILKEGWERAGQCPPPPERKQSFLWEGSALTGAWAMEDFYTARLVPPLV
PQRPAQGLMQEPVPPLVPPEHSELSERRSPALHGTPTAGCGSRGPSPSAS
QREHQALQDLVDLAREGLSASPWPGSGGLAGSEGTAGLDVVPGGLPLTGF
VVPSQDKHPDRGGRTLLSLMHMPSIKLQSSDGEIFEVDVEIAKQSVTIKT
MLEDLGMDDEGDDDPVPLPNVNAAILKKVIQWCTHHKDDPPPPEDDENKE
KRTDDIPVWDQEFLKVDQGTLFELILAANYLDIKGLLDVTCKTVANMIKG
KTPEEIRKTFNIKNDFTEEEEAQVRKENQWCEEKMHADEEEEAETLLKSK
DHEEDQENVNEAEMEEIYEFAATQRKLLQEERAAGAGEDADWLEGGSPVS
GQLLAGVQVQKQWDKVEEMEPLEPGRDEAATTWEKMGQCALPPPQGQHSG
ARGAEAPEQEAPEEALGHSSCSSPSRDCQAERKEGSLPHSDDAGDYEQLF
SSTQGEISEPSQITSEPEEQSGAVRERGLEVSHRLAPWQASPPHPCRFLL
GPPQGGSPRGSHHTSGSSLSTPRSRGGTSQVGSPTLLSPAVPSKQKRDRS
ILTLSKEPGHQKGKERRSVLECRNKGVLMFPEKSLSIDLTQSNPDHSSSR
SQKSSSKLNEEDEVILLLDSDEELELEQTKMKSISSDPLEEKKALEISPR
SCELFSIIDVDADQEPSQSPPRSEAVLQQEDEGALPENRGSLGRRGAPWL
FCDRESSPSEASTTDTSWLVPATPLASRSRDCSSQTQISSLRSGLAVQAV
TQHTPRASVGNREGNEVAQKFSVIRPQTPPPQTPSSCLTPVSPGTSDGRR
QGHRSPSRPHPGGHPHSSPLAPHPISGDRAHFSRRFLKHSPPGPSFLNQT
PAGEVVEVGDSDDEQEVASHQANRSPPLDSDPPIPIDDCCWHMEPLSPIP
IDHWNLERTGPLSTSSPSRRMNEAADSRDCRSPGLLDTTPIRGSCTTQRK
LQEKSSGAGSLGNSRPSFLNSALWDVWDGEEQRPPETPPPAQMPSAGGAQ
KPEGLETPKGANRKKNLPPKVPITPMPQYSIMETPVLKKELDRFGVRPLP
KRQMVLKLKEIFQYTHQTLDSDSEDESQSSQPLLQAPHCQTLASQTYKPS
RAGVHAQQEATTGPGAHRPKGPAKTKGPRHQRKHHESITPPSRSPTKEAP
PGLNDDAQIPASQESVATSVDGSDSSLSSQSSSSCEFGAAFESAGEEEGE
GEVSASQAAVQAADTDEALRCYIRSKPALYQKVLLYQPFELRELQAELRQ
NGLRVSSRRLLDFLDTHCITFTTAATRREKLQGRRRQPRGKKKVERN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU45942
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.; Parental Plasmid pcDNA5 FRT TO GFP SLX4 Delta G675-W823 + NsiI