cDNA Clone SLX4/PLZF

• Synonyms: BTBD12
• Plasmid: pcDNA5 FRT/TO GFP SLX4 Delta G675-W823 + PLZF L24-E123
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP SLX4 Delta G675-W823 + PLZF L24-E123
• Genbank: NM_032444.2 GI:63252862: c3365t P1122L present in other published seqs, and silent changes t732t, c2007a, t4500c. `plxf; NM_006006.4 GI:66932930 with silent change t258c
• Tag: GFP
• Species: Human

Sequence of Insert

ATGCATGCCAACCAGATGCGGCTGGCCGGGACTTTGTGCGATGTGGTCAT CATGGTGGACAGCCAGGAGTTCCACGCCCACCGGACGGTGCTGGCCTGCA CCAGCAAGATGTTTGAGATCCTCTTCCACCGCAATAGTCAACACTATACT TTGGACTTCCTCTCGCCAAAGACCTTCCAGCAGATTCTGGAGTACGCATA TACAGCCACGCTGCAAGCCAAGGCGGAGGACCTGGATGACCTGCTGTATG CGGCCGAGATCCTGGAGATCGAGTACCTGGAGGAACAGTGCCTGAAGATG CTGGAGATGCAT

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMKLSVN
EAQLGFYLGSLSHLSACPGIDPRSSEDQPESLKTGQMMDESDEDFKELCA
SFFQRVKKHGIKEVSGERKTQKAASNGTQIRSKLKRTKQTATKTKTLQGP
AEKKPPSGSQAPRTKKQRVTKWQASEPAHSVNGEGGVLASAPDPPVLRET
AQNTQTGNQQEPSPNLSREKTRENVPNSDSQPPPSCLTTAVPSPSKPRTA
QLVLQRMQQFKRADPERLRHASEECSLEAAREENVPKDPQEEMMAGNVYG
LGPPAPESDAAVALTLQQEFARVGASAHDDSLEEKGLFFCQICQKNLSAM
NVTRREQHVNRCLDEAEKTLRPSVPQIPECPICGKPFLTLKSRTSHLKQC
AVKMEVGPQLLLQAVRLQTAQPEGSSSPPMFSFSDHSRGLKRRGPTSKKE
PRKRRKVDEAPSEDLLVAMALSRSEMEPGAAVPALRLESAFSERIRPEAE
NKSRKKKPPVSPPLLLVQDSETTGRQIEDRVALLLSEEVELSSTPPLPAS
RILKEGWERAGQCPPPPERKQSFLWEGSALTGAWAMEDFYTARLVPPLVP
QRPAQGLMQEPVPPLVPPEHSELSERRSPALHGTPTAGCGSRGPSPSASQ
REHQALQDLVDLAREGLSASPWPGSGGLAGSEGTAGLDVVPGGLPLTGFV
VPSQDKHPDRGGRTLLSLMHANQMRLAGTLCDVVIMVDSQEFHAHRTVLA
CTSKMFEILFHRNSQHYTLDFLSPKTFQQILEYAYTATLQAKAEDLDDLL
YAAEILEIEYLEEQCLKMLEMHADEEEEAETLLKSKDHEEDQENVNEAEM
EEIYEFAATQRKLLQEERAAGAGEDADWLEGGSPVSGQLLAGVQVQKQWD
KVEEMEPLEPGRDEAATTWEKMGQCALPPPQGQHSGARGAEAPEQEAPEE
ALGHSSCSSPSRDCQAERKEGSLPHSDDAGDYEQLFSSTQGEISEPSQIT
SEPEEQSGAVRERGLEVSHRLAPWQASPPHPCRFLLGPPQGGSPRGSHHT
SGSSLSTPRSRGGTSQVGSPTLLSPAVPSKQKRDRSILTLSKEPGHQKGK
ERRSVLECRNKGVLMFPEKSLSIDLTQSNPDHSSSRSQKSSSKLNEEDEV
ILLLDSDEELELEQTKMKSISSDPLEEKKALEISPRSCELFSIIDVDADQ
EPSQSPPRSEAVLQQEDEGALPENRGSLGRRGAPWLFCDRESSPSEASTT
DTSWLVPATPLASRSRDCSSQTQISSLRSGLAVQAVTQHTPRASVGNREG
NEVAQKFSVIRPQTPPPQTPSSCLTPVSPGTSDGRRQGHRSPSRPHPGGH
PHSSPLAPHPISGDRAHFSRRFLKHSPPGPSFLNQTPAGEVVEVGDSDDE
QEVASHQANRSPPLDSDPPIPIDDCCWHMEPLSPIPIDHWNLERTGPLST
SSPSRRMNEAADSRDCRSPGLLDTTPIRGSCTTQRKLQEKSSGAGSLGNS
RPSFLNSALWDVWDGEEQRPPETPPPAQMPSAGGAQKPEGLETPKGANRK
KNLPPKVPITPMPQYSIMETPVLKKELDRFGVRPLPKRQMVLKLKEIFQY
THQTLDSDSEDESQSSQPLLQAPHCQTLASQTYKPSRAGVHAQQEATTGP
GAHRPKGPAKTKGPRHQRKHHESITPPSRSPTKEAPPGLNDDAQIPASQE
SVATSVDGSDSSLSSQSSSSCEFGAAFESAGEEEGEGEVSASQAAVQAAD
TDEALRCYIRSKPALYQKVLLYQPFELRELQAELRQNGLRVSSRRLLDFL
DTHCITFTTAATRREKLQGRRRQPRGKKKVERN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU45944
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.