cDNA Clone
Brca1 (Mouse)

Plasmid
pCAGGS puro 3XFLAG mouse Brca1 (P659H)
Parent Plasmid
pCAGGS puro 3FLAG
Expressed
3XFLAG mouse Brca1
Genbank
NM_009764.3 GI:161016834 with change c1976a (P659H)
Tag
3FLAG
Species
Mouse
Sequence of Insert
CTCGAGCATGGATTTATCTGCCGTCCAAATTCAAGAAGTACAAAATGTCCTTCATGCTATGCAGAAAATCTTAGAGTGTCCGATCTGTTTGGAACTGATCAAAGAACCTGTTTCCACAAAGTGTGACCACATATTTTGCAAATTTTGTATGCTGAAACTTCTTAACCAGAAGAAAGGGCCTTCACAATGTCCTTTGTGTAAGAATGAGATAACCAAAAGGAGCCTACAGGGAAGCACAAGGTTTAGTCAGCTTGCTGAAGAGCTGCTGAGAATAATGGCTGCTTTTGAGCTTGACACGGGAATGCAGCTTACAAATGGTTTTAGTTTTTCAAAAAAGAGAAATAATTCTTGTGAGCGTTTGAATGAGGAGGCGTCGATCATCCAGAGCGTGGGCTACCGGAACCGTGTCAGAAGGCTTCCCCAGGTCGAACCTGGAAATGCCACCTTGAAGGACAGCCTAGGTGTCCAGCTGTCTAACCTTGGAATCGTGAGATCAGTGAAGAAAAACAGGCAGACCCAACCTCGAAAGAAATCTGTCTACATTGAACTAGACTCTGATTCTTCTGAAGAGACAGTAACTAAGCCAGGTGATTGCAGTGTGAGAGACCAGGAATTGTTACAGACCGCCCCTCAAGAAGCTGGAGATGAAGGCAAGCTGCACTCTGCAGAAGAGGCTGCTTGTGAGTTTTCTGAGGGCATAAGAAACATTGAACATCATCAATGCAGTGATGATTTAAACCCTACTGAGAATCATGCAACTGAAAGGCATCCAGAAAAATGTCAGAGTATTTCTATTTCAAATGTGTGTGTGGAGCCATGTGGCACAGATGCTCATGCCAGCTCATTACAGCCTGAGACCAGCAGTTTATTGCTCATTGAAGACAGAATGAATGCAGAAAAGGCTGAATTCTGTAATAAAAGCAAACAGCCTGGCATAGCAGTGAGCCAGCAGAGCAGATGGGCTGCAAGTAAAGGAACATGTAACGACAGGCAGGTTCCCAGCACTGGGGAAAAGGTAGGTCCAAACGCTGACTCCCTTAGTGATAGAGAGAAGTGGACTCACCCGCAAAGTCTGTGCCCTGAGAATTCTGGAGCTACCACCGATGTTCCTTGGATAACACTAAATAGCAGCGTTCAGAAAGTTAATGAGTGGTTTTCCAGAACTGGTGAAATGTTAACTTCTGACAGCGCATCTGCCAGGAGGCACGAGTCAAATGCTGAAGCAGCTGTTGTGTTGGAAGTTTCAAACGAAGTGGATGGGGGTTTTAGTTCTTCAAGGAAAACAGACTTAGTAACCCCCGACCCCCATCATACTTTAATGTGTAAAAGTGGAAGAGACTTCTCCAAACCAGTAGAGGATAATATCAGTGATAAAATATTTGGGAAATCCTATCAGAGAAAGGGAAGCCGCCCTCACCTGAACCATGTGACTGAAATTATAGGCACATTTATTACAGAACCACAGATAACACAAGAGCAGCCCTTCACAAATAAATTAAAACGTAAGAGAAGTACATCCCTTCAACCTGAGGACTTCATCAAGAAAGCAGATTCAGCAGGTGTTCAAAGGACTCCTGACAACATAAATCAGGGAACTGACCTAATGGAGCCAAATGAGCAAGCAGTGAGTACTACCAGTAACTGTCAGGAGAACAAAATAGCAGGTAGTAATCTCCAGAAAGAGAAAAGCGCTCATCCAACTGAATCATTGAGAAAGGAACCTGCTTCCACAGCAGGAGCCAAATCTATAAGCAACAGTGTAAGTGATTTGGAGGTAGAATTAAACGTCCACAGTTCAAAAGCACCTAAGAAAAATAGGCTGAGGAGGAAGTCTTCTATCAGGTGTGCTCTTCCACTTGAACCAATCAGTAGAAATCCAAGCCCACCTACTTGTGCTGAGCTTCAAATCGATAGTTGTGGTAGCAGTGAAGAAACAAAGAAAAACCATTCCAACCAACAGCCAGCCGGGCACCTTAGAGAGCATCAACTCATCGAAGACACTGAACCTGCAGCGGATGCCAAGAAGAACGAGCCAAATGAACACATAAGGAAGAGACGTGCCAGCGATGCTTTCCCAGAAGAGAAATTAATGAACAAAGCTGGTTTATTAACTAGCTGTTCAAGTCCTAGAAAATCTCAAGGGCCTGTCAATCCCAGCCCTCAGAGAACAGGAACAGAGCAACTTGAAACACGCCAAATGTCTGACAGTGCCAAAGAACTCGGGGATCGGGTCCTAGGAGGAGAGCCCAGTGGCAAAACCACTGACCGATCTGAGGAGAGCACCAGCGTATCCTTGGTATCTGACACTGACTACGACACTCAGAACAGTGTCTCAGTCCTGGACGCTCACACTGTCAGATATGCAAGAACAGGATCCGCTCAGTGTATGACTCAGTTTGTAGCAAGCGAAAACCCCAAGGAACTCGTCCATGGCTCTAACAATGCTGGGAGTGGCACAGAGGGTCTCAAGCCCCCCTTGAGACACGCGCTTAACCTCAGTCAGGAGAAAGTAGAAATGGAAGACAGTGAACTTGATACTCAGTATTTGCAGAATACATTTCAAGTTTCAAAGCGTCAGTCATTTGCTTTATTTTCAAAACCTAGAAGTCCCCAAAAGGACTGTGCTCACTCTGTGCCCTCAAAGGAACTGAGTCCAAAGGTGACAGCTAAAGGTAAACAAAAAGAACGTCAGGGACAGGAAGAATTTGAAATCAGTCACGTACAAGCAGTTGCGGCCACAGTGGGCTTACCTGTGCCCTGTCAAGAAGGTAAGCTAGCTGCTGATACAATGTGTGATAGAGGTTGTAGGCTTTGTCCATCATCTCATTACAGAAGCGGGGAGAATGGACTCAGCGCCACAGGTAAATCAGGAATTTCACAAAACTCACATTTTAAACAATCAGTTTCTCCCATCAGGTCATCTATAAAAACTGACAATAGGAAACCTCTGACAGAGGGACGATTTGAGAGACATACATCATCAACTGAGATGGCGGTGGGAAATGAGAACATTCTTCAGAGTACAGTGCACACAGTTAGCCTGAATAACAGAGGAAATGCTTGTCAAGAAGCCGGCTCGGGCAGTATTCATGAAGTATGTTCCACTGGTGACTCCTTCCCAGGACAACTAGGTAGAAACAGAGGGCCTAAGGTGAACACTGTGCCTCCATTAGATAGTATGCAGCCTGGTGTCTGTCAGCAAAGTGTTCCTGTAAGTGATAAGTATCTTGAAATAAAAAAGCAGGAGGGTGAGGCTGTCTGTGCAGACTTCTCTCCATGTCTATTCTCAGACCATCTTGAGCAATCTATGAGTGGTAAGGTTTTTCAGGTTTGCTCTGAGACACCTGATGACCTGCTGGATGATGTTGAAATACAGGGACATACTAGCTTTGGTGAAGGTGACATAATGGAGAGATCTGCTGTCTTTAACGGAAGCATCCTGAGAAGGGAGTCCAGTAGGAGCCCTAGTCCTGTAACCCATGCATCGAAGTCTCAGAGTCTCCACAGAGCGTCTAGGAAATTAGAATCGTCAGAAGAGAGCGACTCCACTGAGGATGAAGATCTTCCCTGCTTCCAACACTTACTGAGCAGAATAAGCAACACACCTGAGCTTACCAGATGCAGCAGTGCTGTGACACAGCGTATGCCAGAGAAAGCGGAGGGGACCCAAGCACCATGGAAGGGTAGCAGCAGTGACTGCAATAATGAGGTGATCATGATAGAGGCATCTCAGGAGCATCAGTTTAGTGAGGATCCAAGATGCTCTGGCAGCATGTTCTCTTCACAGCACAGTGCTGCCCAAGGGTCAACTGCAAATGCAAACTCCCAGGATTCCAATTTTATTCCACCTTCCAAACAGAGGAGTCACCAGTGTGGGAATGAGGAAGCTTTCCTAAGTGACAAGGAATTGATTTCAGATAACGAGGAAATGGCAACTTGCCTAGAAGAGGATAATGACCAAGAAGAGGATAGTATAATCCCAGATTCAGAGGCATCCGGATACGAGAGTGAAACAAACCTTTCTGAAGACTGCTCGCAGAGTGATATTTTAACCACTCAGCAGCGGGCGACCATGAAGTATAACCTGATAAAGCTGCAGCAGGAAATGGCTCACCTGGAAGCTGTGCTGGAGCAGCGTGGGAACCAGCCTTCTGGCCACTCCCCTTCCCTCCTAGCGGACCCTTGTGCCCTGGAAGACCTGCCAGATCTGGAACCAAACATGTCAGGAGCAGCAATTTTAACTTCAAAGAACATTAATGAGAATCCTGTAAGCCAAAATTTGAAGAGCGCTTGTGATGACAAATTCCAACTACAACATCTGGAGGGTCCCACCAGTGGAGATGACGAGTCAGGGATGGGAAGGCCTTCCCCTTTTAAATCTCCGTTGGCAGGCAGTAGGGGCTCTGCACATGGCTGCTCTAGGCATCTTCAAAAGAGAAACTCCCCCTCTCAGGAGGAGCTCCTCCAGCCTGCTGGATCAGAGGCGTCATCTGAGCCACACAATTCAACAGGGCAGTCTTGCCTGCCAAGGCGAGAGCTAGAAGGAACCCCATACCTGGGATCTGGAATCAGCCTTTTCTCTAGTAGAGACCCCGAATCTGAGTCCCCTAAAGAGCCAGCCCACATTGGCACCACACCAGCTTCAACCTCTGCACTGAAAATACCCCAAGGTCAAGTTGCTTTCCGGAGTGCAGCTGCTGCTGGTGCTGATAAAGCAGTGGTAGGAATTGTGAGCAAGATAAAGCCGGAATTGACATCTTCAGAAGAAAGAGCGGATAGAGACATATCCATGGTGGTGTCAGGCTTGACCCCCAAAGAAGTAATGACCGTGCAAAAGTTTGCTGAAAAATACCGCCTCACTTTAACTGACGCAATTACTGAGGAGACTACACATGTAATTATAAAAACAGATGCGGAGTTTGTGTGTGAGCGGACACTGAAATATTTTCTGGGCATTGCAGGAGGAAAGTGGATAGTTAGCTATTCATGGGTGGTCCGGTCTATCCAAGAAAGAAGACTTCTGAATGTGCATGAATTTGAAGTCAAAGGAGATGTTGTGACTGGAAGAAATCACCAAGGTCCAAGGCGATCCAGAGAATCCCGGGAAAAGCTCTTCAAGGGCCTACAGGTCTATTGTTGTGAGCCCTTCACCAACATGCCCAAAGATGAGCTGGAGAGGATGCTGCAGCTGTGTGGGGCTTCCGTGGTGAAGGAGCTTCCATCGCTCACCCATGACACAGGTGCTCATCTAGTTGTGATCGTGCAGCCAAGCGCCTGGACAGAAGACAGCAACTGCCCAGATATTGGGCAGCTGTGCAAGGCACGTCTTGTGATGTGGGACTGGGTGTTGGACAGTCTATCCAGCTACCGGTGTCGGGATCTGGATGCCTACCTGGTACAGAATATCACCTGTGACAGTAGTGAGCCACAAGACTCCAATGATTAAGCGGCCGC

Amino Acid Sequence
MDYKDHDGDYKDHDIDYKDDDDKGSPEFNSSMDLSAVQIQEVQNVLHAMQ
KILECPICLELIKEPVSTKCDHIFCKFCMLKLLNQKKGPSQCPLCKNEIT
KRSLQGSTRFSQLAEELLRIMAAFELDTGMQLTNGFSFSKKRNNSCERLN
EEASIIQSVGYRNRVRRLPQVEPGNATLKDSLGVQLSNLGIVRSVKKNRQ
TQPRKKSVYIELDSDSSEETVTKPGDCSVRDQELLQTAPQEAGDEGKLHS
AEEAACEFSEGIRNIEHHQCSDDLNPTENHATERHPEKCQSISISNVCVE
PCGTDAHASSLQPETSSLLLIEDRMNAEKAEFCNKSKQPGIAVSQQSRWA
ASKGTCNDRQVPSTGEKVGPNADSLSDREKWTHPQSLCPENSGATTDVPW
ITLNSSVQKVNEWFSRTGEMLTSDSASARRHESNAEAAVVLEVSNEVDGG
FSSSRKTDLVTPDPHHTLMCKSGRDFSKPVEDNISDKIFGKSYQRKGSRP
HLNHVTEIIGTFITEPQITQEQPFTNKLKRKRSTSLQPEDFIKKADSAGV
QRTPDNINQGTDLMEPNEQAVSTTSNCQENKIAGSNLQKEKSAHPTESLR
KEPASTAGAKSISNSVSDLEVELNVHSSKAPKKNRLRRKSSIRCALPLEP
ISRNPSPPTCAELQIDSCGSSEETKKNHSNQQPAGHLREHQLIEDTEPAA
DAKKNEPNEHIRKRRASDAFPEEKLMNKAGLLTSCSSPRKSQGPVNPSPQ
RTGTEQLETRQMSDSAKELGDRVLGGEPSGKTTDRSEESTSVSLVSDTDY
DTQNSVSVLDAHTVRYARTGSAQCMTQFVASENPKELVHGSNNAGSGTEG
LKPPLRHALNLSQEKVEMEDSELDTQYLQNTFQVSKRQSFALFSKPRSPQ
KDCAHSVPSKELSPKVTAKGKQKERQGQEEFEISHVQAVAATVGLPVPCQ
EGKLAADTMCDRGCRLCPSSHYRSGENGLSATGKSGISQNSHFKQSVSPI
RSSIKTDNRKPLTEGRFERHTSSTEMAVGNENILQSTVHTVSLNNRGNAC
QEAGSGSIHEVCSTGDSFPGQLGRNRGPKVNTVPPLDSMQPGVCQQSVPV
SDKYLEIKKQEGEAVCADFSPCLFSDHLEQSMSGKVFQVCSETPDDLLDD
VEIQGHTSFGEGDIMERSAVFNGSILRRESSRSPSPVTHASKSQSLHRAS
RKLESSEESDSTEDEDLPCFQHLLSRISNTPELTRCSSAVTQRMPEKAEG
TQAPWKGSSSDCNNEVIMIEASQEHQFSEDPRCSGSMFSSQHSAAQGSTA
NANSQDSNFIPPSKQRSHQCGNEEAFLSDKELISDNEEMATCLEEDNDQE
EDSIIPDSEASGYESETNLSEDCSQSDILTTQQRATMKYNLIKLQQEMAH
LEAVLEQRGNQPSGHSPSLLADPCALEDLPDLEPNMSGAAILTSKNINEN
PVSQNLKSACDDKFQLQHLEGPTSGDDESGMGRPSPFKSPLAGSRGSAHG
CSRHLQKRNSPSQEELLQPAGSEASSEPHNSTGQSCLPRRELEGTPYLGS
GISLFSSRDPESESPKEPAHIGTTPASTSALKIPQGQVAFRSAAAAGADK
AVVGIVSKIKPELTSSEERADRDISMVVSGLTPKEVMTVQKFAEKYRLTL
TDAITEETTHVIIKTDAEFVCERTLKYFLGIAGGKWIVSYSWVVRSIQER
RLLNVHEFEVKGDVVTGRNHQGPRRSRESREKLFKGLQVYCCEPFTNMPK
DELERMLQLCGASVVKELPSLTHDTGAHLVVIVQPSAWTEDSNCPDIGQL
CKARLVMWDWVLDSLSSYRCRDLDAYLVQNITCDSSEPQDSND*
Vector Type
Mammalian
Antibiotic
Amp
DU Number
DU49073
Location
PPU
MTA
Price
£125.00
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