cDNA Clone SLX4/PLZF

• Synonyms: BTBD12
• Plasmid: pcDNA5 FRT/TO SLX4 Delta G675-W823 + PLZF L24-E123-HA
• Parent Plasmid: pcDNA5 FRT/TO
• Expressed: SLX4 Delta G675-W823 + PLZF L24-E123-HA
• Genbank: NM_032444.2 GI:63252862: c3365t P1122L present in other published seqs, and silent changes t732t, c2007a, t4500c. PLZF; NM_006006.4 GI:66932930 with silent change t258c
• Tag: HA
• Species: Human

Sequence of Insert

ATGCATGCCAACCAGATGCGGCTGGCCGGGACTTTGTGCGATGTGGTCAT CATGGTGGACAGCCAGGAGTTCCACGCCCACCGGACGGTGCTGGCCTGCA CCAGCAAGATGTTTGAGATCCTCTTCCACCGCAATAGTCAACACTATACT TTGGACTTCCTCTCGCCAAAGACCTTCCAGCAGATTCTGGAGTACGCATA TACAGCCACGCTGCAAGCCAAGGCGGAGGACCTGGATGACCTGCTGTATG CGGCCGAGATCCTGGAGATCGAGTACCTGGAGGAACAGTGCCTGAAGATG CTGGAGATGCAT

Amino Acid Sequence

MKLSVNEAQLGFYLGSLSHLSACPGIDPRSSEDQPESLKTGQMMDESDED
FKELCASFFQRVKKHGIKEVSGERKTQKAASNGTQIRSKLKRTKQTATKT
KTLQGPAEKKPPSGSQAPRTKKQRVTKWQASEPAHSVNGEGGVLASAPDP
PVLRETAQNTQTGNQQEPSPNLSREKTRENVPNSDSQPPPSCLTTAVPSP
SKPRTAQLVLQRMQQFKRADPERLRHASEECSLEAAREENVPKDPQEEMM
AGNVYGLGPPAPESDAAVALTLQQEFARVGASAHDDSLEEKGLFFCQICQ
KNLSAMNVTRREQHVNRCLDEAEKTLRPSVPQIPECPICGKPFLTLKSRT
SHLKQCAVKMEVGPQLLLQAVRLQTAQPEGSSSPPMFSFSDHSRGLKRRG
PTSKKEPRKRRKVDEAPSEDLLVAMALSRSEMEPGAAVPALRLESAFSER
IRPEAENKSRKKKPPVSPPLLLVQDSETTGRQIEDRVALLLSEEVELSST
PPLPASRILKEGWERAGQCPPPPERKQSFLWEGSALTGAWAMEDFYTARL
VPPLVPQRPAQGLMQEPVPPLVPPEHSELSERRSPALHGTPTAGCGSRGP
SPSASQREHQALQDLVDLAREGLSASPWPGSGGLAGSEGTAGLDVVPGGL
PLTGFVVPSQDKHPDRGGRTLLSLMHANQMRLAGTLCDVVIMVDSQEFHA
HRTVLACTSKMFEILFHRNSQHYTLDFLSPKTFQQILEYAYTATLQAKAE
DLDDLLYAAEILEIEYLEEQCLKMLEMHADEEEEAETLLKSKDHEEDQEN
VNEAEMEEIYEFAATQRKLLQEERAAGAGEDADWLEGGSPVSGQLLAGVQ
VQKQWDKVEEMEPLEPGRDEAATTWEKMGQCALPPPQGQHSGARGAEAPE
QEAPEEALGHSSCSSPSRDCQAERKEGSLPHSDDAGDYEQLFSSTQGEIS
EPSQITSEPEEQSGAVRERGLEVSHRLAPWQASPPHPCRFLLGPPQGGSP
RGSHHTSGSSLSTPRSRGGTSQVGSPTLLSPAVPSKQKRDRSILTLSKEP
GHQKGKERRSVLECRNKGVLMFPEKSLSIDLTQSNPDHSSSRSQKSSSKL
NEEDEVILLLDSDEELELEQTKMKSISSDPLEEKKALEISPRSCELFSII
DVDADQEPSQSPPRSEAVLQQEDEGALPENRGSLGRRGAPWLFCDRESSP
SEASTTDTSWLVPATPLASRSRDCSSQTQISSLRSGLAVQAVTQHTPRAS
VGNREGNEVAQKFSVIRPQTPPPQTPSSCLTPVSPGTSDGRRQGHRSPSR
PHPGGHPHSSPLAPHPISGDRAHFSRRFLKHSPPGPSFLNQTPAGEVVEV
GDSDDEQEVASHQANRSPPLDSDPPIPIDDCCWHMEPLSPIPIDHWNLER
TGPLSTSSPSRRMNEAADSRDCRSPGLLDTTPIRGSCTTQRKLQEKSSGA
GSLGNSRPSFLNSALWDVWDGEEQRPPETPPPAQMPSAGGAQKPEGLETP
KGANRKKNLPPKVPITPMPQYSIMETPVLKKELDRFGVRPLPKRQMVLKL
KEIFQYTHQTLDSDSEDESQSSQPLLQAPHCQTLASQTYKPSRAGVHAQQ
EATTGPGAHRPKGPAKTKGPRHQRKHHESITPPSRSPTKEAPPGLNDDAQ
IPASQESVATSVDGSDSSLSSQSSSSCEFGAAFESAGEEEGEGEVSASQA
AVQAADTDEALRCYIRSKPALYQKVLLYQPFELRELQAELRQNGLRVSSR
RLLDFLDTHCITFTTAATRREKLQGRRRQPRGKKKVERNYPYDVPDYA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU45945
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.