cDNA Clone mouse Chek1

• Synonyms: C85740; Chk1; rad27
• Plasmid: pCAGGS puro 3XFLAG mouse Chek1
• Parent Plasmid: pCAGGS puro 3FLAG
• Expressed: 3XFLAG mouse Chek1
• Genbank: NM_007691.5 GI:262399398 with silent changes t552c and a1293g.
• Tag: 3FLAG
• Species: Mouse

Sequence of Insert

GGATCCATGGCAGTGCCTTTTGTGGAAGACTGGGATTTGGTGCAAACTTTGGGAGAAGGTGCCTATGGAGAAGTTCAACTTGCTGTGAATAGAATAACTGAAGAAGCTGTTGCAGTGAAAATTGTAGACATGAAGCGGGCCATAGACTGTCCAGAAAATATTAAGAAAGAGATCTGCATCAATAAAATGTTAAGCCACGAGAATGTAGTGAAATTCTATGGCCACAGGAGGGAAGGCCATATCCAGTATCTGTTTCTGGAGTACTGTAGTGGAGGAGAACTTTTTGATAGAATTGAGCCAGACATAGGGATGCCTGAACAAGATGCTCAGAGGTTCTTCCACCAACTCATGGCAGGGGTGGTTTATCTTCATGGAATTGGAATAACTCACAGGGATATTAAACCAGAAAACCTCCTCTTGGATGAAAGGGATAACCTCAAAATCTCTGACTTTGGCTTGGCAACGGTATTTCGGCATAATAATCGTGAACGCTTACTGAACAAGATGTGTGGGACTTTACCTTATGTTGCTCCGGAGCTTCTAAAGAGAAAAGAATTCCATGCAGAACCAGTTGATGTTTGGTCCTGTGGAATAGTACTTACTGCAATGTTGGCTGGAGAATTGCCGTGGGACCAGCCCAGTGATAGCTGTCAGGAATATTCTGATTGGAAAGAAAAAAAAACCTATCTCAATCCTTGGAAAAAAATTGATTCTGCTCCTCTGGCTTTGCTTCATAAAATTCTAGTTGAGACTCCATCAGCAAGGATCACCATCCCAGACATTAAGAAAGATAGATGGTACAACAAACCACTTAACAGAGGAGCAAAGAGGCCACGCGCCACATCAGGTGGTATGTCAGAGTCTTCTAGTGGATTCTCTAAGCACATTCATTCCAATTTGGACTTTTCTCCAGTAAATAATGGTTCCAGTGAAGAAACCGTGAAGTTCTCTAGTTCCCAGCCAGAGCCGAGAACAGGGCTTTCCTTGTGGGACACTGGTCCCTCGAACGTGGACAAACTGGTTCAGGGCATCAGTTTTTCCCAGCCTACGTGTCCTGAGCATATGCTTGTAAACAGTCAGTTACTCGGTACCCCTGGATCTTCACAGAACCCCTGGCAGCGCTTGGTCAAAAGGATGACACGATTCTTTACTAAATTGGATGCGGACAAATCTTACCAATGCCTGAAAGAGACCTTCGAGAAGTTGGGCTATCAGTGGAAGAAGAGTTGTATGAATCAGGTTACTGTATCAACAACTGATAGAAGAAACAATAAGTTGATTTTCAAAATAAATTTGGTGGAAATGGATGAGAAGATACTGGTTGACTTCCGACTTTCTAAGGGTGATGGATTAGAGTTCAAGAGACACTTCCTGAAGATTAAAGGGAAGCTCAGCGATGTTGTGAGCAGCCAGAAGGTTTGGTTTCCTGTTACATGAGCGGCCGC

Amino Acid Sequence

MDYKDHDGDYKDHDIDYKDDDDKGSMAVPFVEDWDLVQTLGEGAYGEVQL
AVNRITEEAVAVKIVDMKRAIDCPENIKKEICINKMLSHENVVKFYGHRR
EGHIQYLFLEYCSGGELFDRIEPDIGMPEQDAQRFFHQLMAGVVYLHGIG
ITHRDIKPENLLLDERDNLKISDFGLATVFRHNNRERLLNKMCGTLPYVA
PELLKRKEFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKK
TYLNPWKKIDSAPLALLHKILVETPSARITIPDIKKDRWYNKPLNRGAKR
PRATSGGMSESSSGFSKHIHSNLDFSPVNNGSSEETVKFSSSQPEPRTGL
SLWDTGPSNVDKLVQGISFSQPTCPEHMLVNSQLLGTPGSSQNPWQRLVK
RMTRFFTKLDADKSYQCLKETFEKLGYQWKKSCMNQVTVSTTDRRNNKLI
FKINLVEMDEKILVDFRLSKGDGLEFKRHFLKIKGKLSDVVSSQKVWFPV
T*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU49082
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee