cDNA Clone PLK1

• Synonyms: PLK; STPK13
• Plasmid: pcDNA5 FRT/TO HA2 PLK1 K82R
• Parent Plasmid: pcDNA5 FRT/TO HA2
• Expressed: HA-PLK1 K82R
• Genbank: NM_005030.3 GI:34147632
• Tag: HA
• Species: Human

Sequence of Insert

GCGGCCGCTATGCATATGAGTGCTGCAGTGACTGCAGGGAAGCTGGCACG
GGCACCGGCCGACCCTGGGAAAGCCGGGGTCCCCGGAGTTGCAGCTCCCG
GAGCTCCGGCGGCGGCTCCACCGGCGAAAGAGATCCCGGAGGTCCTAGTG
GACCCACGCAGCCGGCGGCGCTATGTGCGGGGCCGCTTTTTGGGCAAGGG
CGGCTTTGCCAAGTGCTTCGAGATCTCGGACGCGGACACCAAGGAGGTGT
TCGCGGGCAgaATTGTGCCTAAGTCTCTGCTGCTCAAGCCGCACCAGAGG
GAGAAGATGTCCATGGAAATATCCATTCACCGCAGCCTCGCCCACCAGCA
CGTCGTAGGATTCCACGGCTTTTTCGAGGACAACGACTTCGTGTTCGTGG
TGTTGGAGCTCTGCCGCCGGAGGTCTCTCCTGGAGCTGCACAAGAGGAGG
AAAGCCCTGACTGAGCCTGAGGCCCGATACTACCTACGGCAAATTGTGCT
TGGCTGCCAGTACCTGCACCGAAACCGAGTTATTCATCGAGACCTCAAGC
TGGGCAACCTTTTCCTGAATGAAGATCTGGAGGTGAAAATAGGGGATTTT
GGACTGGCAACCAAAGTCGAATATGACGGGGAGAGGAAGAAGACCCTGTG
TGGGACTCCTAATTACATAGCTCCCGAGGTGCTGAGCAAGAAAGGGCACA
GTTTCGAGGTGGATGTGTGGTCCATTGGGTGTATCATGTATACCTTGTTA
GTGGGCAAACCACCTTTTGAGACTTCTTGCCTAAAAGAGACCTACCTCCG
GATCAAGAAGAATGAATACAGTATTCCCAAGCACATCAACCCCGTGGCCG
CCTCCCTCATCCAGAAGATGCTTCAGACAGATCCCACTGCCCGCCCAACC
ATTAACGAGCTGCTTAATGACGAGTTCTTTACTTCTGGCTATATCCCTGC
CCGTCTCCCCATCACCTGCCTGACCATTCCACCAAGGTTTTCGATTGCTC
CCAGCAGCCTGGACCCCAGCAACCGGAAGCCCCTCACAGTCCTCAATAAA
GGCTTGGAGAACCCCCTGCCTGAGCGTCCCCGGGAAAAAGAAGAACCAGT
GGTTCGAGAGACAGGTGAGGTGGTCGACTGCCACCTCAGTGACATGCTGC
AGCAGCTGCACAGTGTCAATGCCTCCAAGCCCTCGGAGCGTGGGCTGGTC
AGGCAAGAGGAGGCTGAGGATCCTGCCTGCATCCCCATCTTCTGGGTCAG
CAAGTGGGTGGACTATTCGGACAAGTACGGCCTTGGGTATCAGCTCTGTG
ATAACAGCGTGGGGGTGCTCTTCAATGACTCAACACGCCTCATCCTCTAC
AATGATGGTGACAGCCTGCAGTACATAGAGCGTGACGGCACTGAGTCCTA
CCTCACCGTGAGTTCCCATCCCAACTCCTTGATGAAGAAGATCACCCTCC
TTAAATATTTCCGCAATTACATGAGCGAGCACTTGCTGAAGGCAGGTGCC
AACATCACGCCGCGCGAAGGTGATGAGCTCGCCCGGCTGCCCTACCTACG
GACCTGGTTCCGCACCCGCAGCGCCATCATCCTGCACCTCAGCAACGGCA
GCGTGCAGATCAACTTCTTCCAGGATCACACCAAGCTCATCTTGTGCCCA
CTGATGGCAGCCGTGACCTACATCGACGAGAAGCGGGACTTCCGCACATA
CCGCCTGAGTCTCCTGGAGGAGTACGGCTGCTGCAAGGAGCTGGCCAGCC
GGCTCCGCTACGCCCGCACTATGGTGGACAAGCTGCTGAGCTCACGCTCG
GCCAGCAACCGTCTCAAGGCCTCCTAAgcggccgc

Amino Acid Sequence

MYPYDVPDYAGIPGIPGSTRAAAMHMSAAVTAGKLARAPADPGKAGVPGV
AAPGAPAAAPPAKEIPEVLVDPRSRRRYVRGRFLGKGGFAKCFEISDADT
KEVFAGRIVPKSLLLKPHQREKMSMEISIHRSLAHQHVVGFHGFFEDNDF
VFVVLELCRRRSLLELHKRRKALTEPEARYYLRQIVLGCQYLHRNRVIHR
DLKLGNLFLNEDLEVKIGDFGLATKVEYDGERKKTLCGTPNYIAPEVLSK
KGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHIN
PVAASLIQKMLQTDPTARPTINELLNDEFFTSGYIPARLPITCLTIPPRF
SIAPSSLDPSNRKPLTVLNKGLENPLPERPREKEEPVVRETGEVVDCHLS
DMLQQLHSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDKYGLGY
QLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKK
ITLLKYFRNYMSEHLLKAGANITPREGDELARLPYLRTWFRTRSAIILHL
SNGSVQINFFQDHTKLILCPLMAAVTYIDEKRDFRTYRLSLLEEYGCCKE
LASRLRYARTMVDKLLSSRSASNRLKAS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU49063
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Kinase dead PLK1 K82R.