cDNA Clone SLX4/SKP1

• Synonyms: BTBD12
• Plasmid: pcDNA5 FRT/TO SLX4 Delta G675-W823 + SKP1-HA
• Parent Plasmid: pcDNA5 FRT/TO
• Expressed: SLX4 Delta G675-W823 + SKP1-HA
• Genbank: NM_032444.2 GI:63252862: c3365t P1122L present in other published seqs, and silent changes t732t, c2007a, t4500c. SKP1: NM_170679.2 GI:160420326
• Tag: HA
• Species: Human

Sequence of Insert

ATGCATATGCCTTCAATTAAGTTGCAGAGTTCTGATGGAGAGATATTTGAAGTTGATGTGGAAATTGCCAAACAATCTGTGACTATTAAGACCATGTTGGAAGATTTGGGAATGGATGATGAAGGAGATGATGACCCAGTTCCTCTACCAAATGTGAATGCAGCAATATTAAAAAAGGTCATTCAGTGGTGCACCCACCACAAGGATGACCCTCCTCCTCCTGAAGATGATGAGAACAAAGAAAAGCGAACAGATGATATCCCTGTTTGGGACCAAGAATTCCTGAAAGTTGACCAAGGAACACTTTTTGAACTCATTCTGGCTGCAAACTACTTAGACATCAAAGGTTTGCTTGATGTTACATGCAAGACTGTTGCCAATATGATCAAGGGGAAAACTCCTGAGGAGATTCGCAAGACCTTCAATATCAAAAATGACTTTACTGAAGAGGAGGAAGCCCAGGTACGCAAAGAGAACCAGTGGTGTGAAGAGAAGATGCAT

Amino Acid Sequence

MKLSVNEAQLGFYLGSLSHLSACPGIDPRSSEDQPESLKTGQMMDESDED
FKELCASFFQRVKKHGIKEVSGERKTQKAASNGTQIRSKLKRTKQTATKT
KTLQGPAEKKPPSGSQAPRTKKQRVTKWQASEPAHSVNGEGGVLASAPDP
PVLRETAQNTQTGNQQEPSPNLSREKTRENVPNSDSQPPPSCLTTAVPSP
SKPRTAQLVLQRMQQFKRADPERLRHASEECSLEAAREENVPKDPQEEMM
AGNVYGLGPPAPESDAAVALTLQQEFARVGASAHDDSLEEKGLFFCQICQ
KNLSAMNVTRREQHVNRCLDEAEKTLRPSVPQIPECPICGKPFLTLKSRT
SHLKQCAVKMEVGPQLLLQAVRLQTAQPEGSSSPPMFSFSDHSRGLKRRG
PTSKKEPRKRRKVDEAPSEDLLVAMALSRSEMEPGAAVPALRLESAFSER
IRPEAENKSRKKKPPVSPPLLLVQDSETTGRQIEDRVALLLSEEVELSST
PPLPASRILKEGWERAGQCPPPPERKQSFLWEGSALTGAWAMEDFYTARL
VPPLVPQRPAQGLMQEPVPPLVPPEHSELSERRSPALHGTPTAGCGSRGP
SPSASQREHQALQDLVDLAREGLSASPWPGSGGLAGSEGTAGLDVVPGGL
PLTGFVVPSQDKHPDRGGRTLLSLMHMPSIKLQSSDGEIFEVDVEIAKQS
VTIKTMLEDLGMDDEGDDDPVPLPNVNAAILKKVIQWCTHHKDDPPPPED
DENKEKRTDDIPVWDQEFLKVDQGTLFELILAANYLDIKGLLDVTCKTVA
NMIKGKTPEEIRKTFNIKNDFTEEEEAQVRKENQWCEEKMHADEEEEAET
LLKSKDHEEDQENVNEAEMEEIYEFAATQRKLLQEERAAGAGEDADWLEG
GSPVSGQLLAGVQVQKQWDKVEEMEPLEPGRDEAATTWEKMGQCALPPPQ
GQHSGARGAEAPEQEAPEEALGHSSCSSPSRDCQAERKEGSLPHSDDAGD
YEQLFSSTQGEISEPSQITSEPEEQSGAVRERGLEVSHRLAPWQASPPHP
CRFLLGPPQGGSPRGSHHTSGSSLSTPRSRGGTSQVGSPTLLSPAVPSKQ
KRDRSILTLSKEPGHQKGKERRSVLECRNKGVLMFPEKSLSIDLTQSNPD
HSSSRSQKSSSKLNEEDEVILLLDSDEELELEQTKMKSISSDPLEEKKAL
EISPRSCELFSIIDVDADQEPSQSPPRSEAVLQQEDEGALPENRGSLGRR
GAPWLFCDRESSPSEASTTDTSWLVPATPLASRSRDCSSQTQISSLRSGL
AVQAVTQHTPRASVGNREGNEVAQKFSVIRPQTPPPQTPSSCLTPVSPGT
SDGRRQGHRSPSRPHPGGHPHSSPLAPHPISGDRAHFSRRFLKHSPPGPS
FLNQTPAGEVVEVGDSDDEQEVASHQANRSPPLDSDPPIPIDDCCWHMEP
LSPIPIDHWNLERTGPLSTSSPSRRMNEAADSRDCRSPGLLDTTPIRGSC
TTQRKLQEKSSGAGSLGNSRPSFLNSALWDVWDGEEQRPPETPPPAQMPS
AGGAQKPEGLETPKGANRKKNLPPKVPITPMPQYSIMETPVLKKELDRFG
VRPLPKRQMVLKLKEIFQYTHQTLDSDSEDESQSSQPLLQAPHCQTLASQ
TYKPSRAGVHAQQEATTGPGAHRPKGPAKTKGPRHQRKHHESITPPSRSP
TKEAPPGLNDDAQIPASQESVATSVDGSDSSLSSQSSSSCEFGAAFESAG
EEEGEGEVSASQAAVQAADTDEALRCYIRSKPALYQKVLLYQPFELRELQ
AELRQNGLRVSSRRLLDFLDTHCITFTTAATRREKLQGRRRQPRGKKKVE
RNYPYDVPDYA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU45943
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.